Project Number: 1245-21000-263-33
Project Type: Specific Cooperative Agreement

Start Date: Feb 23, 2010
End Date: Sep 30, 2014

Objective:
The long-term objective of this work is the discovery of genes or other genetic factors that control soybean yield potential. The specific objective of this research is the creation of large set of recombinant inbred lines (RILs) derived from the cross of the elite soybean genotype IA3023 with a diverse set of cultivars and germplasm accessions and the analysis of the RILs with 1536 single nucleotide polymorphism (SNP) DNA markers.

Approach:
A total of 1200 F2 plants from each of 40-50 matings between IA3023 and high yielding elite and exotic soybean lines will be planted in the field. At least 1000 F2 plants from each of those matings with maturity as similar as possible to the common Maturity Group III parent IA3023 will be harvested via single-seed descent (SSD) and the 1000 F3 seed of each mating will be shipped to a winter nursery to initiate a two-generation SSD advance to produce first F4 then F5 seed. The F5 plants will be grown in the field and leaf tissue will be harvested from each F5 plant for DNA extraction. Seed will be harvested from a minimum of 500 F5 plants per mating and increased in a winter nursery to produce sufficient seed of 250 RILs per mating for yield testing in replicated yield trials. Each of the RILs will also be characterized with 1536 SNP DNA markers using the Illumina GoldenGate assay.