Testing Status of Agents at NTP

1,3-Butadiene

http://ntp.niehs.nih.gov/go/TS-10514-J

CASRN: 106-99-0

Formula: C4-H6

Synonyms/Common Names:

  • ALPHA,GAMMA-BUTADIENE

Known Uses:

Chemical intermediate. Manufacture of synthetic rubber and of thermoplastic resins. Produced from petroleum gases (Merck 1989). Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Short-Term Toxicity

  • 13-WEEK (Inhalation)  (C50602B)  Completed 
    • Rats: F344/N
    • Dose: R: 0, 200, 625, 2000, OR 6250 PPM M: 0, 625, 1250, 5000, 8000, OR 16000 PPM.

Long-Term Carcinogenicity

  • 2-YEAR (Inhalation)  (C50602A)  Report Complete 
    • TR-288 (NIH Number: 84-2544; NTIS Number: PB85-179646)  (Peer Review Approval 10/28/1983A )
      Toxicology and Carcinogenesis Studies of 1,3-Butadiene (CASRN 106-99-0) in B6C3F1 Mice (Inhalation Studies)
    • Mice: B6C3F1
    • Carcinogenesis Results
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: 0,625,1250 PPM/50 PER GROUP.
  • 2-YEAR (Inhalation)  (C50602C)  Report Complete 
    • TR-434 (NIH Number: 92-3165; NTIS Number: PB94-101631)  (Peer Review Approval 11/21/1991A )
      Toxicology and Carcinogenesis Studies of 1,3-Butadiene (CASRN 106-99-0) in B6C3F1 Mice (Inhalation Studies)
    • Mice: B6C3F1
    • Carcinogenesis Results
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: 0, 6.25, 20, 62.5, 200, 625 PPM/50 PER GROUP.
  • 2-YEAR (Inhalation)  (C50602D)  Completed 
    • Citation: Bucher JR, Melnick RL, and Hildebrandt PK. Lack of Carcinogenicity in Mice Exposed Once to High Concentrations of 1,3-Butadiene. J. Nat. Cancer Inst 85: 1866-1867 (1993).
    • Mice: B6C3F1
    • Dose: MICE ONLY: 0, 1000, 5000, 10000 PPM/60 PER GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0220)  Completed 
    • Citation: DAHL, A.R., BECHTOLD, W.E., BOND, J.A., HENDERSON, R.F., MAUDERLY, J.L., MUGGENBURG, B.S., DUN, J.D., AND BIRNBAUM, L.S. SPECIES DIFFERENCES IN THE METABOLISM AND DISPOPSITION OF INHALED 1,3-BUTADIENE AND ISOPRENE. ENVIRONMENTAL HLTH PERSPECTIVES, VOL 86, PP. 65-69, 1990
    • Rats; Mice; Monkey
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0792)  Completed 
    • Citation: BOND, J.A., DAHL, A.R., HENDERSON, R.F., DUTCHER, J.S., MAUDERLLY, J.L., AND BIRNBAUM, L.S. SPECIES DIFFERENCES IN THE DISPOSITION OF INHALED BUTADIENE. TOXICOLOGY AQND APPLIED PHARMACOLOGY 84, 612-627 (1986)
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0793)  Completed 
    • Citation: BOND, J.A., MARTIN, O.S., BIRNBAUM, L.S., DAHL, A.R., MELNICK, R.L., AND HENDERSON, R.F. METABOLISM OF 1,3-BUTADIENE BY LUNG AND LIVER MICROSOMES OF RATS AND MICE R EPEATEDLY EXPOSED BY INHALATION TO 1,3-BUTADIENE. TOXICOLOGY LETTERS, 44 (1988) 143-151.
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0794)  Completed 
    • Citation: BOND, J.A., DAHL, A.R., HENDERSON, R.F., AND IRNBAUM, L.S. SPECIES DIFFERENCES IN THE DISTRIBUTION OF INHALED BUTADIENE IN TISSUES. AM. IND. HYG. ASSOC. J. (48) 867-872, (1987)
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0795)  Completed 
    • Citation: SABOURIN, P.J., BURKA, L.T., BECHTOLD, W.E., DAHL, A.R., HOOVER, M.D., CHANG, I.Y., AND HENDERSON, R.F. SPECIES DIFFERENCES IN URINARY BUTADIENE METABOLITES; IDENTIFICATION OF 1,2-DIHYDROXY-4- (N-ACETYLCYSTEINYL) BUTANE, A NOVEL METABOLITE OF BUTADIENE CARCINOGENESIS, VOL 13, NO. 9, PP. 1633-1638, 1992
    • Rats: F344/N; Rats: Sprague Dawley; Mice: B6C3F1; Hamsters: Syrian Golden; Monkey
  • Other (Intraperitoneal Injection)  (S0459)  Completed 
    • Citation: BIRNBAUM, ET.AL., CHARACTERIZATION OF HEMOGLOBIN ADDUCT FORMATION IN MICE & RATS AFTER ADMINISTRATION OF [14C]BUTADIENE OR [14C]ISOPRENE, TOXICOL & APPL PHARM 100:86-95,1989.
    • Male Rats: Sprague Dawley; Male Mice: B6C3F1
    • Dose: 1,10,100 OR 1000 UMOL [14C]BUTADIENE.
  • Toxicokinetic Study (Inhalation)  (S0796)  Completed 
    • Citation: DAHL, A.R., SUN, J.D., BIRNBAUM, L.S., BOND, J.S., GRIFFITH, W.C. JR, MAUDERLY, J.L., MUGGENBURG, B.S., SABOURIN, P.J., AND HENDERSON, R.F. TOXICOKINETICS OF INHALED 1,3-BUTADIENE IN MONKEYS: COMPARISON TO TOXICOKINETICS OF RATS AND MICE. TOXICOLOGY AND APPLIED PHARMACOLOGY 110, 9-19 (1991)
    • Rats; Mice; Monkey

Genetic Toxicology

  • Chromosome Aberrations  (A74454)  Completed 
    • Citation: Tice, R.R., Boucher, R., Luke, C.A., and Shelby, M.D. Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutagen Vol. 9 (1987) 235-250
    • Mice
  • Chromosome Aberrations  (A93093)  Completed 
    • Mice
    • Chromosome Aberation  Positive 
  • Drosophila  (950073)  Completed 
    • Citation: Foureman, P., Mason, J.M., Valencia, R., Zimmering, S. Chemical Mutagenesis Testing in Drosophila. IX. Results of 50 coded compounds tested for the National Toxicology Program. Environ. Molec. Mutagen. Vol. 23 (1994) 51-63
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Mouse Lymphoma  (614566)  Completed 
    • Citation: McGregor, D., Brown, A.G., Cattanach, P., Edwards, I., McBride, D., Riach, C., Shepherd, W., and Caspary, W. Responses of the L5178Y mouse lymphoma forward mutation assay: V. Gases and vapors. Environ. Molec. Mutagen. Vol. 17 (1991) 122-129
    • Mice
    •  Negative 
  • Micronucleus  (A74454)  Completed 
    • Citation: Tice, R.R., Boucher, R., Luke, C.A., and Shelby, M.D. Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutagen Vol. 9 (1987) 235-250
    • Mice: B6C3F1
    • Male Positive (Nonstandard Protocol) 
  • Salmonella  (256166)  Completed 
    •  Positive 
  • Sister Chromatid Exchanges  (A74454)  Completed 
    • Citation: Tice, R.R., Boucher, R., Luke, C.A., and Shelby, M.D. Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutagen Vol. 9 (1987) 235-250
    • Mice

Organ Systems Toxicity

  • 5 consecutive days Dominant Lethal (Inhalation)  (T0183A)  Completed 
  • 6 to 15 GD Conventional Teratology (Inhalation)  (TER85144)  Completed 
    • NTIS Number: DE88004187
      Citation: Inhalation Developmental Toxicology Studies: Teratology Study of 1,3-Butadiene in Mice (November 1987)
    • Female Mice: Swiss
    • Mice Maternal Positive 
    • Mice Fetal Positive 
    • Dose: 0, 40, 200, 1000 PPM.
  • 6 to 19 GD Conventional Teratology (Inhalation)  (TER89041)  Completed 
    • NTIS Number: DE88004186
      Citation: Inhalation Developmental Toxicology Studies of 1,3-Butadiene in the Rat (November 1987)
    • Female Rats: Sprague Dawley
    • Rats Maternal Negative 
    • Rats Fetal Negative 
    • Dose: 0, 40, 200, 1000 PPM.

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