National Human Genome Research Institute   National Institutes of Health
U.S. Department of Health and Human Services

Robert W. Blakesley, Ph.D.

Associate Investigator, Genome Technology Branch
Director, Sequence Finishing Group, NIH Intramural Sequencing Center [nisc.nih.gov]

Selected Publications

Fuchs R., Blakesley R. Guide to the use of type II restriction endonucleases. Methods Enzymol, 100:3-38. 1983. [PubMed]

Thompson J.A., Blakesley R.W., Doran K., Hough C.J., Wells R.D. Purification of nucleic acids by RPC-5 ANALOG chromatography: peristaltic and gravity-flow applications. Methods Enzymol, 100:368-399. 1983. [PubMed]

Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F. Genetic organization of the KpnI restriction-modification system. Nucleic Acids Res, 19(23):6505-6509. 1991. [PubMed]

Wang G., Blakesley R.W., Berg D.E., Berg C.M. pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo. Proc Natl Acad Sci U S A, 90(16): 7874-7878. 1993. [PubMed]

Blakesley R.W. Cycle sequencing. Methods Mol Biol, 167:101-112. 2001. [PubMed]

DeSilva U., Elnitski L., Idol J.R., Doyle J.L., Gan W., Thomas J.W., Schwartz S., Dietrich N.L., Beckstrom-Sternberg S.M., McDowell J.C., Blakesley R.W., Bouffard G.G., Thomas P.J., Touchman J.W., Miller W., Green E.D. Generation and comparative analysis of ~3.3 mb of mouse genomic sequence orthologous to the region of human chromosome 7q11.23 implicated in Williams syndrome. Genome Res, 12(1):3-15. 2002. [PubMed]

Shevchenko Y., Bouffard G.G., Butterfield Y.S., Blakesley R.W., Hartley J.L., Young A.C., Marra M.A., Jones S.J., Touchman J.W., Green E.D. Systematic sequencing of cDNA clones using the transposon Tn5. Nucleic Acids Res, 30(11):2469-77. 2002. [PubMed]

Thomas, J.W., Schueler, M.G., Summers, T.J., Blakesley, R.W., McDowell, J.C., Thomas, P.J., Idol, J.R., Maduro, V.V.B., Lee-Lin, S.-Q., Touchman, J.W., Bouffard, G.G., Beckstrom-Sternberg, S.M., NISC Comparative Sequencing Program, and Green, E.D. Pericentromeric Duplication in the Laboratory Mouse. Genome Res, (13):55-63. 2003. [PubMed]

Thomas J.W., Touchman J.W., Blakesley R.W., Bouffard G.G., Beckstrom-Sternberg S.M., Margulies E.H., Blanchette M., Siepel A.C., Thomas P.J., McDowell J.C., Maskeri B., Hansen N.F., Schwartz M.S., Weber R.J., Kent W.J., Karolchik D., Bruen T.C., Bevan R., Cutler D.J., Schwartz S., Elnitski L., Idol J.R., Prasad A.B., Lee-Lin S-Q., Maduro V.V.B., Summers T.J., Portnoy M.E., Dietrich N.L., Akhter N., Ayele K., Benjamin B., Cariaga K., Brinkley C.P., Brooks S.Y., Granite S., Guan X., Gupta J., Haghighi P., Ho S-L., Huang M.C., Karlins E., Laric P.L., Legaspi R., Lim M.J., Maduro Q.L., Masiello C.A., Mastrian S.D., McCloskey J.C., Pearson R., Stantripop S., Tiongson E.E., Tran J.T., Tsurgeon C., Vogt J.L., Walker M.A., Wetherby K.D., Wiggins L.S., Young A.C., Zhang L-H., Osoegawa K., Zhu B., Zhao B., Shu C.L., De Jong P.J., Lawrence C.E., Smit A.F., Chakravarti A., Haussler C., Green P., Miller W., and Green E.D. Comparative analyses of multi-species sequences from targeted genomic regions. Nature, 424(6950):788-793. 2003. [PubMed]

Crawford G.E., Holt I.E., Mullikin J.C., Tai D., Blakesley R., Bouffard G., Young A, Masiello C., Green E.D., Wolfsberg T.G, Collins F.S.; National Institutes Of Health Intramural Sequencing Center. Identifying gene regulatory elements by genome-wide recovery of DNase hypersensitive sites. Proc Natl Acad Sci U S A, 101(4):992-7. 2004. [PubMed]

Blakesley, R.W., Hansen, N.F., Mullikin, J.C., Thomas, P.J., McDowell, J.C., Maskeri, B., Young A.C., Benjaminn, B., Brooks, S.Y., Coleman, B.I., Gupta, J., Ho, S-L., Karlins, E.M., Maduro, Q.L., Stantripop, S., Tsurgeon, C., Vogt, J.L., Walker, M.A., Masiello, C.A., Gua, X., NISC Comparative Sequencing Program, Bouffard, G.G., and Green, E.D. An intermediate grade of finished genomic sequence suitable for comparative analysis. Genome Research. 2004. [PubMed]

Margulies E.H., Cooper, G.M., Asimenos, G., Thomas, D.J., Dewey, C.N., Siepel, A., Birney, E., Keefe, D., Schwartz, A.S., Hou, M., Taylor, J., Nikolaev, S., Montoya-Burgos, J.I., Löytynoja, A., Whelan, S., Pardi, F., Massingham, T., Brown, J.B., Bickel, P., Holmes, I., Mullikin, J.C., Ureta-Vidal, A., Paten, B., Stone, E.A., Rosenbloom, K.R., Kent, W.J., Bouffard, G.G., Guan, X., Hansen, N.F., Idol, J.R., Maduro, V.V., Maskeri, B., McDowell, J.C., Park, M., Thomas, P.J., Young, A.C., Blakesley, R.W., Muzny, D.M., Sodergren, E., Wheeler, D.A., Worley, K.C., Jiang, H., Weinstock, G.M., Gibbs, R.A., Graves, T., Fulton, R., Mardis, E.R., Wilson, R.K., Clamp, M., Cuff, J., Gnerre, S., Jaffe, D.B., Chang, J.L., Lindblad-Toh, K., Lander, E.S., Hinrichs, A., Trumbower, H., Clawson, H., Zweig, A., Kuhn, R.M., Barber, G., Harte, R., Karolchik, D., Field, M.A., Moore, R.A., Matthewson, C.A., Schein, J.E., Marra, M.A., Antonarakis, S.E., Batzoglou, S., Goldman, N., Hardison, R., Haussler D., Miller, W., Pachter, L., Green, E.D., Sidow, A. Analyses of deep mammalian sequence alignments and constraint predictions for 1% of the human genome. Genome Res, 17(6):760-74. 2007. [PubMed]

The ENCODE Project Consortium. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature, 447: 799-816. 2007. [PubMed]

Top of page

Last Reviewed: January 6, 2009