Agricultural Research Service Date Filed: June 19, 2000 Abstract: The invention is directed to nucleic acid and amino acid sequences for transformation constructs containing piggyBac transposable elements. These constructs allow for the precise excision and insertion of heterologous DNA into a host cell. The invention could be used to genetically modify organisms such as; for example, insect pests by rendering these pests sterile or susceptible to certain controls or beneficial insects to make them pesticide resistant. Inventors: Paul D. Shirk Center for Medical and Agricultural Veterinary Entomology Gainesville, FL 32608-1069 (352) 374-5720 E-mail: pshirk@gainesville.usda.ufl.edu Malcolm J. Fraser, Jr. Department of Biological Science University of Notre Dame Notre Dame, IN 46556 Omaththage Perera University of Florida Gainesville, FL 32608 Teresa A. Elick-Beam Department of Biology University of St. Francis Fort Wayne, IN 46808 Industry Keywords: insect control, nucleic acids, genes, DNA/RNA, organisms, genetic engineering, biological control, sterile, environment, insecticide, integrated pest management, transposable elements, piggyBac *CIP Patent Application 08/844,274, filed April 28, 1997.