Commercially Available STR Multiplex Kits
Kits from commercial sources are now
predominantly used by labs around the world for their ease of use
and high discriminatory power.
For further
information, see Butler, J.M. (2006) Genetics and genomics of core STR loci used
in human identity testing.
J. Forensic Sci. 51(2): 253-265.
[Explanation
of STR Multiplexes] [STR Fact Sheets]
STR Kits from Applied Biosystems
(Foster City, CA)
- AmpFlSTR
Identifiler:
D8S1179,
D21S11, D7S820, CSF1PO, D3S1358,
TH01,
D13S317,
D16S539, D2S1338,
D19S433, VWA,
TPOX, D18S51,
Amelogenin, D5S818, FGA
- AmpFlSTR
Identifiler
Direct:
D8S1179,
D21S11, D7S820, CSF1PO, D3S1358,
TH01,
D13S317,
D16S539, D2S1338,
D19S433, VWA,
TPOX, D18S51,
Amelogenin, D5S818, FGA
- AmpFlSTR
Identifiler
Plus:
D8S1179,
D21S11, D7S820, CSF1PO, D3S1358,
TH01,
D13S317,
D16S539, D2S1338,
D19S433, VWA,
TPOX, D18S51,
Amelogenin, D5S818, FGA
- AmpFlSTR NGM:
D10S1248, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, D21S11, D18S51,
D22S1045,
D19S433, TH01, FGA,
D2S441,
D3S1358,
D1S1656,
D12S391
- AmpFlSTR NGM SElect:
D10S1248, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, D21S11, D18S51,
D22S1045,
D19S433, TH01, FGA,
D2S441,
D3S1358,
D1S1656,
D12S391, SE33
- AmpFlSTR GlobalFiler:
D3S1358, VWA,
D16S539, CSF1PO,
TPOX, Yindel,
Amelogenin,D8S1179, D21S11, D18S51, DYS391, D2S441,
D19S433, TH01, FGA,
D22S1045, D5S818,
D13S317, D7S820,
SE33, D10S1248,
D1S1656,
D12S391, D2S1338
- AmpFlSTR VeriFiler:
D10S1248,
D1S1656,
Amelogenin, D2S1338,
D22S1045,
D19S433, TH01,
D2S441, D6S1043,
D12S391
- AmpFlSTR
MiniFiler:
D13S317, D7S820,
Amelogenin, D2S1338,
D21S11,
D16S539, D18S51, CSF1PO, FGA
- AmpFlSTR
Yfiler:
DYS456,
DYS389I,
DYS390,
DYS389II, DYS458, DYS19,
DYS385a/b,
DYS393, DYS391, DYS439,
DYS635,
DYS392, Y_GATA_H4,
DYS437, DYS438, DYS448
- AmpFlSTR
SGM Plus:
D3S1358, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, D21S11, D18S51,
D19S433, TH01, FGA
- AmpFlSTR
Profiler Plus:
D3S1358, VWA, FGA,
Amelogenin,
D8S1179, D21S11, D18S51, D5S818,
D13S317, D7S820
- AmpFlSTR
Profiler Plus ID:
D3S1358, VWA, FGA,
Amelogenin,
D8S1179, D21S11, D18S51, D5S818,
D13S317, D7S820
- AmpFlSTR
COfiler :
D3S1358,
D16S539, Amelogenin,
TH01, TPOX, CSF1PO, D7S820
- AmpFlSTR
Sinofiler (available only in China):
D8S1179,
D21S11, D7S820, CSF1PO, D3S1358, D5S818,
D13S317,
D16S539, D2S1338,
D19S433, VWA,
D12S391, D18S51,
Amelogenin, D6S1043, FGA
- AmpFlSTR
Profiler:
D3S1358, VWA, FGA,
Amelogenin,
TH01, TPOX, CSF1PO, D5S818,
D13S317, D7S820
- AmpFlSTR
SEfiler:
D3S1358, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, SE33, D19S433, TH01, FGA,
D21S11, D18S51
- AmpFlSTR
SEfiler Plus:
D3S1358, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, SE33, D19S433, TH01, FGA,
D21S11, D18S51
- AmpFlSTR Green I :
Amelogenin, TH01, TPOX, CSF1PO
- AmpFlSTR Blue:
D3S1358, VWA, FGA
STR Kits from
Promega Corporation
(Madison, WI)
- PowerPlex 16 :
D3S1358,
TH01, D21S11, D18S51,
Penta E, D5S818,
D13S317, D7S820,
D16S539, CSF1PO,
Penta D,
Amelogenin, VWA,
D8S1179, TPOX, FGA
- PowerPlex Fusion: Amelogenin, D3S1358, D1S1656, D2S441,
D10S1248,
D13S317,
Penta E,
D16S539,
D18S51, D2S1338, CSF1PO,
Penta D,
TH01, VWA, D21S11, D7S820, D5S818, TPOX, DYS391,
D8S1179,
D12S391,
D19S433, FGA,
D22S1045
- PowerPlex Y:
DYS391,
DYS389I, DYS439,
DYS389II, DYS438,
DYS437, DYS19, DYS392,
DYS393, DYS390,
DYS385a/b
- PowerPlex Y23: DYS576,
DYS389I, DYS448,
DYS389II, DYS19, DYS391,
DYS481, DYS549, DYS533, DYS438,
DYS437, DYS570,
DYS635, DYS390, DYS439, DYS392,
DYS643,
DYS393, DYS458,
DYS385a/b,
DYS456, Y_GATA_H4
- PowerPlex 1.2: almost same as
PowerPlex 1.1 but for ABI 310 instead of FMBIO
- PowerPlex 1.1: for FMBIO users
- PowerPlex 2.1: for FMBIO users
- PowerPlex 16 BIO: same as PowerPlex 16
but for FMBIO users
- PowerPlex ES:
D3S1358,
TH01, D21S11, D18S51, SE33,
Amelogenin, VWA,
D8S1179, FGA
- PowerPlex 16
HS:
D3S1358,
TH01, D21S11,
D18S51,
Penta E, D5S818,
D13S317, D7S820,
D16S539, CSF1PO,
Penta D,
Amelogenin, VWA,
D8S1179, TPOX, FGA
- PowerPlex 18D: D3S1358,
TH01, D21S11,
D18S51,
Penta E, D5S818,
D13S317, D7S820,
D16S539,
Amelogenin, VWA,
D8S1179, TPOX, FGA,
D19S433, D2S1338
- PowerPlex ESX 16:
Amelogenin, D3S1358,
TH01, D21S11,
D18S51, D10S1248,
D1S1656, D2S1338,
D16S539, D22S1045,
VWA,
D8S1179, FGA, D2S441,
D12S391,
D19S433
- PowerPlex ESX 17:
Amelogenin, D3S1358,
TH01, D21S11,
D18S51, D10S1248,
D1S1656, D2S1338,
D16S539, D22S1045,
VWA,
D8S1179, FGA, D2S441,
D12S391,
D19S433, SE33
- PowerPlex ESI 16:
Amelogenin, D3S1358,
D19S433, D2S1338,
D22S1045,
D16S539,
D18S51, D1S1656,
D10S1248, D2S441,
TH01, VWA, D21S11,
D12S391,
D8S1179, FGA
- PowerPlex ESI 17:
Amelogenin, D3S1358,
D19S433, D2S1338,
D22S1045,
D16S539,
D18S51, D1S1656,
D10S1248, D2S441,
TH01, VWA, D21S11,
D12S391,
D8S1179, FGA, SE33
- PowerPlex 21: Amelogenin, D3S1358, D1S1656,
D6S1043,
D13S317,
Penta E,
D16S539,
D18S51, D2S1338, CSF1PO,
Penta D,
TH01, VWA, D21S11, D7S820, D5S818, TPOX,
D8S1179,
D12S391,
D19S433, FGA
- Silver Stain Systems:
- CTT: CSF1PO, TPOX, TH01
- CTTV : CSF1PO, TPOX, TH01, VWA
- FFV: F13A1, FESFPS, VWA
- FFFL : F13A1, FESFPS, F13B, LPL
- GammaSTRTM: D16S539, D7S820, D13S317, D5S818
STR Kits from
QIAGEN N.V.
(Venlo, NL)
STR Kits from
Biotype (Dresden, Germany)
-
Mentype®
Nonaplex I: Amelogenin,
D3S1358, TH01, SE33, vWA, FGA, D18S51, D8S1179, and D21S11
-
Mentype®
NonaplexQS:
Amelogenin, D8S1179, D21S11, D18S51, TH01, D3S1358, SE33, vWA,
and FGA
-
Mentype®
NonaplexQS Twin:
combined Nonaplex I and NonaplexQS kits
-
Mentype®
Pentaplex ESS:
Amelogenin, D22S1045, D12S391, D10S1248, D1S1656, D2S441
-
Mentype®
Argus Y-MHQS:
DYS19, DYS385 a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392,
DYS393
-
Mentype®
Argus X-8: DXS8378, HPRTB,
DXS7423, DXS7132, DXS10134, DXS10074, DXS10101, and DXS10135
as well as
Amelogenin
- Mentype®
Chimera®: Amelogenin, D7S1517,
D3S1744, D12S391, D2S1360, D6S474, D8S1132, D5S2500, D18S51, D21S2055,
D10S2325, SE33, D4S2366
- Genome LabTM Human STR Primer Set:
TH01, D18S51,
amelogenin, D13S317,
D16S539, Penta E,
D3S1358, D8S1179,
TPOX, CSF1PO,
Penta D
Explanation
of STR Multiplexes:
The desire to gain more information from a sample, coupled
with the need to limit consumption of a DNA sample where its
availability may be limited (such as evidence from a crime scene)
has led to the co-amplification and typing of multiple STR
systems. Multiplex PCR, which involves adding more
than one set of PCR primers to the reaction in order to target
multiple locations throughout the genome, is an ideal technique
for DNA typing because the probability of identical alleles in
two individuals decreases with an increase in the number of
polymorphic loci examined. The advent of fluorescent labeling
of the PCR primers permits the multiplexing of
STR loci which may have alleles that fall in the same size range by labeling the
overlapping loci with different colored fluorescent dyes that can then be
resolved spectrally.
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Last Updated:
12/03/2012