Non-enzymatic glycation of serum apolipoproteins is a main feature of diabetes mellitus under hyperglycemia. Advanced glycation end products are implicated in the development of aging and metabolic syndrome, including premature atherosclerosis in diabetic subjects. ApoA-I is the principal protein constituent of HDL. In this study, glycated human apoA-I ...

High-density lipoprotein (HDL) is a strong antioxidant, anti-inflammatory, and antisenescence molecule. However, in the current study, HDL from the elderly group (E-HDL) exhibited increased glycation with apolipoprotein (apo) A-I multimerization and decreased phospholipid content. Similarly, glycated apoA-I (gA-I) by fructosylation has ...

The aim of this study was to evaluate the ability of plasma levels of apo A-I and apo B to discriminate between male patients with and without angiographically determined coronary artery disease (CAD) in comparison to the levels of cholesterol, HDL-cholesterol and triglycerides. The plasma apo A-I and B levels were measured by a radial immunodiffusion ...

For several decades, the standard model for high density lipoprotein (HDL) particles reconstituted from apolipoprotein A-I (apoA-I) and phospholipid (apoA-I/HDL) has been a discoidal particle ?100 ? in diameter and the thickness of a phospholipid bilayer. Recently, Wu et al. (Wu, Z., Gogonea, V., Lee, X., Wagner, ...

We performed alignment of apolipoprotein A-I (apoA-I) sequences from 31 species of animals. We found there is specific conservation of salt bridge-forming residues in the first 30 residues of apoA-I and general conservation of a variety of residue types in the central domain, helix 2/3 to helix 7/8. In the lipid-associating domain, helix 7 and helix 10 are ...

Since the mid-1980's, several A.I.D. Missions and host governments in Latin America and the Caribbean have worked together to establish endowments for existing and new institutions. While many of these endowments are being used to strengthen the instituti...

ObjectiveThe purpose of this study was to determine the effects of an atherogenic diet on immune function in LDLr^?/?, ApoA-I^?/? mice.Methods and ResultsWhen LDLr^?/?, ApoA-I^?/? (DKO) and LDLr^?/? (SKO) mice were fed an atherogenic diet DKO had ...

We have identified a novel mutation in apoA-I (serine 36 to alanine; S36A) in a human subject with severe hypoalphalipoproteinemia. The mutation is located in the N-terminal region of the protein, which has been implicated in several functions, including lipid binding and lecithin:cholesterol acyltransferase (LCAT) activity. In the present study, the S36A ...

We have identified a novel mutation in apoA-I (serine 36 to alanine; S36A) in a human subject with severe hypoalphalipoproteinemia. The mutation is located in the N-terminal region of the protein, which has been implicated in several functions, including lipid binding and lecithin:cholesterol acyltransferase (LCAT) activity. In the present study, the S36A ...

The MAPS (Private Sector Development) framework was used to assess A.I.D.'s private sector strategy for the Dominican Republic. Results show the Dominican private sector to be surprisingly strong and diversified. It justified A.I.D.'s strategy, which has ...

In order to study the regulation of the promoter of apolipoprotein (apo) A-I gene, they joined the 5' end of rat apo A-I gene (1.9 Kb) to the coding region of bacterial chloramphenicol acetyltransferase (CAT) gene. The chimeric gene produced high levels of CAT activity in both mouse L cells and Hep G2 cells in transient expression assays. Ethanol ...

apo B is a structural constituent of several classes of lipoprotein particles, including chylomicrons, VLDL, and LDL. To better understand the role of apo B in the body, we have used gene targeting in embryonic stem cells to create a null apo B allele in the mouse. Homozygous apo B deficiency led to embryonic lethality, with resorption of all embryos by gestational day 9. ...

Movies of quiescent prominences seen in H/sub A/ show apparent downflow of matter. The effect is confirmed by observations of downflows in the Ca II K line from large filaments/prominences. Several recent studies show evidence for the opposite case from observations in H/sub A/, i.e., that ...

In this study, we investigated the role of positively and negatively charged amino acids within the 89-99 region of apolipoprotein A-I (apoA-I), which are highly conserved in mammals, on plasma lipid homeostasis and the biogenesis of HDL. We previously showed that deletion of the 89-99 region of apoA-I increased plasma cholesterol and ...

Long-term consumption of artificial sweeteners (AS) has been the recent focus of safety concerns. However, the potential risk of the AS in cardiovascular disease and lipoprotein metabolism has not been investigated sufficiently. We compared the influence of AS (aspartame, acesulfame K, and saccharin) and fructose in terms of functional and structural correlations of apolipoprotein (apo) ...

... N [A] = I [Ql ktk ... 3. TESTING & ANALYSIS ... In most approximation routines it is necessary to show that the computer code is con- verging to a single ...

Apolipoprotein A-I (apoA-I), the main protein component of high density lipoproteins (HDL), is well recognized for its anti-atherogenic, anti-oxidant, and anti-inflammatory properties. Here we report a novel role for apoA-I as a host defence molecule that contributes to the complement-mediated killing of an important gastrointestinal pathogen, ...

High denisty apolipoprotein A-1 (apoLp A-I) has been prepared in a chromatographically and immunochemically homogeneous form. This apoprotein forms trimeric and tetrameric aggregates in aqueous solutions at higher concentrations. ApoLp A-I has been recombined in almost quantitative yield in the presence of lysolecithin with phosphatidylcholine and ...

The sequences of the plasma apolipoproteins have a high degree of internal homology as they contain several 22-mer internal repeats. These amphipathic helical repeats are considered as the structural and functional units of this class of proteins. We proposed that the 22-mer repeats of the plasma apolipoproteins consist of 17-mer helical segments separated by extended ...

The existing well configuration and piping layout are presented in Figures A-i through A-3. Figure A-i shows the SVE pumps and well manifold connections. Figure A-2 shows the Site S wells and their respective manifolds and connection points to the air-wat...

An interrelationship between primary and secondary metabolites of a toxigenic mould (Aspergillus parasiticus var. globosus IMI 120920) was obtained in liquid broth containing various concentrations of dithane Z-78 and propoxur. The results show that a stimulatory response was recorded in gibberellin, protein, sterol and aflatoxin yield in cultures treated with 15, 30, 50 and ...

are associated with HDL, apolipoproteins A-I (apo A-I) and A-II (apo A-II) account for most of their protein content in a 2:1 molar ratio (7). Apo A-I has a clearly defined role in human RCT, while that of apo A-II is unclear (2, 8). Nevertheless, studies in genetically modified mice show a positive correlation ...

Losses of pesticide active ingredients (a.i.) into the atmosphere can occur through several pathways. A main pathway is evaporation drift. The evaporation process of pesticide a.i., after application, is affected by three main factors: Physicochemical properties of the pesticide a.i., weather conditions and crop ...

When restricted to cost arrays possessing the sum Monge property, many combinatorial optimization problems with sum objective functions become significantly easier to solve. Examples include the usual sum-objective-function versions of the assignment problem, the transportation problem, the traveling-salesman problem, and several shortest-path problems. Furthermore, the more ...

AimTo analyze apolipoprotein gene polymorphisms in the Tunisian population and to check the relation of these polymorphisms and homocysteine, lipid and apolipoprotein levels to the coronary artery disease (CAD).MethodsIn healthy blood donors and in patients with CAD complicated by myocardial infarction (MI) four apolipoprotein gene polymorphisms [APO (a) PNR, APO E, APO CI and APO CII] were ...

While low apolipoprotein A-I (apoA-I) levels are primarily associated with increased high density lipoprotein (HDL) fractional catabolic rate (FCR), the factors that regulate the clearance of HDL from the plasma are unclear. In this study, the effect of lipid composition of reconstituted HDL particles (LpA-I) on their rate of clearance ...

bars are ±2. The orange dashed line shows the best-fit model spectrum. The colored curves show emission spectra for the constituent species, scaled by the ratio B[Tdust(a)i]/...

Apolipoprotein A-I plays a central role in the solution structure of high-density lipoproteins. Determining the stoichiometry of lipid-bound apo A-I in the hydrated state is therefore fundamental to understanding how high-density lipoproteins form and function. Here, we use the quantum optical phenomenon of photon antibunching to determine the number of ...

Carriers of the apolipoprotein A-I_Milano (apoA-I_M) variant, R173C, have reduced levels of plasma HDL but no increase in cardiovascular disease. Despite intensive study, it is not clear whether the removal of the arginine or the introduction of the cysteine is responsible for this altered functionality. We ...

High density lipoproteins (HDL) and apolipoprotein A-I (apoA-I) must leave the circulation and pass the endothelium to exert their atheroprotective actions in the arterial wall. We previously demonstrated that the transendothelial transport of apoA-I involves ATP-binding cassette transporter (ABC) A1 and re-secretion of lipidated particles. ...

Electron impact excitation of auroral spectral features in the visible and ultraviolet are computed by solving the complete electron transport equation. Excitation rates are given for several bands of N/sub 2/ (A /sup 3//Sigma/, B /sup 3//Pi/, W /sup 3//Delta/, a /sup 1//Pi/, C /sup 3//Pi/) and of N/sub 2//sup +/, for bands of O/sub 2/ (a /sup 1//Delta/, b /sup 1//Sigma/) and ...

Apolipoprotein A-I (ApoA-I), a major component of HDL, binds haptoglobin, a plasma protein transporting to liver or macrophages free Hb for preventing hydroxyl radical production. This work aimed to assess whether haptoglobin protects ApoA-I against this radical. Human ApoA-I structure, as analyzed by ...

Two-dimensional electrophoretic analysis of plasma lipoproteins from male Osborne-Mendel rats consistently reveals three isoforms of apolipoprotein A-I (apo-A-I) with the following apparent pI values and quantitative distribution: isoform 3, pI = 5.68, 69%; isoform 4, pI = 5.55, 29%; isoform 5, pI = 5.44, 2%. The two major isoforms were obtained by ...

Uncontrolled storm runoff in rapidly growing urban areas of the tropics is severely constraining land development, reducing groundwater reserves, and hastening soil erosion. This manual provides practical guidelines and technical materials to assist A.I.D...

severe drop in local wall temperature during bubble growth appears to ... Estimates of the heat transfer rate during bubble growth were presented, in ...

...grazing losses. (a) A grazing loss due to drought is eligible for LFP only if the grazing...pastureland for the county, rated by the U.S. Drought Monitor as having a: (i) D2 (severe drought) intensity in any area of the...

Substrates for matrix metalloproteinase (MMP)-14 were previously identified in human plasma using proteomic techniques. One putative MMP-14 substrate was apolipoprotein A-I (apoA-I), a major component of high-density lipoprotein (HDL). In vitro cleavage assays showed that lipid-free apoA-I is a more accessible ...

The peptide 4F is known to have potent anti-atherogenic activity. 4F is an 18 residue peptide that has a sequence capable of forming a class A amphipathic helix. Several other class A amphipathic helical, 18 residue peptides with the same polar face but with increasing Phe residues on the nonpolar face have been synthesized with varying degrees of biological activity. In this ...

A series of field trials were carried out from 2000 to 2003. Neonicotinoid insecticides applied as seed potato treatments at planting were tested to control wireworms in potato crops. Compounds were applied as drench or spray. Neonicotinoids tested were imidacloprid at rates of 35, 70, 88, and 175 g a.i.; thiamethoxam at 17.5, 35, 50, 70, and 140 g a.i.; ...

I/UCRC Fact Sheets high-power flared cavity surface emitting semiconductor laser. "The figure shows a schematic of a high-power flared cavity surface emitting semiconductor laser....

Positive Negative Outcome ExpectationsCircle one number on each line to show if you agree or disagree with the following statements about cigarette smoking. Strongly Agree Agree Disagree Strongly Disagree a) I think I would enjoy smoking. 1 2 3 4 b)

Jul 31, 2002 ... Illustration shows Hastelloy-X lower manifold, air supply, 1-1/2-in. expansion loops, table, free-jet airflow, upper plenum, A/I/S section, ...

... Dropping of liquid fuel in drums by parachutg A. & iL.. ... Sunmn :Y The tests carried out show that liquid fuel in 40/50 gal. ...

New treatment approaches are needed for patients with asthma. Apolipoprotein A-I (apoA-I), the major structural protein of high-density lipoproteins, mediates reverse cholesterol transport and has atheroprotective and anti-inflammatory effects. In this study, we hypothesized that an apoA-I mimetic peptide might be effective at inhibiting asthmatic airway ...

The cathode phenomena during the conduction phase of a pseudospark discharge are investigated with different cathode materials: Cu, Mo, Ni, Ta, and W/Re. The discharge gas was hydrogen with a gas pressure of 40 Pa. At a pulse duration of 2.7 microsecond and a maximum current of 9 kA, i.e., at a transferred charge of 11 mC/discharge, mass erosion rates of ...

Copper deficiency in rats produces a hypercholesterolemia with a marked increase in HDL fraction. This study investigated changes in the plasma distribution and composition of HDL subclasses as affected by copper deficiency. Plasma HDL were separated into the following three subclasses by heparin-affinity chromatography: HDL containing no apo E but high in apo A-I (HDL-E0); ...

Apolipoprotein A-I (ApoA-I)/high-density lipoprotein (HDL)-raising treatments are effective antiatherosclerotic strategies. We have compared the antiatherogenic effects of human ApoA-I (hApoA-I) overexpression by intraportal and intramuscular gene transfer in atherosclerotic ApoE-knockout mice. Atherosclerotic ...

The crystal structures of the Ca(2+)- and H(+)-ATPases shed light into the membrane embedded domains involved in binding and ion translocation. Consistent with site-directed mutagenesis, these structures provided additional evidence that membrane-spanning segments M4, M5, M6 and M8 are the core through which cations are pumped. In the present study, we have used alanine/serine scanning mutagenesis ...

IntroductionVisceral leishmaniasis is a parasitic infection caused by Lesihmania donovani complex and transmitted by the bite of the phlebotomine sand fly. It is an endemic disease in many developing countries with more than 90% of the cases occurring in Bangladesh, India, Nepal, Sudan, Ethiopia and Brazil. The disease is fatal if untreated. The disease is conventionally diagnosed by demonstrating ...

Hydrogen peroxides are commonly used in greenhouses for cleaning purposes and disinfection of irrigation water systems, i.e., to prevent clogging by duckweed (Lemna minor), algae and other (micro)organisms. This use contains a potential risk of involuntary contact to the plants, e.g., to roots through irrigation or to the plant leaves through accidental droplets (spraying mist). To help growers to ...

Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particles similar to nascent high-density lipoproteins, which reduce the risk of coronary artery disease. The major protein component of high-density lipoproteins is human apolipoprotein A-I, and the corresponding protein component of Nanodiscs is membrane scaffold protein 1 (MSP1), a 200-residue ...

Several HDL binding proteins, quite disparate in structure, have recently been cloned and their role in HDL metabolism is currently being assessed. High density lipoprotein binding protein, HBP (vigilin), which lacks a transmembrane domain is responsive to cell cholesterol levels, but its physiological significance remains unknown. On the other hand much is known about SR-B1, ...

Apolipoprotein E (apoE) is associated with several classes of plasma lipoproteins and mediates uptake of lipoproteins through its ability to interact with specific cell surface receptors. Besides its role in cardiovascular diseases, accumulating evidence has suggested that apoE could play a role in neurodegenerative diseases, such as Alzheimer disease. In vertebrates, ...

BackgroundHereditary amyloidosis due to mutations of apolipoprotein A-I (apoA-I) is a rare disease characterized by the deposition of amyloid fibrils constituted by the N-terminal fragment of apoA-I in several organs. L75P is a variant of apoA-I associated with systemic amyloidosis predominantly involving the liver, kidneys, and ...

Hepatic steatosis is considered to have an important impact on liver tumorigenesis, despite a lack of clear experimental evidence. Histopathological analysis of H-ras12V transgenic mice showed liver lesions on a steatosis background had significantly higher incidence than on a non-steatosis background. Further investigation showed that apolipoprotein ...

2-Dimethylaminomethylene-1-benzosuberone 1 was coupled with diazotized aniline derivatives to afford a series of the hitherto unreported 2-arylazo-1-benzosuberones 3a-i. The tautomeric structure and the effect of substituents on the tautomeric form (s) of the products 3a-i were discussed. Similar coupling of the enaminone 1 with diazonium salts of ...

ORP1L is an oxysterol binding homologue that regulates late endosome (LE) positioning. We show that ORP1L binds several oxysterols and cholesterol, and characterize a mutant, ORP1L ?560-563, defective in oxysterol binding. While wild-type ORP1L clusters LE, ORP1L ?560-563 induces LE scattering, which is reversed by disruption of the endoplasmic reticulum ...

The electrical transport in multiwalled carbon nanotubes is shown to be ballistic at room temperature with mean free paths on the order of tens of microns. The measurements are performed both in air and in the transmission electron microscope by contacting the free end of a nanotube pointing out of a fiber to a liquid metal and measuring the dependence of the nanotube resistance between the ...

According to the concepts of attribute axis and attribute coordinate system, porphine and 11 kinds of bridge carbon substituted porphyrins as donors and 9 common acceptors A-I have been designed and calculated at the density functional B3LYP level. The substituent effects on the molecular orbital energy levels of the porphyrin derivatives have been discussed and promising ...

Apolipoprotein A-I (apoA-I) stabilizes anti-atherogenic high density lipoprotein particles (HDL) in the circulation and governs their biogenesis, metabolism, and functional interactions. To decipher these important structure�function relationships, it will be necessary to understand the structure, stability, and plasticity of the apoA-I molecule. ...

SUMMARYHigh-density lipoproteins (HDL) are complexes of proteins (mainly apoA-I and apoA-II) and lipids that remove cholesterol and prevent atherosclerosis. Understanding distinct properties of the heterogeneous HDL population may help develop new diagnostic tools and therapies for atherosclerosis. Mature human HDL form two major subclasses differing in particle diameter and ...

Whereas hepatocytes secrete the major human plasma high density lipoproteins (HDL)-protein, apo A-I, as lipid-free and lipidated species, the biogenic itineraries of apo A-II and apo E are unknown. Human plasma and HepG2 cell-derived apo A-II and apo E occur as monomers, homodimers and heterodimers. Dimerization of apo A-II, which is more lipophilic than apo ...

Self-association is an inherent property of the lipid-free forms of several exchangeable apolipoproteins, including apolipoprotein A-I (apoA-I), the main protein component of high density lipoproteins (HDL) and an established antiatherogenic factor. Monomeric lipid-free apoA-I is believed to be the biologically ...

One type of RNA editing converts adenosines to inosines (A?I editing) in double-stranded RNA (dsRNA) substrates. A?I RNA editing is mediated by adenosine deaminase acting on RNA (ADAR) enzymes. A?I RNA editing of protein-coding sequences of a limited number of mammalian genes results in recoding and subsequent alterations of their ...

BackgroundStatins are first-line pharmacotherapeutic agents for hypercholesterolemia treatment in humans. However the effects of statins on atherosclerosis in mouse models are very paradoxical. In this work, we wanted to evaluate the effects of simvastatin on serum cholesterol, atherogenesis, and the expression of several factors playing important roles in reverse cholesterol ...

Background and purpose: Ro 11-1464 is a thienotriazolodiazepine previously described to selectively stimulate apolipoprotein A-I (apoA-I) production and mRNA level in human liver cells. Here we studied its effects upon oral administration to human apoA-I transgenic (hapoA-I) mice. Experimental approach: ...

The apoA-I molecule adopts a two-domain tertiary structure and the properties of these domains modulate the ability to form HDL particles. Thus, human apoA-I differs from mouse apoA-I in that it can form smaller HDL particles; the C-terminal ?-helix is important in this process and human apoA-I is unusual in containing aromatic amino ...

Background: While much has been written in the evaluation literature on the theory of evaluations and on specific cases, there is still no comprehensive and easy to use indicator that can be used to hold organizations to the principles of effective evaluation, to score their quality in several areas, and to offer an immediate diagnostic for improvements. Purpose: The article ...

Several parameters and risk factors were compared between Korean male myocardial infarction (MI) patients (n = 10) and angina pectoris (AP) patients (n = 17) to search unique biomarkers for myocardial infarction (MI) in lipoprotein level. Individual serum and lipoprotein fractions (VLDL, LDL, HDL₂, HDL₃) were isolated and analyzed ...

Analysis of high density lipoprotein (HDL) isolated from serum without major hypertriglyceridaemia and by five different precipitation methods showed that there were no significant differences in total cholesterol and triglyceride concentrations in the HDL supernatants prepared by the different methods but that free cholesterol, phospholipid, apolipoprotein (apo) ...

Amyloidoses constitute a group of diseases in which soluble proteins aggregate and deposit extracellularly in tissues. Nonhereditary apolipoprotein A-I (apoA-I) amyloid is characterized by deposits of nonvariant protein in atherosclerotic arteries. Despite being common, little is known about the pathogenesis and significance of apoA-I deposition. In this ...

Amyloidoses constitute a group of diseases in which soluble proteins aggregate and deposit extracellularly in tissues. Nonhereditary apolipoprotein A-I (apoA-I) amyloid is characterized by deposits of nonvariant protein in atherosclerotic arteries. Despite being common, little is known about the pathogenesis and significance of apoA-I deposition. In this ...

Poly(butyl cyanoacrylate) nanoparticles coated with poloxamer 188 (Pluronic) F68) and also, as shown previously, polysorbate 80 (Tween 80) considerably enhance the anti-tumour effect of doxorubicin against an intracranial glioblastoma in rats. The investigation of plasma protein adsorption on the surface of the drug-loaded nanoparticles by two-dimensional electrophoresis (2-D PAGE) revealed that ...

In this study, we propose a structure for the heterodimer between apolipoprotein A-I_Milano and apolipoprotein A-II (apoA-I_M�apoA-II) in a synthetic high-density lipoprotein (HDL) containing L-?-palmitoyloleoyl phosphatidylcholine. We applied bioinformatics/computational tools and procedures, such as molecular ...

High levels of HDL-cholesterol (HDL-C) protect against coronary heart disease susceptibility, but the metabolic mechanisms underlying elevated HDL-C levels are poorly understood. We now report the turnover of isologous radioiodinated HDL apolipoproteins, apo A-I and apo A-II, in 15 female subjects on a metabolic diet with HDL-C levels ranging from 51 to 122 mg/dl. The ...

Essential fatty acids (EFA) are important for the normal development and functioning of the brain, retina and immune competent cells. Severely neurologically handicapped children often have feeding difficulties, and the composition of the diet may be critical with respect to an optimal nutrient content. The aim of the present investigation was to evaluate if the dietary ...

Lecithin cholesterol acyl transferase (LCAT) deficiency is associated with low high-density lipoprotein (HDL) and the presence of an abnormal lipoprotein called lipoprotein X (Lp-X) that contributes to end-stage renal disease. We examined the possibility of using LCAT an as enzyme replacement therapy agent by testing the infusion of human recombinant (r)LCAT into several mouse ...

Cestodes are unable to synthesize de novo most of their own membrane lipids, including cholesterol, and have to take them up from the host during an infection. The underlying molecular mechanisms are so far unknown. Here we report the identification and characterization of a novel gene, Emabp, which is expressed by larval stages and adults of the fox tapeworm Echinococcus multilocularis. The ...

Cestodes are unable to synthesize de novo most of their own membrane lipids, including cholesterol, and have to take them up from the host during an infection. The underlying molecular mechanisms are so far unknown. Here we report the identification and characterization of a novel gene, Emabp, which is expressed by larval stages and adults of the fox tapeworm Echinococcus multilocularis. The ...

Apolipoprotein A-I (apoA-I) Nichinan, a naturally occurring variant with ?E235 in the C terminus, is associated with low plasma HDL levels. Here, we investigated the tertiary structure, lipid-binding properties, and ability to induce cellular cholesterol efflux of apoA-I Nichinan and its C-terminal peptide. Thermal and chemical denaturation experiments ...

The molecular genetic defect of a female patient with apolipoprotein A-I (apoA-I) deficiency and premature atherosclerosis was examined. Her parents were first cousins. Her plasma density fraction from 1.063 to 1.21 g/ml contained no apoA-I on SDS/PAGE and no measurable high density lipoprotein cholesterol. Southern blot hybridization ...

Apolipoprotein A-II (apoA-II) is the second major apolipoprotein following apolipoprotein A-I (apoA-I) in HDL. ApoA-II has multiple physiological functions and can form senile amyloid fibrils (AApoAII) in mice. Most circulating apoA-II is present in lipoprotein A-I/A-II. To study the influence of apoA-I on apoA-II and AApoAII ...

Nine linear C23 and C21 acetogenins, named cananginones A-I (1-9), were isolated from stem bark of Cananga latifolia. Their structures were established by spectroscopic methods. These compounds showed cytotoxicity against three cancer cell lines (KB, MCF7 and NCI-H187) with IC(50) values in the range 16.6-129.7?M. Only 5 showed weak ...

Under iron-limiting conditions, Pseudomonas aeruginosa PAO1 secretes a fluorescent siderophore called pyoverdine (Pvd). After chelating iron, this ferric siderophore is transported back into the cells via the outer membrane receptor FpvA. The Pvd-dependent iron uptake pathway requires several essential genes involved in both the synthesis of Pvd and the uptake of ferric Pvd ...

Cardiovascular disease (CVD) results from complex interactions between genetic and environmental factors. The evidence supports that gene-environment interactions modulate plasma lipid concentrations and potentially CVD risk. Several genes (eg, apolipoprotein A-I and A-IV, apolipoprotein E, and he...

The measurement of benefits from export promotion programs raises difficult practical and technical issues. This paper explores methodologies for ex post impact analysis of export promotion projects. The paper discusses several types of benefits: direct b...

Over the past several years, A.I.D. has carried out numerous projects to combat desertification, i.e., to improve the management of dryland resources such as soils, forests, and range and water resources. Many of these projects were designed as a result o...

The effects of different acylglycerides on the conformation and charge of apolipoprotein A-I (apoA-I) have been investigated in reconstituted high density lipoproteins (LpA-I). Various amounts of diacylglycerol (DG) and triacylglycerol (TG) were incorporated into sonicated spherical LpA-I particles containing 2 ...

Although statin treatment leads consistently to a reduction in major adverse coronary events and death in clinical trials, approximately 60 to 70% residual risk of these outcomes still remains. One frontier of investigational drug research is treatment to increase HDL, the �good cholesterol� that is associated with a reduced risk of coronary artery disease. HDL and its major protein ...

Food webs can be regarded as energy transporting networks in which the weight of each edge denotes the energy flux between two species. By investigating 21 empirical weighted food webs as energy flow networks, we found several ubiquitous scaling behaviors. Two random variables A(i) and C(i) defined for each vertex i, representing the total flux (also ...

In this study a large family group affectd with Tangier disease has been investigated. Besides two homozygous propositi, several heterozygous patients have been identified on the basis of quantitative measurements of high density lipoproteins and their constitutive polypeptides. By a variety of quantitative immunological methods, such as one-dimensional Laurell eletrophoresis, ...

Several clinical and angiographic intervention trials have shown that fibrate treatment leads to a reduction of the coronary events associated to atherosclerosis. Fibrates are ligands for peroxisome proliferator-activated receptor alpha (PPARalpha) that modulate risk factors related to atherosclerosis by acting at both systemic and vascular levels. Here, we investigated the ...

Our aim was to characterize HDL subspecies and fat-soluble vitamin levels in a kindred with familial apolipoprotein A-I (apoA-I) deficiency. Sequencing of the APOA1 gene revealed a nonsense mutation at codon -2, Q[-2]X, with two documented homozygotes, eight heterozygotes, and two normal subjects in the kindred. Homozygotes presented markedly decreased HDL ...

The nuclear magnetic resonance (NMR) technique of chemically induced dynamic nuclear polarization (CIDNP) has been used to study the reactions between photoexcited flavins and a wide variety of nucleotides, dinucleotides, and oligonucleotides. The greatest emphasis is placed on the purine nucleosides adenosine (A), inosine (I), and guanosine (G), particularly guanosine. The presence of G ...

organizations. Within this demo we show a way of evaluating perfor- mance of AMQP middleware by using an adapted critical deployments, e.g., JPMorgan reported an AMQP environ- ment supporting 2,000 users on five MRG n/a iMatix Corporation OpenAMQ 0-9-1 Apache Software Foundation Qpid 0-10 Rabbit Technologies Ltd

update step in the modeling process. We show that by using sparse matrix methods, a direct-iterative = v where A is sparse and symmetric positive definite. Iterative methods such as Lanczos and Jacobi iteration. Ideally, inverse iteration requires only repeated linear system solutions of the form (A - i

A semi-empirical model of spectrum of X-ray tube was developed for the radiotherapy facility at the A. I. Burnazyan Federal Medical Biophysical Center for radiobiological experiments. The spectrum calculated by a programme EGSnrc was compared with the spectrum of the model. The results of the spectrum computations showed a good concord ...

Measurements on noise in space-charge-limited solid-state diodes and triodes show that the limiting noise of these devices is thermal noise. The equivalent noise resistance R sub n of the diodes is equal to the d.c. resistance V sub a/I sub a of the devic...

Circle one number on each line to show if you agree or disagree with the following statements about cigarette smoking. Strongly Strongly Agree Agree Disagree Disagree a) I think I would enjoy smoking. 1 2 3 4 b) I think smoking would give me something

and a model of the lactose operon regulation in E.coli. Keywords: Calculus of Looping Sequences, Maude, model into the latter. Section 4 shows the analysis of some examples and of a model of the lactose operon regulation a RRNA Polime- rase NO TRANSCRIPTION a) i p o z y a R RNA Polime- rase b) LACTOSE lac Repressor R beta

Apolipoprotein A-I (ApoA-I) is a protein implicated in the solubilization of lipids and cholesterol from cellular membranes. The study of ApoA-I in phospholipid (PL) monolayers brings relevant information about ApoA-I/PL interactions. We investigated the influence of PL charge and acyl chain organization on the interaction with ApoA-I ...

Two cysteine endopeptidases from latex of Araujia angustifolia (araujiain aI and araujiain aIII) were purified and characterized by means of conventional and proteomics techniques (MALDI-TOF). N-terminal sequences showed a high percentage of identity with cysteine proteinases belonging to the papain family. The peptide mass fingerprint analysis ...

Some 212 A.I.D. evaluation reports for FY85 and FY86, 50 of them analyzed in-depth, provided the basis for the study of the sustainability of the institutional components of A.I.D. projects. The study showed that only 11% of the projects received highly p...

the development and propagation of easterly wave disturbances over West Africa. Perturbations are started from over West Africa (Burpee 1972, Rennick 1981, Newell and Kidson 1979, 1984). Grist et al. (2002) further analysis of the basic states further showed that for West Africa as a whole baroclinic and barotropic

and show that they all fall short of the task due to lack of story knowledge and structure i.e. a lack 11A Model of Story Generation A thesis submitted to the University of Manchester for the degree of M Introduction 11 1.1 Existing A.I. story generators : : : : : : : : : : : : : : : : : : : : 13 1.2 Story

We report the in vitro efficacy of recombinant LCAT produced by lcat gene-transduced proliferative adipocytes (ccdPA/lcat), which has been developed for enzyme replacement therapy. ApoA-I-specific immunodetection in combination with 1D and 2D gel electrophoreses showed that the disturbed high-density lipoprotein subpopulation profile was clearly ...