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pExpress-1 Vector Map

Below is a map of the pExpress-1 vector used in the construction of specific MGCC libraries by Express Genomics. A brief overview of the propietary method can be found at pExpress-1 library method overview.

pExpress1 Multiple Cloning Sites and Primer Binding Regions: 641-836

The sequence listed here is the (-) strand.

						700 •
	M13 pUC Forward 23-Base Sequencing Primer				T7 Promoter Primer
	5'-CC	CAGTCACGAC	GTTGTAAAAC	G-3'	5'-	TAATACGACT	CACTATAGGG	-3'
5'-	GCAGTTTTCC	CAGTCACGAC	GTTGTAAAAC	GACGGCCAGT	GCCTAGCTTA	TAATACGACT	CACTATAGGG	ACCACTCCTC
						<------------T7 Promoter------------>

				cDNA Insert				<-----------SP6 Promoter------------>
	GAGGTTAATT	AAGCGGCCGC	CC		ATCCCGGG	AATTCCGGAG	CGAAACTTGT	TCTATTAGTGT	CACCTAAATA
	XhoI       PacI	NotI			SmaI    EcoI           RsrII			3'-GATATCACA GTGGATTTA-5'
								SP6 Promoter Primer

	800 •
	GGCCTAATGG	TCATAGCTCTGT	TTCCTGTGTG	AAATTGTTAT	CCGCT-3'
			3'-GGACACAC	TTTAACAATA	GGCGA-'5
			M13/pUC 23-Base Sequencing Primer