NIST Standard Reference Database 1C

NIST Peptide Tandem Mass Spectral Libraries with Search Programs (Data Version: 2012, MS Search Version 2.0g, MSPepSearch Version 0.9)

(Libraries are available free of charge at http://peptide.nist.gov.  Note:  Not all libraries are updated each year.)

 

Contents of the Libraries

Library	Date	Instrument	Species	Spectra
Human	05-30-2012	it	H. sapiens	324,352
Human	04-20-2012	qtof	H. sapiens	16,010
Mouse	04-21-2012	it	M. musculus	143,418
Drosophila	04-11-2012	it	D. melanogaster	78,599
C. elegans	05-24-2011	it	C. elegans	67,470
Yeast	04-06-2012	it	S. cerevesiae	92,609
Yeast	04-17-2012	qtof	S. cerevesiae	14,647
E. coli	04-20-2012	it	E. coli	50,369
Rat	04-16-2012	it	R. norvegicus	55,668
Chicken	05-24-2011	it	chicken	3,125
SigmaUPS1	05-24-2011	it	standard	3,029
BSA	04-01-2011	it	Bovine	725

Compilation
· Data for building the libraries come from typical proteomic measurements of tryptic peptides
· Data are collected from internal measurements, public repositories and directly from producers
· UniRef100 protein sequences are used when available to make identifications
· Primary identifications of MS/MS spectra are made by multiple sequence search engines
· Consensus spectra are generated from a minimum of 3 and a maximum of 100 replicates
· Consensus spectra with high levels of impurities have been removed through an automated process
· The libraries are produced in ASCII .msp format, NIST binary folders and SpectraST formats

Library Search Sofware

· The full-featured software MS Search 2.0 is fully compatible with peptide libraries
· A batch search program, MSPepSearch, is also available

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System Requirements: PC with Microsoft® Windows NT® 4.0, Windows® 2000, Windows® XP, Windows Vista®,or Windows® 7; hard disk with 1GB of available space required.

For more information please contact:

 

The Technical contacts for the database:

Stephen E. Stein
Mass Spectrometry Data Center
National Institute of Standards and Technology
Gaithersburg, MD 20899-8320
(301) 975-2505 sstein@nist.gov

Paul Rudnick
Chemical and Biochemical Reference Data Division 
National Institute of Standards and Technology
Gaithersburg, MD 20899
phone: (301) 975-5828 email: paul.rudnick@nist.gov


Keywords: peptide mass spectral library, NIST peptide library, proteomics, peptide fragmentation, library search, library search.