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| The NIH
Tetramer Facility provides MHC class I and class II tetramer
reagents to the research community worldwide. |
Established in 1999, the NIH Tetramer Facility provides custom
synthesis and distribution of soluble MHC-peptide tetramer reagents
that can be used to stain antigen-specific T cells. These
tetramers are produced at the NIH Tetramer Facility contract
site located at Emory University. Reagents are free to
approved investigators, although requestors must provide the
epitope peptides and pay for shipping. (See Request
Process for more details.)
All reagents are quality controlled (see FACS
Analysis of Stained Cells) and can be labeled with commercially-manufactured
or custom-made fluorophores or
provided in biotinylated monomer form.
While the majority of reagents produced are classical class
I tetramers, the NIH Tetramer Facility also provides human and
mouse CD1d monomers and tetramers and select human and mouse
class II MHC tetramers.
Please note that the NIH Tetramer Facility generally does not
provide tetramers sold by Beckman Coulter Immunomics Operations
(BCI). Please check the list
of Excluded Tetramers before ordering.
Comments or suggestions for
new reagents are welcome.
The NIH Tetramer Facility is supported by a contract from
the National Institute of Allergy and Infectious Diseases with
co-sponsorship by the National Cancer Institute, components
of the National Institutes of Health in the department of Health
and Human Services.
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NIH Tetramer Core Facility
at Emory University
Emory University Vaccine Center
954 Gatewood Road
Atlanta, GA 30329
Phone: 404.727.8117
Give us Feedback...
For information regarding registration or submission of a reagent request, please contact:
Dr. Alison Deckhut Augustine
NIAID Program Officer
National Institute of Allergy and Infectious Diseases,
NIH
augustine@niaid.nih.gov
301.496.7551
For questions about approved or pending requests, please contact:
Dr. Amy Stout
Facility Manager
NIH Tetramer Core Facility
at Emory University
akstout@emory.edu
404.727.8117
For assistance with technical problems, please contact:
Dr. Rick Willis
Technical Director
NIH Tetramer Core Facility
at Emory University
richard.willis@emory.edu
404.727.7215
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