B. Konstantinovic, M. Meseldzija, S. Popovic, Bo. Konstantinovic (2003): Study of resistance to ALS inhibitors in the weed species Echinochloa crus-galli, The BCPC International Congress, Glasgow, Scotland, UK, Vol. 2. pp 771 – 775.
ABSTRACT
The aim of the study was the determination of whether resistance to ALS inhibitors occurred in the weed species Echinochloa crus-galli. Herbicide resistance represents an adaptive phenomenon resulting from repeated use of herbicides with the same mode of action. Resistance determination studies were performed in 2002, and material for the study was collected from different localities in the region of Vojvodina (Serbia), specifically Kamendin, Backi Maglic and Becej. Repeated use of the ALS-inhibiting herbicides, which are used very successfully against dicotyledonous and monocotyledonous weeds, has resulted in the occurrence of resistant biotypes of E. crus-galli. Results obtained from biological studies, whole plant tests and Petri dish bioassays, confirmed the presence of resistant E. crus-galli.
B.Konstantinovic, M.Meseldzija (2002): Determination of triazine-resistant biotypes of Chenopodium hybridum L., 12th Symposium EWRS, Proceedings, Papendal, Arnhem, The Netherlands, pp. 144 – 146.
ABSTRACT
In 2000 and 2001 the occurrence of C.hybridum resistance was studied using petri dish and whole plant bioassays (Clay & Underwood, 1990). Populations of C. hybridum showing resistance were chosen by visual assessment of atrazine efficiency in the localities Backi Maglic, Planta and Kamendin all with a long history of triazine use. A susceptible population collected from an area where no herbicides had been used was used as reference population. In whole plant bioassays, plants were grown from seeds in controlled conditions and sprayed with a range of atrazine rates, e.g. 0.75, 1.0, 1.25, 1.5 and 2.0 kg a.i./ ha. Assessments were performed visually, by recording the number of germinated plants, and by measuring foliage fresh weight. In the Petri dish assays, seed of C. hybridum were germinated on filter paper containing the following range of atrazine concentrations: 0.75, 1.0, 1.25, 1.5 and 2.0 ppm. There were four replications of each treatment. Germination and seedling conditions were recorded at intervals up to 25 days from the onset of the experiment, and root length was also measured .
Key words: Chenopodium hybridum L., bioassays, triazine, resistance.
B Konstantinovic (2001): Determination of triazine resistant biotypes of Setaria viridis, The BCPC Conference Proceedings, Brighton, UK, Vol. 2, pp. 607-612
ABSTRACT
Since 1966 triazine herbicides (ametryn, simazine, atrazine and prometryn) have been widely used in Yugoslavia. This paper investigates the development of resistance to triazine herbicides in Setaria viridis from different localities. Seed of weed species that could be resistant have been collected from different localities in Vojvodina, such as Backa Palanka, Backi Maglic and Becej. Whole plant studies and Petri dish assays were performed during 1999 and 2000. Plants were treated by range of atrazine rates in controlled conditions, including also susceptible, reference population. Seeds were sown in Petri-dishes containing solutions at a range of concentrations of atrazine. Results of both tests indicate the presence of atrazine resistance in S. viridis from Becej locality, which has been treated by triazine herbicides for many years.
Konstantinovic, B., Meseldzija, Maja,Sunjka, Dragana,Konstantinovic. Bo. (2003):
DETERMINATION OF RESISTANT BIOTYPES OF AMARANTHUS RETROFLEXUS L. ON ALS INHIBITORS. 3rd International Plant Protection Symposium at Debrecen University), Debrecen, Hungary, pp. 269-276
ABSTRACT:
The aim of the study was determination of resistance occurrence of the species Amaranthus retroflexus L. on ALS inhibitors. Weed resistance toward herbicides represents phenomenon of these plant species adaptation toward changed environmental conditions and occurs as the consequence of use of herbicides with the same mode of action during several years lasting period. Studies with the aim of resistance determination were performed during 2002, and material for the studies was collected from various sites at Becej locality (Vojvodina). Long lasting use of the herbicides belonging to the group of ALS inhibitors, which are very successfully applied for control of dicotyledonous and monocotyledonous weeds in certain areas lead to occurrence of resistance of the weed species Amaranthus retroflexus L. Results of the study obtained by biological assays, field experiments and Petri dish assays confirmed presence of resistant biotype Amaranthus retroflexus L.
Konstantinovic, B., Meseldzija, Maja, Konstantinovic, Bo. (2004): Determination of Abutilon theophrasti Medic. atrazine resistance. 4th International Weed Science Congress, Abstract book Weed Science Serving Humanity. Durban. Republic of South Africa, pp. 50
ABSTRACT
Long lasting use of herbicides with similar mode of action enabled control of
susceptible weeds, but also spread of resistant ones. Resistance should be
considered as possible cause in cases when herbicides, even after multiple
applications of the recommended rates show no visible effects. The aim of the
work was to study occurrence of Abutilon theophrasti Medic. atrazine
resistance. This triazine herbicide, the inhibitor of photosynthesis in
photosystem II has been widely used in Yugoslavia since 1966. Seeds were
collected during 2002 in various localities of Vojvodina, with a long history
atrazine use. For reference, a susceptible population was collected from an area
that was free of herbicide treatment. During 2002 and 2003, studies were
performed in laboratory by Petri dishes bioassays (Clay and Underwood, 1990),
and whole plant studies (Moss, 1995). Assays were set up in four replications
with a range of atrazine concentrations, e.g. 0.0, 0.5, 0.75, 1.0, 1.25, 1.5,
and 2.0 kg a.i.ha-1. Germination energy assessment, foliage fresh weigh and
plant height were measured after two to eight weeks from the onset of the
experiment. By the comparison of the studied samples with susceptible
standard it was determined that Abutilon theophrasti Medic population from
locality Zabalj evolved atrazine resistance. This means that resistant
biotype must be controlled by using herbicides with alternative modes of action.
Key words: Abutilon theophrasti Medic., atrazine, resistance.
Branko Konstantinovic & Maja Meseldzija (2001): Determination of resistant biotypes of Amaranthus retroflexus L. on triazines, Med. Fac. Landbouww. Univ. Gent, 66/2b, pp 769-774.
ABSTRACT
Considering the fact that since 1966 in our country the mostly produced have been triazine herbicides on the bases of ametryn, simazine, atrazine and prometryn, we have studied resistance of Amaranthus retroflexus from different sites in regard to the above mentioned herbicides. Seed of weed species for which exist possibility of resistance have been collected from different localities in Vojvodina, such as Backa Palanka, Backi Maglic and Becej. Studies were performed during 1999 and 2000 by whole plant studies (Thurnwachter, 1998) and by petri dish assays (Clay, Underwood, 1989). Plants were treated by range of atrazine rates (Atrazin S-50) including also susceptible, referent population. Results indicate atrazine resistance of Amaranthus retroflexus at Backa Palanka and Backi Maglic sites, which have been treated by triazine herbicides in many years lasting period.
Key words: atrazine, herbicides, weeds, resistance, Amaranthus retroflexus