DRP000016 Comprehensive analysis of polysomal mRNAs in HT29 cell at 16hr after treatment with tunicamycin.
Study Type:
Transcriptome Analysis
Submission:
DRA000016 by on 2009-08-17 14:59:00
Abstract:
Expression profile in HT29 cell exposed to ER stress was attempted. We used shotgun sequencing method, in which next gene sequencing technology and polysome analysis were combined.
Description:
We analyzed the mRNA profile in cytoplasm and in polysome during the endoplasmic reticulum (ER) stress using second generation sequencers. We compared three time points (0 hr/untreatment control, 4 hr and 16 hr after treatment with 2 ﾎｼg/mL ER stress inducible agent Tunicamycin (Tm)). This is the first time mRNA in 窶徘olysome窶・is analyzed using the next generation sequencers and compared with that of cytoplasm.
Center Project:
Integrateve Transcriptome Analysis
NCBI Link:
/pubmed:22465528
BioProject:
34559 (primary)
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Experiments
| Show RUNs for each experimentHide RUNs for each experiment | |||
| ⇑Accession | Spots | Bases | |
|---|---|---|---|
| Total: 1 | 4.1 M | 147.3 M | |
 |
DRX000016 | 4.1 M | 147.3 M |
| DRR000036 | 4.1 M | 147.3 M | |