DNA Microarray Flowchart
Steps in microarray screening
Core services shown in green; user tasks shown in blue
1. Consultation on experimental design - Experimental objective - Number of samples required - Amount of sample required - Protocol for sample isolation - Type of GeneChip required
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5. Sample analysis - Quantification - Quality control by BioAnalyzer - User will be consulted if samples do not meet lab standards
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2. Submission of experimental worksheet - Download MIAME form - Complete Project, Experiment, User, and RNA Sample pages - Submit form to George Poy
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6. Microarray screening - Sample labeling - Hybridization - Signal quantification
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3. Scheduling - Order GeneChips - Schedule run
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7. Data quality assessment - Statistical analysis to generate QC metrics and outlier detection - Summary report and raw data available via EDGE web interface
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4. Preparation of samples - Isolate RNA or DNA - Deliver samples and GeneChips to Core lab (Bldg 8, Rm 1A11) three days prior to run date
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8. Data analysis - Bioinformatic tools Partek Pro and GeneSpring available to user - Additional bioinformatic support available on a collaborative basis; contact Core staff if interested
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Page last updated: May 31, 2009