Jean Celli, Ph.D.
Chief, Tularemia Pathogenesis Section
Tenure-Track Investigator
Tularemia Pathogenesis Section
Dr. Celli received his Ph.D. in microbiology from the University Pierre & Marie Curie, Paris, France, in 1997. After completing postdoctoral training in 2001 in the laboratory of Dr. B. Brett Finlay at the University of British Columbia, Vancouver, Canada, Dr. Celli accepted a research scientist position from the Institut National de la Santé et de la Recherche Médicale (INSERM) to work at the Centre d’Immunologie de Marseille-Luminy, Marseille, France. In 2004, he was recruited to NIH as a tenure-track investigator in the Laboratory of Intracellular Parasites. He is a member of the American Society for Microbiology.
Description of Research Program
The focus of our research is to understand the molecular mechanisms by which intracellular bacterial pathogens circumvent host defense mechanisms and, in particular, survive and replicate within mammalian cells. To study these processes, we use Francisella tularensis and Brucella abortus as model pathogens. Essential to the pathogenesis of these intracellular bacteria is their ability to survive and replicate within macrophages of the host. We are combining microbiology, genomics, and cell biology to identify the mechanisms that allow Francisella and Brucella to evade the degradative pathways and generate an intracellular replicative niche.
Research projects on Francisella tularensis aim to, first, better define the intracellular life cycle of this bacterium inside macrophages, using confocal microscopy and live-cell imaging methodologies to understand the mechanisms of Francisella evasion from intracellular killing and, second, identify Francisella genes required for intracellular survival and replication through transcriptional profiling inside macrophages and mutagenesis approaches. We have characterized Francisella intracellular trafficking in detail and established the intracellular transcriptome of this pathogen during its life cycle within macrophages. Current projects involve the characterization of newly identified gene functions that are upregulated intracellularly.
Brucella abortus resides in a membrane-bound vacuole that traffics along the endocytic degradative pathway before interacting with the host endoplasmic reticulum (ER) to generate an ER-derived organelle that is permissive for replication. Biogenesis of this unique organelle requires functional ER exit sites and is dependent upon the Brucella VirB type IV secretion system, a machinery dedicated to the translocation of bacterial proteins into the host cell. Such proteins are likely to modulate host functions to alter intracellular trafficking of the Brucella-containing vacuole (BCV) and allow bacterial survival. Research projects on Brucella use bacterial genetics, genomics and cell biology to, first, understand the mechanisms of BCV-ER fusion; second, identify proteins translocated by the VirB apparatus and their host cell targets in order to understand the biogenesis of the Brucella replicative organelle at the molecular level; and, third, identify new bacterial factors involved in Brucella intracellular survival.
Graphics
![Scanning electron micrograph of a bone marrow-derived macrophage infected with Francisella tularensis strain LVS for 16h.](https://webarchive.library.unt.edu/web/20090508063207im_/http://www3.niaid.nih.gov/NR/rdonlyres/91F0EBDC-54FC-43C7-B378-F11CF89B22C2/0/Fig1_Celli_Francisella_image.jpg) |
Figure 1. Scanning electron micrograph of a bone marrow-derived macrophage (pseudocolored in blue) infected with Francisella tularensis strain LVS (peusodcolored in yellow) for 24h. After fixation, macrophages are mechanically fractured open to reveal replicating intracellular bacteria. Bacteria are located either in the cytosol or within autophagic vacuoles. Micrograph courtesy of Elizabeth Fischer. |
![Model of intracellular trafficking and survival of Brucella inside macrophages.](https://webarchive.library.unt.edu/web/20090508063207im_/http://www3.niaid.nih.gov/NR/rdonlyres/A1324D02-16C6-4CCF-8D51-5A90A58EAF76/0/LICP_Celli_figure3.jpg) |
Figure 2. Model of intracellular trafficking and survival of Brucella inside macrophages. After phagocytic uptake, intracellular Brucella resides within a vacuole (BCV) that interacts with the endocytic compartment (A), then with endoplasmic reticulum (ER) exit sites (ERES) (B) within the first hours after infection. Such interactions are sustained over time and lead to limited fusion events with the ER (C), ultimately generating an ER-derived organelle permissive for Brucella replication (D). Biogenesis of the replicative organelle requires the Brucella VirB type IV secretion system and Sar1-dependent functions of the ER exit sites. |
![Differential Interference Contrast (DIC) and fluorescence micrograph of a bone marrow-derived macrophage infected with GFP-expressing Brucella abortus.](https://webarchive.library.unt.edu/web/20090508063207im_/http://www3.niaid.nih.gov/NR/rdonlyres/CC03409C-B4FA-49C6-984F-E032BC0280E9/0/celliFig3.jpg) |
Figure 3. (A) Differential Interference Contrast (DIC) and fluorescence micrograph of a bone marrow-derived macrophage infected with GFP-expressing Brucella abortus. Bacteria (green) are seen replicating in the perinuclear area of the cell. (B) Confocal microscopy micrograph of a murine bone marrow-derived macrophage infected with GFP-expressing Brucella abortus for 24 hours. Intracellular Brucella (green) replicate within ER-derived vacuoles (calnexin staining, red) segregated from the endocytic pathway (LAMP-1 staining, blue). |
Research Group Members
![LICP Research Group Members](https://webarchive.library.unt.edu/web/20090508063207im_/http://www3.niaid.nih.gov/NR/rdonlyres/C8F825E2-7C1D-4B36-A897-FE11F21BDB7A/0/LICP_Celli_Jean_group.jpg) |
From left to right, bottom to top: Dedeke Brouwer, Jean Celli, Tregei Starr, Jessica Edwards, Audrey Chong, Henriette Geier, Robert Child, Tara Wehrly. |
Selected Publications
To view a complete listing, visit PubMed.
Chong A, Wehrly TD, Nair V, Fischer ER, Barker JR, Klose KE, Celli J. 2008 The early phagosomal stage of Francisella tularensis determines optimal phagosomal escape and Francisella pathogenicity island protein expression., Infect Immun. 76(12): 5488-99.
Starr T, Ng TW, Wehrly TD, Knodler LA, Celli J. Brucella intracellular replication requires trafficking through the late endosomal/lysosomal compartment. Traffic. 2008 May;9(5):678-94.
Checroun C, Wehrly TD, Fischer ER, Hayes SF, Celli J. Autophagy-mediated reentry of Francisella tularensis into the endocytic compartment following cytoplasmic replication. Proc Natl Acad Sci USA. 2006 Sep 26;103(39):14578-83.
Celli J, Salcedo SP, Gorvel JP. Brucella coopts the small GTPase Sar1 for intracellular replication. Proc Nat Acad Sci USA. 2005 Feb 1;102(5):1673-8.
Celli J, de Chastellier C, Franchini DM, Pizarro-Cerda J, Moreno E, Gorvel JP. Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum. J Exp Med. 2003 Aug 18;198(4):545-56.
Knodler LA, Celli J, Hardt WD, Vallance BA, Yip C, Finlay BB. Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems. Mol Microbiol. 2002 Mar;43(5):1089-103.
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