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Regional Methods Initiative: Examination of Enzyme-Linked Immunosorbent Assay (ELISA) for Endocrine Disrupting Compounds (EDCs), Estrogen and Alkyl Phenol Ethoxylates
Project Purpose:
The purpose is to evaluate the reliability and reproducibility of this ELISA technique to analyze environmental samples in different matrices for EDCs. If validated, this technique could be used as a screening method for use in assessing surface water and effluent samples for various EDCs.
Project Description(s):
This study will be a round robin evaluation of ELISA kits done in four phases. Phase I will consist of clean water samples being spiked with estradiol, ethynylestradiol, and alkylphenol ethoxylate and shipped to multiple ELISA users to measure the concentration of each EDC. The concentration of each sample will be simultaneously confirmed by GC-MS and LC-MS methods. Phase II will consist of environmental surface water samples going through the same process and Phase III will consist of a more complex matrix effluent samples. Phase IV will examine different pre-treatment, cleanup options if deemed necessary.
Other Information: This study will be a round robin evaluation of ELISA kits done in four phases. Phase I will consist of clean water samples being spiked with estradiol, ethynylestradiol, and alkylphenol ethoxylate and shipped to multiple ELISA users to measure the concentration of each EDC. The concentration of each sample will be simultaneously confirmed by GC-MS and LC-MS methods. Phase II will consist of environmental surface water samples going through the same process and Phase III will consist of a more complex matrix effluent samples. Phase IV will examine different pre-treatment, cleanup options if deemed necessary.
Contact
Eric Kleiner at kleiner.eric@epa.gov