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Enzyme-Linked Immunosorbent Assay Compared with Gas Chromatography/Mass Spectrometry for the Determination of Triazine Herbicides in WaterBy E.M. Thurman, Michael Meyer, Michael Pomes, C.A. Perry, and A.P. SchwabAbstractAn enzyme-linked immunosorbent assay (ELISA) was compared to a gas chromatography/mass spectrometry (GC/MS) procedure for the analysis of triazine herbicides and their metabolites in surface water and groundwater. Apparent recoveries from natural water and spiked water by both methods were comparable at 0.2-2 Îg/L. Solid-phase extraction (SPE) was examined also, and recoveries were determined for a suite of triazine herbicides. A significant correlation was obtained between the ELISA and GC/MS method for natural water samples that were extracted by SPE. Because ELISA was developed with an atrazine-like compound as the hapten with conjugation at the 2-position, it was selective for triazines that contained both ethyl and isopropyl side chains. Concentrations for 50% inhibition (IC50) were as follows: atrazine, 0.4 Îg/L; ametryne, 0.45 Îg/L; prometryn and propazine, 0.5 Îg/L; prometon, 0.7 Îg/L; simazine and terbutryn, 2.5 Îg/L; hydroxyatrazine, 28 Îg/L; deethylatrazine and deisopropylatrazine, 30 Îg/L; cyanazine, 40 Îg/L; didealkylatrazine had no response. The combination of screening analysis by ELISA, which requires no sample preparation and works on 160 ÎL of sample, and confirmation by GC/MS was designed for rapid, inexpensive analysis of triazine herbicides in water. Additional information about the Organic Geochemistry Research Laboratory can be found at: http://ks.water.usgs.gov/studies/reslab/Thurman, E.M., Meyer, M., Pomes, M., Perry, C.A., and Schwab, A.P., 1990, Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water: Analytical Chemistry, v. 62, no. 18, p. 2043-2048. To request a paper copy of this journal article, email: scribner@usgs.gov |
U.S. Department of the Interior |
U.S. Geological Survey
URL: /pubs/abstracts/emt.09-90.html
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The USGS has collected hydrologic information in Kansas since 1895 with the first USGS Cooperative
Water Program funding agreement for 6 streamflow gages with the Kansas Water Board. USGS collects streamflow
and gage-height data; reservoir content; water-quality and water-quantity data; suspended-sediment data;
and groundwater levels. Hydrologic studies are conducted on national, regional, statewide, and local levels.
The USGS in Kansas works cooperatively with 31 Federal, State, and local agencies, such as the Kansas Water Office, the U.S.Army Corps of Engineers, and the City of Wichita