Molecular Detection of Drug Resistance (MDDR)
Intended use | Rapid detection of drug resistance in Mycobacterium tuberculosis isolates | ||||||||||||||||||||||||||||||||||||
Assay | DNA sequencing will be performed using the ABI 3130xl. Sequencing will be performed from PCR products amplified from genetic loci associated with resistance to first- and second-line anti-tuberculosis drug. DNA sequencing was chosen as the platform due to its automation, high-throughput and potential for expansion (additional loci can be added to the panel). | ||||||||||||||||||||||||||||||||||||
Loci to be sequenced and associated drug resistance | First-line MDDR to detect MDR-TB
Second-line MDDR to detect XDR-TB
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Performance |
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Limitations |
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Submission criteria | Must meet one of the following criteria:
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Sample types accepted | Isolates only (one per patient)
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Submission guidelines |
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Conventional DST | All isolates submitted for MDDR will also undergo conventional DST by the agar proportion method (PZA by MGIT 960) | ||||||||||||||||||||||||||||||||||||
Reporting |
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References |
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MDDR Contact Information:
Centers for Disease Control and Prevention
Division of Tuberculosis Elimination
Laboratory Branch
1600 Clifton Road, NE, MS F08
Atlanta, GA 30333
Telephone: 404-639-2455
FAX: 404-639-5491
Email: TBLab@cdc.gov
Contact Us:
- Centers for Disease Control and Prevention
Division of Tuberculosis Elimination (DTBE)
1600 Clifton Rd., NE
MS E10
Atlanta, GA 30333 - 800-CDC-INFO
(800-232-4636)
TTY: (888) 232-6348 - New Hours of Operation
8am-8pm ET/Monday-Friday
Closed Holidays - cdcinfo@cdc.gov