National Human Genome Research Institute | National Institutes of Health U.S. Department of Health and Human Services |
1975-77: DNA SequencingSanger and his colleagues, and Maxam and Gilbert developed rapid DNA sequencing methods. Sanger and his colleagues developed a slightly different protocol for sequencing DNA compared with Maxam and Gilbert. Sanger’s method, where a marker attaches to the growing ends of DNA chains, is used most commonly in labs now. In the past, scientists used radioactivity to mark the ends of DNA chains; now, they use colored dyes.
More InformationReferences:Maxam, A.M., Gilbert, W. A new method for sequencing DNA. Proc Natl Acad Sci, 74 (2): 560-4. 1977. [PubMed]
Sanger, F. and Coulson, A.R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Molec Biol, 94: 441-448. 1975. [PubMed] Sanger, F., Air, G.M., Barrell, B.G., Brown, N.L., Coulson, A.R., Fiddes, C.A., Hutchison, C.A., Slocombe, P.M., Smith, M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature, 265(5596): 687-95. 1977. [PubMed]
Last Reviewed: April 17, 2009 |