Projects

IDENTIFICATION OF HOST IMMUNE FACTORS AND INTERVENTION STRATEGIES FOR MASTITIS Appropriated (D)   Accession Number: 421859

TRANSCRIPTOMICS OF THE IMMUNE CELL RESPONSE TO A BACTERIAL INFECTION IN THE MAMMARY GLAND Trust (T)   Accession Number: 421614

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Vitamin D signaling in the bovine immune system: A model for understanding human vitamin D requirements - (Peer Reviewed Journal)

Nelson, C.D., Reinhardt, T.A., Lippolis, J.D., Sacco, R.E., Nonnecke, B.J. 2012. Vitamin D signaling in the bovine immune system: A model for understanding human vitamin D requirements. Nutrients. 4(3):181-196.

Differential expression of cytokines in response to respiratory syncytial virus infection of calves with high or low circulating 25-hydroxyvitamin D3 - (Peer Reviewed Journal)

Sacco, R.E., Nonnecke, B.J., Palmer, M.V., Waters, W.R., Lippolis, J.D., Reinhardt, T.A. 2012. Differential expression of cytokines in response to respiratory syncytial virus infection of calves with high or low circulating 25-hydroxyvitamin D3. PLoS One. 7(3):e33074. Available: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0033074.

Bovine milk exosome proteome - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D., Nonnecke, B.J., Sacco, R.E. 2012. Bovine milk exosome proteome. Journal of Proteomics. 75(5):1486-1492.

Treatment of an intramammary bacterial infection with 25-hydroxyvitamin D3 - (Peer Reviewed Journal)

Lippolis, J.D., Reinhardt, T.A., Sacco, R.E., Nonnecke, B.J., Nelson, C.D. 2011. Treatment of an intramammary bacterial infection with 25-hydroxyvitamin D3. PLoS One. 6(10):e25479. Available: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184989/pdf/pone.0025479.pdf.

Innate immune response to a bovine mastitis pathogen profiled in milk and blood monocytes using a systems biology approach - (Abstract Only) - (19-Aug-11)

Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3 - (Peer Reviewed Journal)

Nelson, C.D., Nonnecke, B.J., Reinhardt, T.A., Waters, W.R., Beitz, D.C., Lippolis, J.D. 2011. Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3. PLoS One. 6(6):e21674. Available: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021674.

Utility, Limitations, and Promise of Proteomics in Animal Science - (Peer Reviewed Journal)

Lippolis, J.D., Reinhardt, T.A. 2010. Utility, limitations, and promise of proteomics in animal science. Veterinary Immunology and Immunopathology. 138(4):241-251.

In vivo activation of the intracrine vitamin D pathway in innate immune cells and mammary tissue during a bacterial infection - (Peer Reviewed Journal)

Nelson, C.D., Reinhardt, T.A., Beitz, D.C., Lippolis, J.D. 2010. In vivo activation of the intracrine vitamin D pathway in innate immune cells and mammary tissue during a bacterial infection. PLoS One. 5(11):e15469. Available: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015469.

Methods and Approaches to Mass Spectroscopy Based Protein Identification - (Book / Chapter)

Lippolis, J.D., Reinhardt, T.A. 2011. Methods and approaches to mass spectroscopy based protein identification. In: Whitfield, P.D. Eckersall, D., editors. Methods in animal proteomics. West Sussex, UK: Wiley-Blackwell. p. 77-102.

Prevalence of Subclinical Hypocalcemia in Dairy Herds - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D., Mccluskey, B.J., Goff, J.P., Horst, R.L. 2011. Prevalence of Subclinical Hypocalcemia in Dairy Herds. The Veterinary Journal. 188:122-124.

1,25-Dihydroxyvitamin D3 Enhances Innate Immune Responses of Bovine Mammary Epithelial Cells that are Triggered by Toll-like Receptor Signaling - (Abstract Only)

Nelson, C.D., Reinhardt, T.A., Beitz, D.C., Lippolis, J.D. 2010. 1,25-Dihydroxyvitamin D3 Enhances Innate Immune Responses of Bovine Mammary Epithelial Cells that are Triggered by Toll-like Receptor Signaling [abstract]. Midwest Branch of the American Dairy Science Association. p. 30.

Vitamin D Signaling in the Bovine Mammary Gland is Part of the Innate Immune Response to Bacterial Pathogens - (Abstract Only)

Nelson, C.D., Reinhardt, T.A., Lippolis, J.D. 2010. Vitamin D Signaling in the Bovine Mammary Gland is Part of the Innate Immune Response to Bacterial Pathogens [abstract]. National Mastitis Council. p. 196.

Modulation of the Bovine Innate Immune Response by Production of 1alpha,25-Dihydroxyvitamin D3 in Bovine Monocytes - (Peer Reviewed Journal)

Nelson, C.D., Reinhardt, T.A., Thacker, T.C., Beitz, D.C., Lippolis, J.D. 2010. Modulation of the Bovine Innate Immune Response by Production of 1alpha,25-Dihydroxyvitamin D3 in Bovine Monocytes. Journal of Dairy Science. 93(3):1041-1049.

The Utility, Limitations, and Promise of Proteomics in Animal Science - (Abstract Only) - (30-Sep-09)

Improvement of the Innate Immune Response of Bovine Monocytes Via an Intracrine Vitamin D Signaling Pathway - (Abstract Only)

Nelson, C.D., Reinhardt, T.A., Beitz, D.C., Lippolis, J.D. 2009. Improvement of the Innate Immune Response of Bovine Monocytes Via an Intracrine Vitamin D Signaling Pathway [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 94.

Expression of Inducible Nitric Oxide Synthase is Up-Regulated by Production of 1,25-Dihydroxyvitamin D3 in Bovine Monocytes in Response to Toll-Like Receptor Signaling - (Abstract Only)

Nelson, C.D., Beitz, D.C., Reinhardt, T.A., Lippolis, J.D. 2009. Expression of Inducible Nitric Oxide Synthase is Up-Regulated by Production of 1,25-Dihydroxyvitamin D3 in Bovine Monocytes in Response to Toll-Like Receptor Signaling [abstract]. American Dairy Science Association. Paper No. 84.

Understanding the Relationship Between Immunity and Dairy Cow Production - (Trade Journal)

Lippolis, J.D. 2009. Understanding the Relationship Between Immunity and Dairy Cow Production. Hoard's Dairyman. 154 (13):481.

Proteomics of mastitis causing Escherichia coli - (Proceedings)

Lippolis, J.D., Reinhardt, T.A., Kehrli Jr, M.E. 2009. Proteomics of mastitis causing Escherichia coli. Proceedings of National Mastitis Council. 48:236-237.

Mammary gland involution is associated with rapid down regulation of major mammary Ca**2+-ATPases - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D. 2009. Mammary gland involution is associated with rapid down regulation of major mammary Ca**2+-ATPases. Biochemical and Biophysical Research Communications. 378(1):99-102.

Proteomic changes in Escherichia coli when grown in fresh milk versus laboratory media - (Peer Reviewed Journal)

Lippolis, J.D., Bayles, D.O., Reinhardt, T.A. 2009. Proteomic changes in Escherichia coli when grown in fresh milk versus laboratory media. Journal of Proteome Research. 8(1):149-158.

The promise of proteomics in animal science - (Abstract Only)

Lippolis, J.D., Reinhardt, T.A. 2008. The promise of proteomics in animal science [abstract]. 2008 Joint Annual Meeting of the American Dairy Science Association/American Society of Animal Science, July 7-11, 2008, Indianapolis, Indiana. 2008 CD-ROM.

Centennial Paper: Proteomics in animal science - (Peer Reviewed Journal)

Lippolis, J.D., Reinhardt, T.A. 2008. Centennial Paper: Proteomics in animal science. Journal of Animal Science. 86(9):2430-2441.

Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D. 2008. Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk. Journal of Dairy Science. 91(6):2307-2318.

Toll-like receptor signaling increases production of 1,25-dihydroxyvitamin D3 in bovine macrophages - (Abstract Only)

Nelson, C.D., Beitz, D.C., Reinhardt, T.A., Lippolis, J.D. 2008. Toll-like receptor signaling increases production of 1,25-dihydroxyvitamin D3 in bovine macrophages [abstract). Experimental Biology 2008. p. 188.

Immunological signaling networks: Integrating the body's immune response - (Peer Reviewed Journal)

Lippolis, J.D. 2008. Immunological signaling networks: integrating the body's immune response. Journal of Animal Science. 86(14 Suppl):E53-E63.

Autocrine signaling mechanism of vitamin D in the bovine innate immune response - (Abstract Only)

Nelson, C.D., Beitz, D.C., Reinhardt, T.A., Lippolis, J.D. 2008. Autocrine signaling mechanism of vitamin D in the bovine innate immune response [abstract]. 2008 American Dairy Science Association, American Society of Animal Science, Midwestern Section, Annual Meeting. p. 37.

Adaptive changes in protein expression in Escherichia coli as a consequence of growth in milk - (Abstract Only)

Lippolis, J.D., Reinhardt, T.A. 2007. Adaptive changes in protein expression in Escherichia coli as a consequence of growth in milk [abstract]. Conference of Research Workers in Animal Diseases. p. 123.

Cumulative physiological events modulate the inflammatory response of the bovine udder to Escherichia coli infections around parturition - (Proceedings)

Burvenich, C., Bannerman, D.D., Lippolis, J.D., Peelman, L., Nonnecke, B.J., Kehrli Jr, M.E., Paape, M.J. 2007. Cumulative physiological events modulate the inflammatory response of the bovine udder to Escherichia coli infections around parturition. Proceedings of the Heifer Mastitis Conference. pp. 75-76. June 24-26, 2007. Ghent, Belgium. . Meeting Abstract.

Developmental changes in milk fat globule membrane proteome expression during the transition from colostrum to milk - (Abstract Only)

Reinhardt, T.A., Lippolis, J.D. 2007. Developmental changes in milk fat globule membrane proteome expression during the transition from colostrum to milk [abstract]. 2007 Joint Meeting-American Dairy Science Association, Poultry Science Association, Asociacion Mexicana de Produccion Animal, American Society of Animal Sciences. p. 58.

Changes in protein expression in Escherichia coli as a consequence of growth in milk whey - (Abstract Only)

Lippolis, J.D., Reinhardt, T.A. 2007. Changes in protein expression in Escherichia coli as a consequence of growth in milk whey [abstract]. 2007 Joint Meeting-American Dairy Science Association, Poultry Science Association, Asociacion Mexicana de Produccion Animal, American Society of Animal Sciences. p. 8.

Cumulative physiological events influence the inflammatory response of the bovine udder to Escherichia coli infections during the transition period - (Review Article)

Burvenich, C., Bannerman, D.D., Lippolis, J.D., Peelman, L., Nonnecke, B.J., Kehrli, Jr., M.E., Paape, M.J. 2007. Cumulative physiological events influence the inflammatory response of the bovine udder to Escherichia coli infections during the transition period. Journal of Dairy Science. 90(E.Suppl.):E39-E54.

Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. - (Peer Reviewed Journal)

Radosevich, T.J., Reinhardt, T.A., Lippolis, J.D., Bannantine, J.P., Stabel, J.R. 2007. Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. Journal of Bacteriology. 189(3):1109-1117.

IDENTIFICATION OF DIFFERENCES IN THE PROTEIN CONTENT OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS PURIFIED PROTEIN DERIVATIVE PREPARATIONS WITH DIFFERENT SKIN TEST SPECIFICITIES - (Abstract Only)

Paustian, M., Robbe Austerman, S., Lippolis, J.D., Stabel, J.R., Bannantine, J.P. 2006. Identification of Differences in the Protein Content of Mycobacterium avium subspecies paratuberculosis Purified Protein Derivative Preparations with Different Skin Test Specificities [abstract]. American Society for Microbiology. Paper No. U-016.

PROTEOME AND DIFFERENTIAL EXPRESSION ANALYSIS OF MEMBRANE AND CYTOSOLIC PROTEIN FROM MYCOBACTERIUM AVIUM SUBS PARATUBERCULOSIS STRAINS K10 AND 187 - (Abstract Only)

Radosevich, T.J., Stabel, J.R., Bannantine, J.P., Lippolis, J.D., Reinhardt, T.A. 2006. Proteome and Differential Expression Analysis of Membrane and Cytosolic Protein from Mycobacterium avium subs paratuberculosis Strains k10 and 187 [abstract]. American Society for Microbiology. p. 112.

NEUTROPHIL EXTRACELLULAR TRAP FORMATION BY BOVINE NEUTROPHILS IS NOT INHIBITED BY MILK - (Peer Reviewed Journal)

Lippolis, J.D., Reinhardt, T.A., Goff, J.P., Horst, R.L. 2006. Neutrophil extracellular trap formation by bovine neutrophils is not inhibited by milk. Veterinary Immunology and Immunopathology. 113(1-2):248-255.

BOVINE MILK FAT GLOBULE MEMBRANE PROTEOME - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D. 2006. Bovine milk fat globule membrane proteome. Journal of Dairy Research. 73(4):406-416.

BOVINE MILK FAT GLOBULE MEMBRANE PROTEOME - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D. 2006. Bovine milk fat globule membrane proteome. Journal of Dairy Research. 73(4):406-416.

CUMULATIVE PHYSIOLOGICAL EVENTS INFLUENCE THE INFLAMMATORY RESPONSE OF THE BOVINE UDDER TO E-COLI INFECTIONS DURING THE TRANSITION PERIOD - (Abstract Only)

Burvenich, C., Kehrli Jr, M.E., Paape, M.J., Bannerman, D.D., Lippolis, J.D. 2006. Cumulative physiological events influence the inflammatory response of the bovine udder to e-coli infections during the transition period. Journal of Dairy Science. 84(Suppl. 1):273.

NEUTROPHIL EXTRACELLULAR TRAP FORMATION: AN IMPORTANT NEUTROPHIL KILLING MECHANISM THAT IS NOT INHIBITED IN MILK - (Abstract Only)

Lippolis, J.D., Reinhardt, T.A., Goff, J.P., Horst, R.L. 2006. Neutrophil extracellular trap formation: an important neutrophil killing mechanism that is not inhibited in milk [abstract]. American Dairy Science Association-American Society of Animal Science 2006 Joint Annual Meeting. p. 94.

DIFFERENTIAL EXPRESSION ANALYSIS OF PROTEINS FROM NEUTROPHILS IN THE PERIPARTURIENT PERIOD AND NEUTROPHILS FROM DEXAMETHASONE-TREATED DAIRY COWS - (Peer Reviewed Journal)

Lippolis, J.D., Peterson-Burch, B.D., Reinhardt, T.A. 2006. Differential expression analysis of proteins from neutrophils in the periparturient period and neutrophils from dexamethasone-treated dairy cows. Veterinary Immunology and Immunopathology. 111(3-4):149-164.

DIFFERENTIAL EXPRESSION ANALYSIS OF IMMUNOSUPPRESSED VERSUS NORMAL NEUTROPHIL PROTEOME - (Abstract Only)

Lippolis, J.D., Reinhardt, T.A. 2005. Differential expression analysis of immunosuppressed versus normal neutrophil proteome [abstract]. American Society for Mass Spectrometry. p. 73.

THE SYSTEMIC AND LOCALIZED INNATE IMMUNE RESPONSE TO INTRAMAMMARY INFECTION WITH STREPTOCOCCUS UBERIS - (Proceedings)

Bannerman, D.D., Paape, M.J., Goff, J.P., Kimura, K., Lippolis, J.D., Hope, J.C. 2005. The systemic and localized innate immune response to intramammary infection with streptococcus uberis [abstract]. National Mastitis Council (NMC) 44th Annual Meeting Proceedings. pp. 211-212.

ENERGY AND PROTEIN EFFECTS ON THE IMMUNE SYSTEM - (Book / Chapter)

Kehrli, Jr., M.E., Neill, J.D., Burvenich, C., Goff, J.P., Lippolis, J.D., Reinhardt, T.A., Nonnecke, B.J. 2006. Energy and protein effects on the immune system. In: Sejrsen, K., Hvelplund, T., Nielsen, M.O., editors. Ruminant Physiology. Wageningen, The Netherlands: Wageningen Academic Publishers. p. 455-471.

ENERGY AND PROTEIN EFFECTS ON THE IMMUNE SYSTEM - (Proceedings) - (30-Aug-04)

PROTEOMIC SURVEY OF BOVINE NEUTROPHILS - (Peer Reviewed Journal)

Lippolis, J.D., Reinhardt, T.A. 2005. Proteomic survey of bovine neutrophils. Veterinary Immunology and Immunopathology. 103(1-2):53-65.

NULL MUTATION IN THE GENE ENCODING PLASMA MEMBRANE CA2+-ATPASE ISOFORM 2 IMPAIRS CALCIUM TRANSPORT INTO MILK - (Peer Reviewed Journal)

Reinhardt, T.A., Lippolis, J.D., Shull, G.E., Horst, R.L. 2004. Null mutation in the gene encoding plasma membrane CA2+-ATPase isoform 2 impairs calcium transport into milk. Journal of Biological Chemistry. 279(41):42369-42373.

INNATE IMMUNE RESPONSE TO INTRAMAMMARY INFECTION WITH SERRATIA MARCESSENS AND STREPTOCOCCUS UBERIS - (Peer Reviewed Journal)

Bannerman, D.D., Paape, M.J., Goff, J.P., Kimura, K., Lippolis, J.D., Hope, J.C. 2004. Innate immune response to intramammary infection with serratia marcessens and streptococcus uberis. Veterinary Research. 35(6):681-700.

COMPARATIVE PROTEOMIC ANALYSIS OF NORMAL AND PERIPARTURIENT BOVINE NEUTROPHILS DEMONSTRATING A REDUCTION OF MYELOPEROXIDASE DURING TRANSITION - (Abstract Only)

Lippolis, J.D., Goff, J.P., Reinhardt, T.A. 2004. Comparative proteomic analysis of normal and periparturient bovine neutrophils demonstrating a reduction of myeloperoxidase during transition [abstract]. Experimental Biology Meeting. Paper No. 776.3.