Ethnobotanical Leaflets 12: 912-15. 2008. Antimicrobial
Studies of Triterpenoid Fractions from Myxopyrum
smilacifolium Blume Sudharmini, D.* and Ashalatha
S. Nair Department
of Botany, *email
: sudhachandrababu@yahoo.com Issued
Abstract
Triterpenoids isolated from Myxopyrum
smilacifolium leaf showed
presence of ursolic acid (0.175mg/g). The triterpenoids showed antimicrobial
activity in gram positive bacteria and Candida sps. Keywords: Myxopyrum
smilacifolium,Ursolic acid, HPLC, Antimicrobial activity. 1.
Plant Myxopyrum smilacifolium (Oleaceae) is an important medicinal plant
widely used in indigenous system of medicine in 2.
Use in traditional medicine
The leaves are useful in vitiated conditions
of kapha and vata, cough, asthma, rheumatism, cephalalgia, nostalgia, fever,
otopathy, neuropathy and cuts and wounds. Roots are useful in scabies and
prurigo in children [1] 3.
Previously
isolated classes of constituent Iridoid glucosides, namely myxopyroside [2]. 4. New constituents
Isolation of triterpenoid fractions by silica gel thin layer
chromatography using the mobile phase
petroleum: dichloroethylene: acetic acid (50:50:0.7) [3]., HPLC analysis
of ursolic acid and antimicrobial activity by using filter paper disc
diffusion method [4, 5]. 5. Used materials Ursolic acid standard was collected
from Sigma, Aldrich, 6. Results Reported in figure1 and 2,
and Table1. 7. Conclusions Ursolic acid is found in the leaves of Myxopyrum smilacifolium. All the tripterpenoids from the
plant showed antimicrobial activity against the gram positive bacteria
Staphylococcus aureus, Bacillus subtilis, Candida albicans and C. glabrata. The
antifungal activity was similar to the chemical fungicide. Acknowledgements The first author is thankful to
University Grant Commission (UGC), Government of India, References [1] Varier P S, Indian medical
plants: a compendium of 500 species, vol.4. [2] Franzyk H, Jensen S R, Olsen C E. J Nat
Prod 2001; 64:632-633. [3] Harborne J B. Phytochemical Methods: A
guide to modern technique of plant analysis. 3rd ed. [4]
Liu C H., Liu Y. China journal of
Chinese materia medica 2002;
27(12):916-918. [5]
Benson H J. Microbiological applications. 5th ed. Table 1. Antimicrobial activities of isolated triterpenopid fractions of Myxopyrum smilacifolium.
Values are the mean of three
replicates. TTR*- Triterpenoid a Including the diameter of the filter paper
disk (6mm).
Fig 1. Ursolic acid HPLC profile at 205nm.
Fig 2. Myxopyrum smilacifolium fraction
(hRf 20) HPLC profile. |