Description: OBJECTIVE: Develop an automated, software-controlled platform that enhances cutting edge methodologies for genome-scale cellular engineering to enable rapid engineering and optimization of new biomanufacturing systems. DESCRIPTION: Current approaches to engineering biology rely on an ad hoc, laborious, trial-and-error process, wherein one successful project often does not translate to enabling subsequent new designs. As a result, the state of the art development cycle for engineering new biological products often takes several years and costs tens to hundreds of millions of dollars (e.g. microbial production of artemisinic acid for the treatment of malaria and the non-petroleum-based production 1,3-propanediol). The impact from these current approaches is that the number of new entrants and innovators into both the commercial and research space is immediately limited few have the expertise, capital and/or time necessary to develop and engineer a new product. Consequently, while progress has been made, we are constrained to producing only a tiny fraction of the vast number of possible chemicals, materials, diagnostics, therapeutics, and fuels that would be enabled by the ability to truly engineer biology. A new approach is needed. To address bottlenecks plaguing the biological design-build-test cycle and to enable more complex design and engineering, DARPA seeks technologies that enhance automation for genome-scale, cellular engineering. These include automated, programmable, affordable, and compact systems capable of running complex bio-engineering processes (e.g. genome engineering at multiple sites across the genome, cell transfection, combinatorial genome assembly, library design, continuous evolution, etc.). Successful approaches will leverage automation software to enable more complex and robust experimental design (e.g. real-time feedback and control) resulting in outcomes and a scale of experimentation that would be difficult to achieve otherwise. Current platforms developed for complex, genome-scale, cellular engineering protocols are often custom-designed and tailored to a specific lab"s expertise and needs. Few of these techniques are automated; the expectation being that others can implement the protocols in their own labs using their own, custom means. Consequently, transformative techniques are limited to the hands of a relative few. This underscores the inherent challenges to engineering biology replicability and reproducibility. There is significant opportunity for the automation of complex cellular engineering, reducing variability between experiments, and increasing the throughput and capabilities of constructing new biological designs. These innovations will introduce new architectures and tools that will form the foundational technology for engineering biology. This solicitation focuses on the development of automated platforms for enhanced, genome-scale, cellular engineering that enable rapid engineering and optimization of biotechnology, including new biologically-based manufacturing systems. Automated platforms should address several or all of the following challenges intrinsic to the dissemination of complex, cellular engineering protocols: reproducibility, replicability, robustness, efficiency of processes, throughput of experiments, and others. In addition, these automated platforms should enable new experimental protocols and designs that would be difficult or impossible to achieve otherwise (e.g. real-time feedback and control). PHASE I: Develop an initial concept design for an automated platform for enhanced genome-scale cellular engineering that enables rapid engineering and optimization of new biomanufacturing systems. Develop detailed analysis of the automated platform"s predicted performance, including detailed performance analyses of each of the component technologies and processes to be integrated. Include analysis of the performance compared to the standard, non-automated protocol and anticipated improvement on speed or complexity of process. Define key component technological milestones and metrics and establish the minimum performance goals necessary to achieve successful execution of the automated platform. Phase I deliverable will include both a technical analysis of the proposed platform and a commercialization assessment. The technical analysis will include: a technical report of experiments supporting the feasibility of this approach; defined milestones and metrics (including minimum performance metrics) for the development and performance of component processes; a detailed design of the proposed automation platform system; and a description of new experimental protocols and designs that would be difficult or impossible to achieve without automation and software control (e.g. real-time feedback and control). The commercialization assessment will include a Phase II proposal that outlines plans for the development, fabrication, and validation of an automated platform for genome-scale, cellular engineering. This proposal should also include a detailed assessment of the potential path to commercialization, barriers to market entry, competitive landscape (if it exists), and collaborators or partners identified as early adopters for the new system. PHASE II: Finalize the design from Phase I and initiate construction and demonstration of a prototype of the automation platform. Demonstrate that each of the components and processes necessary for implementing the genome-scale, cellular engineering protocol are capable of being performed on an automated platform under software control. Establish baseline performance metrics that improve on comparable non-automated and automated competing processes. Provide an experimentally validated performance comparison of the new, automated process to competing SOA processes. Key metrics include (but are not limited to): reproducibility of experiments, efficiency of component processes, throughput of experiments, total cost and total time to reach end goal, performance of final design/product, and amount of human intervention required. Demonstrate new experimental protocols and designs that would be difficult or impossible to achieve otherwise (e.g. real-time feedback and control) and include attendant, relevant metrics of performance. Deliverables of a prototype device and valid test data appropriate for a commercial production path are expected. PHASE III DUAL USE APPLICATIONS: The ability to rapidly engineer and optimize new biologically-based production systems will have widespread utility and applications across the entire biotechnology and pharmaceutical industries including rapid, optimized production of high value chemicals, industrial enzymes, fuels, diagnostics, and therapeutics. These automated platforms would be impactful for industrial biotechnology firms as well as academic, research-scale operations. These platforms could enable the DoD to leverage the unique and powerful attributes of biology to solve challenges associated with production of new materials, novel capabilities, fuels, and medicines while providing novel solutions and enhancements to military needs and capabilities. For example, automated genome-scale cellular engineering platforms will facilitate the design of systems to rapidly and dynamically prevent, seek out, identify, and repair corrosion/materials degradationa challenge that costs the DoD $23B/yr and has no near term solution in sight. REFERENCES: 1) Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM. Programming cells by multiplex genome engineering and accelerated evolution. Nature, vol. 460, p. 894-898 (2009). 2) Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen AC, Gianoulis TA, Goodman DB, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM. 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