Materials and Reagents

From Assay Guidance Wiki

The list below includes materials and reagents, which have been used successfully to enable GTPγS binding assays for a variety of G-Protein Coupled Receptors:

• 96 well plates: Costar 3632, white clear bottom
• WGA SPA beads: Amersham SPQ0031
• Anti-rabbit SPA beads: Amersham RPNQ 0016
• Anti-mouse SPA beads: Amersham Care RPNQ 0017
• GTPγ35S: Perkin Elmer Life Sciences NEG030H
• NP40 detergent 10%: Roche 1 332 473
• Anti-Gs/olf: Santa Cruz SC-383, rabbit polyclonal
• Anti-Gi3: Santa Cruz SC-262, rabbit polyclonal (recognizes Gi-1, Gi-2, and Gi-3)
• Anti-Gq/11: Santa Cruz SC-392, rabbit polyclonal
• Anti-Go: Chemicon MAB3073, mouse monoclonal