Scanning Transmission Electron Microscopy Facility
An Outside User Facility at the Biology Department
News Releases:
Contacts:
Biology Department, Bldg 463
Brookhaven National Lab
Upton, NY 11973-5000
fax: (631) 344-3407
Facility:
STEM
is a custom-built electron microscope optimized for imaging unstained biological
molecules with minimal radiation damage. The group at Brookhaven operates STEM as
a User Facility with partial DOE and partial support
Fee-for-Service
(viewable with Abobe Reader).
Applications:
STEM mass measurements can:
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Determine stoichiometry and homogeneity of a complex
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Compare modified or re-assembled vs. native structures
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Determine filament symmetry from mass per unit length
In addition, STEM is the best instrument for visualizing small metal clusters used as
heavy atom labels.
Samples:
Molecular Machines |
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Flagella |
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Virus Assembly |
DNA/RNA/protein complexes
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Microtubules |
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Heavy Atom Labels |
Lipoproteins |
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Prions |
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Nano Particles |
Alzhiemer’s Filaments |
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Pili |
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Nano Assemblies |
Amyloids |
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Filamentous Viruses |
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Fibrin |
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Spherical Viruses |
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Video:
The role of the STEM and cryo-EM in structural biology is summarized by
Joe Wall in this 3.5 minute
STEM Video
clip viewable with
Real Player.
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Last Modified: March 8, 2010 Please forward all questions about this site to:
Denise Monteleone
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