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pExpress-1 Vector Map
Below is a map of the pExpress-1 vector used in the construction of specific MGCC libraries by
Express Genomics. A brief overview of the propietary method
can be found at pExpress-1 library method overview.
pExpress1 Multiple Cloning Sites and Primer Binding Regions: 641-836
The sequence listed here is the (-) strand.
| | | | | | 700 | |
| M13 pUC Forward 23-Base Sequencing Primer | T7 Promoter Primer |
| 5'-CC | CAGTCACGAC | GTTGTAAAAC | G-3' | 5'- | TAATACGACT | CACTATAGGG | -3' |
5'- | GCAGTTTTCC | CAGTCACGAC | GTTGTAAAAC | GACGGCCAGT | GCCTAGCTTA | TAATACGACT | CACTATAGGG | ACCACTCCTC |
| <------------T7 Promoter------------> | |
| | | <-----------SP6 Promoter------------> |
| GAGGTTAATT | AAGCGGCCGC | CC | ATCCCGGG | AATTCCGGAG | CGAAACTTGT | TCTATTAGTGT | CACCTAAATA |
| XhoI PacI | NotI | | SmaI EcoI RsrII | | 3'-GATATCACA GTGGATTTA-5' |
| | SP6 Promoter Primer |
| 800 | |
| GGCCTAATGG | TCATAGCTCTGT | TTCCTGTGTG | AAATTGTTAT | CCGCT-3' |
| 3'-GGACACAC | TTTAACAATA | GGCGA-'5 |
| M13/pUC 23-Base Sequencing Primer |
|