This Review describes the latest methods for profiling common epitranscriptomic marks, their scale, resolution and ability to quantify. It also discusses remaining challenges.

A lentiviral library expressing Cpf1 guide RNAs and containing target sequences allows high-throughput profiling of highly active guide RNAs and is the basis for cindel, a webtool to predict the activity at any given target sequence.

Stroke is often modeled in rodents by surgically occluding vessels. SIMPLE is an alternative approach that involves the magnet-induced accumulation of nanoparticles. Because of its reversible nature, this method can be used to study both occlusion …

The mechanical properties of tissues can be measured by deforming magnetically responsive microdroplets that are implanted in the tissue. Serwane et al. apply this method to study the mechanical properties of tissues in the living zebrafish embryo.

Avoiding nonspecific binding is essential for performing fluorescence microscopy–based analyses of single molecules tethered to surfaces. A dichlorodimethylsilane–Tween-20–passivated surface provides a useful alternative to the standard …

Benchmarking on synthetic data reveals differences between common RNA-seq alignment software tools, particularly for complex genomic regions.

Simul-seq profiles genomes and transcriptomes by uniquely barcoding DNA and RNA reads from the same material.

InWeb_InBioMap (InWeb_IM for short) is a scored, integrated human protein–protein interaction network resource aggregated from public, experimentally determined protein–protein interactions. The resource enables functional interpretation of …

Standardized driver and effector lines that use optimized GAL4 from a cryophilic yeast species enable bipartite control of transgene expression in Caenorhabditis elegans.