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Human Genome News Archive Edition

Human Genome News, January 1998; 9:(1-2)

 Table 1.  Large DNA Cloning Systems
 
 
YAC
BAC
PAC
HAEC
MAEC
Host
Yeast
Bacteria
Bacteria
Human
Mouse
Genetic Elements
ARS/CEN
F episome
P1 bacteriophage
OriP/EBNA-1
HAEC OriP/EBNA-1
DNA Status
Linear
Circular
Circular
Circular
Circular
Insert size (kb)
100 to 2000
40 to 300
40 to 250
80 to 350
80 to 120
Copy#/cell
1
1
1
~1 to 10
~1 to 10
Amplification
-
-
20x
1000x
(+)
Viral Stock
-
-
+
+
(ND*)
Non-Recombinant
-
+
+
-
(ND)
Chimeras
+
-
-
-
(ND)
Stability
(+ -)
+
+
+
(+)
Major application
STS linkage, contig maps
Contig maps, sequencing
Contig maps, sequencing
Functional analysis
Functional analysis
References
Burke et al. (1987)
Shizuya et al. (1992)
loannou et al. (1994)
Sun et al. (1994)
Kelleher et al. (1997)
 * not determined.
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