NIST Authors in Bold
Author(s): | Gallatin, G. M.; Berglund, A. J.; |
---|---|
Title: | Optimal Laser Scan Path for Localizing a Fluorescent Particle in Two or Three Dimensions |
Published: | July 16, 2012 |
Abstract: | Localizing a fluorescent particle by scanning a focused laser in its vicinity and analyzing the detected photon stream provides real-time information for a modern class of feedback control systems for particle tracking and trapping. We present a simple mathematical test to determine whether a particular laser scan path encodes maximal information about a fluorescent particle's position in the detected photon stream. We give the optimal path for a Gaussian laser scanned in two or three dimensions, and present the Cram\'{e}r-Rao bound on the two and three-dimensional localization accuracy that can be achieved by analyzing the detected photon stream. |
Citation: | Optics Express |
Volume: | 20 |
Issue: | 15 |
Pages: | pp. 16381 - 16393 |
Keywords: | single particle tracking |
Research Areas: | Optics, Optical microscopy |
PDF version: | Click here to retrieve PDF version of paper (862 K) |