OriGene shRNA Recent Citations

RNA interference-mediated in vivo silencing of FasL as a strategy for the enhancement of DNA vaccine potency, Human Gene Therapy. August 2008, 19(8): 763-773 [sh-Luc(in vivo application)]

Suppression of Sproutys Has a Therapeutic Effect for a Mouse Model of Ischemia by Enhancing Angiogenesis, Koji Taniguchi, Ken-ichiro Sasaki, Kousuke Watari, Hideo Yasukawa, Tsutomu Imaizumi, Toranoshin Ayada, Fuyuki Okamoto, Takuma Ishizaki,Reiko Kato,Ri-ichiro Kohno,Hiroshi Kimura,Yasufumi Sato,Mayumi Ono,Yoshikazu Yonemitsu,and Akihiko Yoshimura, PLoS ONE. 2009; 4(5): e5467 [Spry4(in vivo application)]

MicroRNA-98 and let-7 Confer Cholangiocyte Expression of Cytokine-Inducible Src Homology 2-Containing Protein in Response to Microbial Challenge, J. Immunol., Aug 2009; 183: 1617 - 1624 [CISH]

[6]-Gingerol Suppresses Colon Cancer Growth by Targeting Leukotriene A4 Hydrolase, Cancer Res., Jul 2009; 69: 5584 - 5591 [LTA4H]

Breast Cancer Migration and Invasion Depend on Proteasome Degradation of Regulator of G-Protein Signaling 4, Cancer Res., Jul 2009; 69: 5743 - 5751. [RGS4]

DNA damage-induced phosphorylation of TRF2 is required for the fast pathway of DNA double-strand break repair, Mol. Cell. Biol., Jul 2009; 29: 3597 - 3604 [TRF2]

Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRK, Cellular Signalling, Volume 21, Issue 7, July 2009, Pages 1207-1217 [RALa]

All Citations click here

HuSH-29: Genome-wide shRNA

Double Gene Knockdown Experiment

Scrambled shRNA (GFP Vector)
+
Scrambled shRNA (RFP Vector)

shRNA against RFP (GFP Vector)
+
shRNA against GFP (RFP Vector)

Now available for rat genes

HuSH-29 are pre-designed shRNA with genome wide coverage (human, mouse and rat) that delivers guaranteed knockdown. The same superior design is also offered for other species through Exact-shRNA. A new offering of pGFP-V-RS and pRFP-C-RS vectors enable easy monitoring of transfection efficiency.
(Learn more about HuSH-29...)

Features and Benefits

Pre-designed 29mer targets against Human, Mouse & Rat
Three retroviral vector options:
- pRS: basic retroviral vector without fluorescence
- pGFP-V-RS: pRS with GFP to monitor transfection
- pRFP-C-RS: pRS with RFP as second fluorescence, ideal for double knock down with pGFP-V-RS
5ug of purified plasmid for immediate transfection
Guaranteed successful gene knockdown (≥70%)
Versatile applications: transient or stable transfection or retroviral infection
As low as $595 for a kit (4 gene-specific shRNA and two negative controls)

Technical Support

Our scientists will answer your technical questions from 9AM to 7PM ET. Call 1-888-267-4436

Applications:

Gene silencing through transient or stable transfection
Gene silencing through retroviral transduction
Ideal for shRNA validation by co-transfection of RFP-shRNA with GFP-ORF clones

HuSH-29 shRNA cited for in vivo experiments. Learn more…

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