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Requested Publication

Title: Effect of carbohydrate and nitrogen on hydrogen peroxide formation by wood decay fungi in solid medium

Source: Elsevier

Author(s): Highley, Terry L.

Publication Year: 1987  View PDF » Mail this web page's link »

Publication Series: Journal Articles

Abstract: Hydrogen peroxide (H2O2) has been implicated in degradation of wood by both brown-rot and white-rot fungi. This study found that low concentrations of nitrogen and carbohydrates (cellobiose, glucose, xylose and mannose) in an agar medium had little effect on H2O2 production by white-rot fungi. However, low concentrations of nitrogen and carbohydrates stimulated H2O2 production by brown-rot fungi. Use of the chromogen 2,2'-azino-di(3-ethyl benzthiazoline-6-sulphonic acid) (ABTS) with horseradish peroxidase to detect H2O2 by the fungi was slightly better than detection by the chromogen o-dianisidine with horseradish peroxidase. An auxiliary test to check the role of H2O2 in wood decay found that hydrogen peroxide-negative isolates of the white-rot fungi Phanerochaete chrysosporium and Ganoderma applanatum were unable to decay sweetgum and southern pine.

Keywords: Wood decay, fungi, hydrogen peroxide

File size: 144 kb(s)

Date posted: 05/07/2012
RITS Product ID: 42016

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