Division of Intramural
Research
Laboratory of Molecular Cardiology
Robert S. Adelstein, M.D.
Chief, Laboratory of Molecular Cardiology
and Muscle Molecular Biology Section
Phone: (301) 496-1865
Fax: (301) 402-1542
Email: adelster@nhlbi.nih.gov
Research Interests
The Section on Muscle Molecular Biology studies the function
of vertebrate nonmuscle myosin II, including II-A, II-B and
II-C. Our recent work focuses on the effects of ablating,
mutating and decreasing the amount of nonmuscle myosin II-B
and II-A in mice. We have found that ablation, the introduction
of a single amino acid mutation, as well as reduction of the
II-B gene product leads to defects in the mouse heart and brain
including a reduction in cardiac myocytes and hydrocephalus.
Presently, we are studying the mechanisms that are responsible
for these pathological changes. We are also looking at
the effect of ablating nonmuscle myosin II-A on mouse development
and on cellular processes. These knockout mice die as
early as E6.0.
Recent Publications
- Wei,
Q. and Adelstein, R.S.: Pitx2a expression alters
actin-myosin cytoskeleton and migration of HeLa cells through
Rho GTPase signaling. Mol. Biol. Cell 13:
683-697, 2002.
- Bridgman,
P.C., Dave, S., Asnes, C.F., Tullio, A.N. and Adelstein,
R.S.: Myosin IIB is required for growth cone motility.
J. Neuroscience21: 6159-6169, 2002.
- Latham,
Jr., V.M., Yu, E.H.S., Tullio, A.N., Adelstein, R.S. and
Singer, R.H.: A Rho-dependent signaling pathway
operating through myosin localizes Beta-actin mRNA in fibroblasts.
Curr. Biol. 11: 1010-1016, 2001.
- Tullio,
A.N., Bridgman, P.C., Tresser, N.J., Chan, C.-C., Conti,
M.A., Adelstein, R.S. and Hara, Y.: Structural
abnormalities develop in the brain after ablation of the
gene encoding nonmuscle myosin II-B heavy chain. J.
Comparative Neurol. 433: 62-74, 2001.
- Buxton,
D.B. and Adelstein, R.S.: Calcium-dependent threonine
phosphorylation of nonmuscle myosin in stimulated RBL-2H3
cells. J. Biol. Chem.275: 34772-34779,
2000.
- Wei,
Q. and Adelstein, R.S.: Conditional expression
of a truncated fragment of nonmuscle myosin II-A alters
cell shape but not cytokinesis in HeLa cells. Mol.
Biol. Cell 11: 3617-3627, 2000.
- Takeda,
K, Yu, Z-X, Qian, S, Chin, T K, Adelstein, R S and Ferrans,
V J : Nonmuscle myosin II localizes to the Z-lines and
intercalated discs of cardiac muscle and the Z-lines of
skeletal muscle. Cell Motil. Cytoskel.46:
59-68, 2000.
- Uren,
D., Hwang, H.K., Hara, Y., Takeda, K., Kawamoto, S., Tullio,
A.N., Yu, Z.-X., Ferrans, V.J., Tresser, N., Grinberg, A.,
Preston, Y.A. and Adelstein, R.S.: Gene dosage
affects the cardiac and brain phenotype in nonmuscle myosin
II-B-depleted mice. J. Clin. Invest. 105:
663-671, 2000.
- Bhatia-Dey,
N., Taira, M., Conti, M.A., Nooruddin, H. and Adelstein,
R.S.: Differential expression of nonmuscle myosin
heavy chain genes during Xenopus embryogenesis.
Mech. Dev. 78: 33-36, 1998.
- Tullio,
A.N., Accili, D., Ferrans, V.J., Yu, Z.-X., Takeda, K.,
Grinberg, A., Westphal, H., Preston, Y.A. and Adelstein,
R.S.: Nonmuscle myosin II-B is required for normal
development of the mouse heart. Proc. Natl. Acad.
Sci. USA 94: 12407-12412, 1997.
- Kelley,
C.A., Sellers, J.R., Gard, D.L., Bui, D., Adelstein, R.S.
and Baines, I.C.: Xenopus nonmuscle myosin heavy
chain isoforms have different subcellular localizations
and enzymatic activities. J. Cell Biol. 134:
675-687, 1996.
- Liu,
P.P., Wijmenga, C., Hajra, A., Blake, T.B., Kelley, C.A.,
Adelstein, R.S., Bagg, A., Rector, J., Cotelingam, J., Willman,
C.L. and Collins, F.S.: Identification of the
chimeric protein product of the CBFB-MYHII fusion gene in
Inv(16) leukemia cells. Genes, Chrom. & Cancer16:
77-87, 1996.
- Pato,
M.D., Sellers, J.R., Preston, Y.A., Harvey, E.V. and Adelstein,
R.S.: Baculovirus expression of chicken nonmuscle
heavy meromyosin II-B: Characterization of alternatively
spliced isoforms. J. Biol. Chem.271:
2689-2695, 1996.
Members
Jianjun Bao, Ph.D.
Myung-Chul Chung, Ph.D.
Mary Anne Conti, Ph.D.
Sachiyo Kawamoto, M.D., Ph.D.
Hiroko Kishi, M.D., Ph.D.
XueFei Ma, Ph.D.
Cathy Magruder, Office Manager
Yvette A. Preston, B.S.
Antoine F. Smith
Kazuyo Takeda, M.D., Ph.D.
QiZe Wei, Ph.D.
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Department of Health
and Human Services
National
Institutes of Health
National Heart, Lung, and Blood
Institute