Harlan Animal Colonies Health Evaluation Summary: 09/24/07
Contract N01-AG-7-2113
RATS Barrier 202B
Animal Facility | Strain | No.Sex | Age | Testing Laboratory/Dates |
---|
Location: Indianapolis, IN | F344/NHsd | 2M/1F | 5 months | University of Missouri |
Barrier/Building: 202B | | 1M/2F | 10 months | MURADIL, Columbia, MO |
| | | | |
| | | | Receipt Date: 7/23/07 |
Ship Date: 7/22/07 | | | | Report Date: 9/14/07 |
Evaluation
NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan. All rats examined were considered to be in a good state of health. |
Serology
Agent | Assay | No. Pos./No. Tested | Comments |
---|
Sendai | MFI | 0/6 | |
RCV/SDAV | MFI | 0/6 | |
Parvoviruses | MFI | 0/6 | |
KRV | MFI | 0/6 | |
H-1 | MFI | 0/6 | |
PVM | MFI | 0/6 | |
M. pulmonis | MFI | 0/6 | |
Reo 3 | MFI | 0/1 | |
TMEV GDVII | MFI | 0/1 | |
MAD | MFI | 0/1 | |
LCM | MFI | 0/1 | |
Hantaan | MFI | 0/1 | |
Bacteriology
Agent | No. Pos./No. Tested | Comments |
---|
Mycoplasma pulmonis | 0/6 | |
Salmonella spp. | 0/6 | Nasopharynx/lung/cecum/uterine cultures |
Pasturella pneumotropica | 0/6 | |
Pasturella spp. | 0/6 | |
Bordetella bronchiseptica | 0/6 | |
Pseudomonas aeruginosa | 0/6 | |
Citrobacter rodentium | 0/6 | |
Corynebaterium kutscheri | 0/6 | |
Klebsiella pneumoniae | 0/6 | |
Klebsiella oxytoca | 0/6 | The bacteria isolated from the nasopharynx and cecum are opportunistic or normal flora of the rat. |
Staphylococcus aureus | 0/6 |
Staphylococcus pneumoniae | 0/6 | |
Streptococcus sp. Group B beta | 0/6 | |
PCR
| No. Pos./No.Tested | Comments |
---|
Mycoplasma pulmonis | 0/6 | |
Heliobacter hepaticus | 0/6 | |
Parasitology
Type and Test | Results | Comments |
---|
Ectoparasites | No. Pos./No. Tested | External parasites were not observed in any rat. |
Test: Direct pelage
| 0/6 |
Endoparasites | No. Pos./No. Tested | Enteric and urinary bladder parasites were not observed in any rat. |
Test(s): Direct, Gross, and Microscopic (intestines & bladder)
| 0/6 |
Pathology
Gross: | None Significant | Overall frequency of lesions in these rats is low. |
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Histologic: | | |
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Lung lymphoid hyperplasia | 6/6 | Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
Harderian gland lymphoid infiltrate | 2/6 |
Nephrocalcinosis | 3/6 |
Interstitial pneumonia | 1/6 |
Myocardial degeneration | 3/6 | |
A few other incidental lesions were also noted. | | |
Availability
Rats obtained from NIA-NIH aging colonies maintained in a maximum security barrier by Harlan in Indianapolis, Indiana. The combined census of these colonies, at the time of the referenced monitoring, is tabulated below: |
Retired Breeder - Male (9-20 months of age) | 179 | |
Virgin - Male (1-31 months of age) | 4,990 | |
Virgin - Female (1-31 months of age)
| 2,709
| |
Total Census:
| 7,876 | |
For information, contact:
Dr. Karla A. Stevens
HARLAN SPRAGUE DAWLEY, INC.
8520 Allison Pointe, #400
Indianapolis, IN 46250
Phone: (317) 806-6060
Fax: (317) 806-6073
Signed by Karla A. Stevens, D.V.M., Principal Investigator
Page last updated Sep 26, 2008