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SAGE Library Info
New SAGE Library Name: | SAGE_Prostate_carcinoma_CL_LNCaP-C |
Old SAGE Library Name: | SAGE_CPDR_LNCaP-C |
Organism: | Homo sapiens |
Library Keywords: | prostate, carcinoma, cell line, short SAGE, SAGE, non-normalized |
#Total tags: | 46,248 |
#Tags excluding linkers: | 40,029 |
#Unique tags: | 15,561 |
Tissue: | prostate |
Cell/Histology type: | carcinoma |
Tissue preparation: | cell line |
Mutations: | null |
Patient age: | null |
Sex: | null |
Other info: | LNCaP cells were maintained in RPMI 1640 (Life Technologies, Inc., Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Inc., Gaithersburg, MD) and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation, charcoal/dextran stripped androgen-free FBS (cFBS, Gemini Bio-Products, Inc., Calabasas, CA) was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene) SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD). |
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