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Jörg Schwender
Brookhaven National Laboratory
Bldg. 463 - P.O. Box 5000
Upton, NY 11973-5000
Phone: |
(631) 344-3797 |
Lab Phone: |
(631) 344-4262 |
Fax: |
(631) 344-3407 |
email: |
schwend@bnl.gov |
Recent News
Modeling Plant Metabolism To Optimize Oil Production
Background
Plant biomass is of increasing importance as a renewable resource for the production of fuels and of chemical
feedstocks that replace petroleum based materials. As a basis for rational engineering of seeds and other plant
storage organs, our goal is to increase the basic understanding of the functioning of storage metabolism in plants.
Of central interest here is the process of allocation of maternal carbon and nitrogen resources to different
storage products (oil, protein and starch) in a sink organ like a developing seed.
![](https://webarchive.library.unt.edu/web/20121007144005im_/http://www.bnl.gov/biology/Plantbio/images/BigFluxMap-sm.jpg)
Schematic of carbon flow during storage synthesis in developing seeds of Brassica napus based on metabolic
flux analysis (See publications below). PPP = pentose phosphate pathway; TAG = triacylglycerol.
Research Interests
Within the interest in carbon partitioning in sink organs in plants, our research is focused on metabolic flux
analysis and pathway analysis by employing isotope labeling experiments, mathematical models and computer simulation
to describe and analyze metabolism quantitatively. In particular we use steady-state stable isotope labeling to
determine the flux distribution in central carbon metabolism. Cultures of developing embryos of Brassica napus
and other brassicaceae turned out to be excellent in vitro models to study seed development and storage synthesis.
Growing in culture, embryos are labeled with a variety of 13C-labeled precursors and individual C-atoms are traced
through the metabolic network by analyzing the label in metabolites and end products by GC/MS and NMR. Different
nutritional conditions or genotypes can be analyzed and compared.
In order to quantitatively characterize and measure metabolism as comprehensively as possible, we consider enzyme
activity and metabolite levels as important metrics in addition to metabolic flux. In collaboration with Alistair
Rogers (BNL, Environmental Sciences Department) and Hardy Rolletschek (Institute for Plant Genetics and Crop Plant
Research, Gatersleben) enzyme and metabolite profiles are measured in parallel with flux. The principles of Metabolic
Control Analysis and enzyme kinetic models help to interpret these data and to model and understand the allosteric
regulation in central metabolism.
Published Books
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Plant Metabolic Networks
Schwender, Jörg (Ed.)
2009, X, 390 p. 13 illus., 11 in color., Springer, NY, Hardcover ISBN: 978-0-387-78744-2
Selected Publications
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O’Gready J., Schwender J., Shachar-Hill Y., and Morgan J.A.
Metabolic cartography: experimental quantification of metabolic fluxes from isotopic labelling studies.
J. Exp. Botany, doi: 10.1093/jxb/ers032 (2012).
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Hay J. and Schwender J.
Metabolic network reconstruction and flux variability analysis of storage synthesis in developing oilseed rape (Brassica napus L.) embryos.
The Plant Journal 67, 526–541 (2011).
PubMed
Abstract
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Hay J. and Schwender J.
Computational analysis of storage synthesis in developing Brassica napus L. (oilseed rape) embryos: flux variability analysis in relation to ¹³C metabolic flux analysis.
The Plant Journal 67, 513–525 (2011).
PubMed
Abstract
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Lohr M., Schwender J., and Polle J.E.W.
Isoprenoid biosynthesis in eukaryotic phototrophs: A spotlight on algae.
Plant Sciences. doi:10.1016/j.plantsci.2011.07.018 (2011).
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Schwender J.
Experimental flux measurements on a network scale.
Frontiers in Plant Sci. 2:63. doi: 10.3389/fpls.2011.00063 (2011).
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Koschützki D., Junker B.H., Schwender J., and Schreiber F.
Structural analysis of metabolic networks based on flux centrality.
J. Theor Biol., 265:261-269, DOI 10.1016/j.jtbi.2010.05.009 (2010).
PubMed
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Lonien J., and Schwender J.
Analysis of metabolic flux phenotypes for two Arabidopsis thaliana mutants with severe impairment in seed storage lipid synthesis.
Plant Physiology, 151(3):1617-1634 (2009).
PubMed
Plant Physiology
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van der Lelie D., Taghavi S., Monchy S., Schwender J., Miller L., Ferrieri R., Rogers A., Wu X., Zhu W., Weyens N., Vangronsveld J., and Newman L.
Poplar and its bacterial endophytes: Coexistence and harmony.
Critical Reviews in Plant Sciences 28(5), 346-358 (2009).
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Schwender J.
Metabolic flux analysis as a tool in metabolic engineering of plants.
Curr. Opin. Biotechnol., 19(2):131-137 (2008).
PubMed
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Junker B.H., Lonien J., Heady L.E., Rogers A. and Schwender J.
Parallel determination of enzyme activities and in vivo fluxes in Brassica napus embryos grown on organic or inorganic
nitrogen source.
Phytochemistry, 68(16-18):2232-2242 (2007).
PubMed
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Schwender J., Shachar-Hill Y. and Ohlrogge, J.B.
Mitochondrial metabolism in developing embryos of Brassica napus.
J Biol Chem., 281:34040-34047 (2006).
PubMed
See also:
J Biol Chem of November 10, (2006).
Paper of the Week
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Goffman F.D., Alonso A.P., Schwender J., Shachar-Hill Y. and Ohlrogge, J.B.
Light enables a very high efficiency of carbon storage in developing embryos of rapeseed.
Plant Physiol., 138(4):2269-2279 (2005).
PubMed
Full Text
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Ruuska S.A., Schwender J. and Ohlrogge J.B.
The capacity of green oilseeds to utilize photosynthesis to drive biosynthetic processes.
Plant Physiol., 136(1):2700-2709 (2004).
PubMed
Full Text
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Schwender J., Goffman F., Ohlrogge J.B. and Shachar-Hill Y.
Rubisco without the Calvin cycle improves the carbon efficiency of developing green seeds.
Nature, 432:779-782 (2004).
PubMed
See also:
CH Surridge, Nature News and Views:
Plant biochemistry: Green catalytic converter.
Nature, 432:684 (2004).
PubMed
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Schwender J., Ohlrogge J.B. and Shachar-Hill Y.
Understanding flux in plant metabolic networks.
Curr Opin Plant Biol., 7(3):309-317 (2004).
PubMed
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Schwender J., Ohlrogge J.B. and Shachar-Hill Y.
A flux model of glycolysis and oxidative pentosephosphate pathway in developing
Brassica napus embryos.
J Biol Chem., 278(32):29442-29453 (2003).
PubMed
Full Text
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Schwender J. and Ohlrogge J.B.
Probing in vivo metabolism by stable isotope labeling of storage lipids and proteins in developing Brassica napus embryos.
Plant Physiol., 130(1):347-361 (2002).
PubMed
Full Text
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