Detailed Research Information

Detailed project information for
Study Plan Number 01095

Branch : Fish Health Branch

Study Plan Number : 01095

Study Title : Laboratory Studies on the Effect of Carbon Dioxide to Kill Pathogenic Bacteria in the Aquatic Environment

Starting Date : 06/01/2002

Completion Date : 09/30/2005

Principal Investigator(s) : Starliper, Clifford E. and Watten, Barnaby J.

Primary PI : Starliper, Clifford E.

Telephone Number : (304) 724-4431

Email Address : cstarliper@usgs.gov

SIS Number : 5005334

Primary Program Element : Fisheries and Aquatic Resources

Second Program Element : Endangered and At-Risk Species

Status : Completed

Abstract :
BACKGROUND

Prevention, control and treatment of fish pathogens in water is an important issue facing resource managers. Topics of concern include the translocation of exotics, treatment of incoming hatchery water and treatment of hatchery water effluent. The pathogens of aquatic organisms are primarily transmitted horizontally via the infectious agents entering the water column; this includes pathogens that are recognized and exotic. Therefore, effective prevention of pathogen transmission is based largely on the quality of the water supply and on effectively decontaminating it so it will not subsequently serve as a reservoir to infectious agents. Potentially, there are a variety of groups of people who could be served by the successful results of this study. At many hatcheries, introduction of pathogens via waterborne exposure has been a problem for fisheries resource managers. Water sources may be contaminated with pathogens from indigenous fishes and this water may serve to infect resident hatchery fish. OBJECTIVES: This project will explore the possibility to expand the use of pressurized CO2 for killing of pathogens in water. Dr. Watten has designed and constructed a closed, water-recycling chamber that can be closely controlled and monitored for various water chemistry and quality parameters including the concentration of dissolved CO2. Dr. Starliper offers the techniques to provide and standardize a pathogen to inoculate into the chambers and the methodology to evaluate the response of bacterial pathogens to CO2.

OBJECTIVES

1) adapt the chamber to be used for aseptic work

2) prime the chambers with suitable concentrations of bacteria and apply various exposures of CO2, then evaluate the response.

PHYPOTHESIS TO BE TESTED:

1) The chamber is adaptable for aseptic work.

2) CO2, at a to be determined minimum of pressure is lethal to bacterial pathogens

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11700 Leetown Road, Kearneysville, WV 25430, USA
URL: http://www.lsc.usgs.gov
Maintainer: lsc_webmaster@usgs.gov
Last Modified: October 21, 2002 dwn
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Branch :	Fish Health Branch
Study Plan Number :	01095
Study Title :	Laboratory Studies on the Effect of Carbon Dioxide to Kill Pathogenic Bacteria in the Aquatic Environment
Starting Date :	06/01/2002
Completion Date :	09/30/2005
Principal Investigator(s) :	Starliper, Clifford E. and Watten, Barnaby J.
Primary PI :	Starliper, Clifford E.
Telephone Number :	(304) 724-4431
Email Address :	cstarliper@usgs.gov
SIS Number :	5005334
Primary Program Element :	Fisheries and Aquatic Resources
Second Program Element :	Endangered and At-Risk Species
Status :	Completed
Abstract :	BACKGROUND Prevention, control and treatment of fish pathogens in water is an important issue facing resource managers. Topics of concern include the translocation of exotics, treatment of incoming hatchery water and treatment of hatchery water effluent. The pathogens of aquatic organisms are primarily transmitted horizontally via the infectious agents entering the water column; this includes pathogens that are recognized and exotic. Therefore, effective prevention of pathogen transmission is based largely on the quality of the water supply and on effectively decontaminating it so it will not subsequently serve as a reservoir to infectious agents. Potentially, there are a variety of groups of people who could be served by the successful results of this study. At many hatcheries, introduction of pathogens via waterborne exposure has been a problem for fisheries resource managers. Water sources may be contaminated with pathogens from indigenous fishes and this water may serve to infect resident hatchery fish. OBJECTIVES: This project will explore the possibility to expand the use of pressurized CO2 for killing of pathogens in water. Dr. Watten has designed and constructed a closed, water-recycling chamber that can be closely controlled and monitored for various water chemistry and quality parameters including the concentration of dissolved CO2. Dr. Starliper offers the techniques to provide and standardize a pathogen to inoculate into the chambers and the methodology to evaluate the response of bacterial pathogens to CO2. OBJECTIVES 1) adapt the chamber to be used for aseptic work 2) prime the chambers with suitable concentrations of bacteria and apply various exposures of CO2, then evaluate the response. PHYPOTHESIS TO BE TESTED: 1) The chamber is adaptable for aseptic work. 2) CO2, at a to be determined minimum of pressure is lethal to bacterial pathogens
For More Information :
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U.S. Department of the Interior \|\| U.S. Geological Survey 11700 Leetown Road, Kearneysville, WV 25430, USA URL: http://www.lsc.usgs.gov Maintainer: lsc_webmaster@usgs.gov Last Modified: October 21, 2002 dwn Privacy Policy and Disclaimers \|\| FOIA \|\| Accessibility