Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Silk Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus vivanus
FDA Seafood List Common Name: Silk Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940818gel02
Unique Web Code: ssh02Srr
Tissue Aliquot Code: lutjviva 003 #2 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558710.50.0482470.58297610.98.64.5587.17.40.0443163
25.219022.80.042111950.3865797.25.75.2190.55.40.040635
35.8530736.90.064544560.62429811.79.25.85306.25.70.0622468
46.5542250.70.057794180.76890714.411.36.55421.27.70.0565865
57.3551862.30.024439480.3288576.24.87.35515.37.90.0235972
68.1564677.60.050692180.6953671310.28.15645.37.80.0500134
78.4571185.50.06874770.82905415.512.28.45711.17.10.0661044
88.6575590.70.055151750.475418.978.65755.24.90.0546349
99.380296.40.060661660.64645212.19.59.3801.96.30.0583046
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.136263.10.02090560.1977320.60.64.13626.15.60.0198294
24.356607.20.17645962.018836.35.94.35660.66.50.176008
35.0817220.60.15759571.9383265.75.08171.67.10.154066
45.17718722.40.089936981.230873.83.65.177186.680.0869619
55.40222627.10.076336650.621641.91.85.402226.54.70.074829
65.74528634.30.0468012100.7432292.32.25.745284.69.60.0437959
75.90631437.60.041985190.6658212.11.95.906316.78.70.0434016
86.05233940.60.50016343.205529.99.46.052338.83.70.491721
96.1435442.40.31103942.084966.56.16.14353.83.90.298801
106.22836944.20.2164751.768355.55.26.228368.54.60.216185
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.30438245.70.025011930.1256910.40.46.304382.92.90.0247916
126.3839547.30.058395630.3551121.116.38395.43.30.0601485
136.462409490.028118480.5788431.81.76.462414.18.10.0403191
146.53242150.40.10537630.5119451.61.56.532420.83.40.085066
156.68644152.80.4907143.86331211.36.686440.64.50.486775
166.77445254.10.42482231.995076.25.86.774451.62.70.421214
176.8746455.60.11204461.260953.93.76.87463.86.40.110611
186.97447757.10.029569740.2221380.70.76.974476.34.20.030065
197.26251361.40.13950951.116883.53.37.262513.24.70.132972
207.757769.10.036488140.7590872.42.27.7581.213.80.0309749
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.85260071.90.2341152.067016.46.17.852600.15.40.214709
227.99862274.50.43174364.0192312.511.87.998623.55.90.383016
239.32880596.40.077405570.9148752.82.79.328804.46.90.0746067
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559511.30.021304190.2998457.35.64.5594.78.60.019756
25.2193230.026336960.2648816.44.95.2192.65.90.0251231
35.8530436.20.046185560.47306111.58.85.85303.96.10.0440946
46.55428510.048854470.54308913.110.16.55426.96.70.0457773
57.3553263.40.01589380.20567353.87.35530.280.0144518
68.15646770.04489270.60720214.711.38.15644.27.50.0458383
78.45713850.065790670.76505218.514.28.45712.96.60.0653092
88.6576090.60.046934750.43247510.588.65759.15.30.0463966
99.380696.10.052448560.53929213.1109.3805.860.0506749
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.049263.10.007934280.1450740.60.64.049298.20.0100325
24.306647.70.095730871.175544.94.64.30663.770.0952153
34.55100120.02276160.2234590.90.94.5599.35.80.0218154
45.01716920.30.12218971.479976.25.85.017169.37.10.118395
55.219623.50.019897180.2576341.115.2195.48.10.0178647
65.35222226.60.045866150.3660811.51.45.352221.84.60.0452654
75.6827833.30.0311526120.6051222.52.45.68274.812.10.0282672
85.8530736.80.046031560.4627271.91.85.85308.76.10.0428834
96.02533740.40.43516432.5134210.59.86.025337.43.30.427501
106.11335242.20.2375241.52056.35.96.113352.23.80.228752
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.20736844.10.1606551.311685.55.16.207367.14.60.160692
126.28938245.80.018282630.1033240.40.46.289381.93.20.0181296
136.36539547.40.032580930.1882160.80.76.365394.430.0348639
146.45341049.20.0313093100.6247682.62.46.453413.49.60.0367052
156.52342250.60.077456540.394531.61.56.523422.23.70.0603288
166.67944353.10.42773543.065612.811.96.679443.54.10.425254
176.76645454.40.33402931.67276.56.766454.32.90.3263
186.87646856.10.068049380.9140723.83.66.876465.67.80.0662313
197.27151862.10.083641440.5755162.42.27.271517.840.0809646
207.3552863.30.014875340.09333270.40.47.35527.63.70.0143978
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.68557669.10.017227460.1671480.70.77.685576.75.80.0163325
227.84559971.80.15438261.48076.25.87.845598.960.138867
238.0262474.80.27454883.363531413.18.02624.87.80.243535
248.4571986.20.021012460.2060970.90.88.45719.15.60.0206055
258.6576191.20.018356350.1653650.70.68.65760.55.30.0177411
269.380596.50.078054870.9300753.93.69.3803.87.20.0730695
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0782530.015963380.2095920.70.64.07826.57.50.0156782
24.319617.30.15327982.018656.86.24.319617.50.151342
35.03616820.10.15064682.201597.46.75.036168.88.30.14993
45.36922126.40.053161350.5387711.81.65.369219.55.40.0563465
55.67827332.70.0455232100.8126792.72.55.678272.310.30.0443033
65.86130436.40.0305772100.543341.81.75.861307.89.90.0309295
76.01533239.70.3492752.963019.99.16.015332.14.70.355403
86.10334841.60.20818651.971986.666.103347.75.30.209257
96.19136443.50.16162451.567055.34.86.191364.35.40.163848
106.28438145.60.01297430.07194130.20.26.284381.83.10.0129145
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.35639447.10.048190830.29808710.96.356393.93.40.0496949
126.444410490.0329171120.8402462.82.66.444415.811.90.0398473
136.50442150.40.074275130.2550790.90.86.504421.53.50.0416707
146.663443530.45508953.6397912.211.16.6634434.60.447172
156.75445454.30.37153632.111787.16.56.754454.13.30.363821
166.87846956.10.093136771.122643.83.46.878467.67.40.0851714
176.98648257.70.029780760.1430030.50.46.986483.45.50.0146613
187.25151461.50.13626941.012283.43.17.251513.64.50.127696
197.66457268.40.0430111242.225257.56.87.664586.5240.052312
207.859270.80.22727141.347724.54.17.85924.40.173468
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.9561473.40.145316102.9984410.19.27.95618.710.50.161671
229.32180796.50.074371270.89778132.79.321805.37.20.0702585
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559210.90.050291190.722545129.24.5592.38.90.0458664
25.219022.60.043394670.4709357.865.2191.16.50.0407843
35.8529835.40.061445950.5779019.67.45.85298.25.30.0618606
46.5543351.50.0555362100.92083115.311.76.554319.50.0544723
57.3552562.40.024321770.2871494.83.77.35526.170.0230624
68.1564576.70.061230680.82191113.610.58.15645.27.80.0593018
78.45715850.080445160.89099514.811.48.45714.26.40.0787698
88.6575990.20.062756150.5844969.77.48.65758.85.40.061326
99.3807960.075883160.75502312.59.69.38075.90.0725439
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 98
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln