FBI Headquarters, which is located in Washington, D.C, houses many operational, service, and support divisions that work together to coordinate national and international operations. FBI Headquarters is organized into five main branches, each of which is composed of multiple divisions that carry out the Bureau's mission.

• National Security Branch
• Counterterrorism Division
• Counterintelligence Division
• Directorate of Intelligence
• Weapons of Mass Destruction Directorate
• Criminal, Cyber, Response, and Services Branch
• Criminal Investigative Division
• Cyber Division
• Critical Incident Response Group
• International Operations Division
• Office of Law Enforcement Coordination
• Human Resources Branch
• Training Division
• Human Resources Division
• Science and Technology Branch
• Criminal Justice Information Services Division
• Laboratory Division
• Operational Technology Division
• Special Technologies and Applications Office
• Information Technology Branch
• Office of the Deputy Chief Information Officer
• Information Technology Services Division
• Information Technology Management Division
• Information Technology Engineering Division & The Chief Technology Officer
• Office of the Chief Knowledge Officer

There are also several important Divisions/Offices that report directly to the Director and Deputy Director:

• Office of Congressional Affairs
• Office of Equal Employment Opportunity Affairs
• Office of the General Counsel
• Office of Integrity and Compliance
• Office of the Ombudsman
• Office of Professional Responsibility
• Office of Public Affairs
• Inspection Division
• Facilities & Logistics Services Division
• Finance Division
• Records Management Division
• Resource Planning Office
• Security Division