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Scanning Transmission Electron Microscopy Facility
An Outside User Facility at the Biology Department
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Contacts |
Joseph S. Wall James F. Hainfeld Martha N. Simon Frank E. Kito Beth Yu Lin |
wall@bnl.gov hainfeld@bnl.gov msimon@bnl.gov fkito@bnl.gov bylin@bnl.gov |
tel: (631) 344-2912 tel: (631) 344-3367 tel: (631) 344-3372 tel: (631) 344-3372 tel: (631) 344-3372 |
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Facility | STEM is a custom-built electron microscope optimized for imaging unstained biological molecules with minimal radiation damage. The group at Brookhaven operates STEM as a User Facility with partial DOE and partial Fee-for-Service support. |
Applications |
STEM is a quantitative microscopy, and at BNL it is optimized for structure determination
of biological molecules, complexes, and organelles. Freeze-dried, unstained, unshadowed samples
are imaged in-focus which permits:
1. Mass measurements to: 2. Optimal visualization of metal clusters used as specific heavy atom labels. 3. Simultaneous imaging of DNA, protein, and gold labels in a complex. |
Video |
The role of the STEM and cryo-EM in structural biology is summarized by Joe Wall in this 3:30 minute STEM Video clip viewable with Real Player. |
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Updated 17 Dec 2008 |
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