![]() |
|||||||||||||||||
|
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||||||||
|
![]() |
![]() |
EID Home | Ahead of Print | Past Issues | EID Search | Contact Us | Announcements | Suggested Citation | Submit Manuscript PDF
Version | Download Adobe
Acrobat | Comments |
|
![]()
|
![]() |
Perspective Detecting Bioterror Attacks by Screening Blood Donors: A Best-Case AnalysisEdward H. Kaplan,*Christopher A. Patton,† William P. FitzGerald,†
and Lawrence M. Wein‡
The health and economic consequences of an extensive bioterror attack could be severe (1–5); thus, early detection of an otherwise silent bioterror attack is of obvious importance (6). Ongoing developments in rapid testing for potential bioterror agents (7–10) led us to consider whether screening blood donors to detect a bioterror attack with the most feared bioterror agents (11) could prove useful. The rationale for screening blood donors rests is twofold. First, blood donors are numerous, and donations are uniformly spread over time and throughout the population. In the United States, approximately 13.9 million blood donations are made each year (12); thus, the annual number of donations roughly equals 5% of the 286 million population. Second, in the absence of specific information regarding how such an attack might target the population, we can assume that blood donors are as likely to be infected in a bioterror attack as nondonors. In a sizeable attack, infected donors might donate blood before their infections have been detected medically. Screening donated blood for bioterror agents could therefore serve to detect an attack sooner than would otherwise be possible. However, the cost of screening donations is proportional to the number of donations tested, in addition to the resources expended investigating false alarms. To investigate these issues, we developed a model for bioterror attack detection under assumptions favorable to donor screening, for if such best-case assumptions fail to justify screening donors, more realistic assumptions will also. In particular, we initially assume that the screening test used is perfectly specific, which removes the possibility of false alarms, and compare the time required to detect an attack through donor screening to the incubation periods for various bioterror agents to see whether donor screening leads to more rapid detection than simply observing symptomatic cases. We then consider tests with imperfect specificity, examine the false-alarm rate that would result from donor screening, and compare this rate to the true-positive rate for blood donations. MethodsThough blood tests with the ability to detect agents such as smallpox virus or Francisella tularensis within days after infection do not exist at present, research to develop such sensitive tests is under way (7–10). To analyze whether screening donors might meaningfully shorten the time required to detect an attack were such tests available, we developed a probabilistic model that joins the workings of a screening test, blood donation, and epidemic spread under assumptions that deliberately favor attack detection through donor screening (see Appendix). In the model, the sensitivity of a screening test is determined by a (random) window period W with mean ω days that must transpire before a person infected at time 0 can be detected as infected. Test sensitivity thus depends on the time from infection until testing. Though the model can accommodate any probability distribution desired, we take W to follow an exponential distribution in our examples, an assumption that favors early detection (since the exponential likelihood is maximized at W=0, that is, no detection delay, and declines as W increases). We assume initially that the screening test is perfectly specific, though we will relax this assumption later. A bioterror attack at time 0 infects I(0)=Np persons in a population of size N (where p is the fraction of the population initially infected). We assume that everyone in the population has the same probability p of infection due to the attack, that is, the attack does not target the population in a manner that would make blood donors more or less likely to be infected than nondonors. Given that the total number of blood donations over time results from the independent actions of individual blood donors, the aggregate number of blood donations over time was modeled as a Poisson process (13) with rate l=kN, where k is the mean number of blood donations per person per unit of time. If the agent used in the attack is contagious, secondary infections spread according to an epidemic model, governed by a reproductive number R0 (number of secondary infections per initial index case) and an exponentially distributed duration of infectiousness with mean r-1. To favor donor screening, we deliberately exclude an explicit latent period (during which an infected person is not infectious). These assumptions imply that infections in the population will grow exponentially with rate (R0 – 1)r postattack (14), an assumption that further favors donor screening as the number of blood donors who are infected (and by ignoring latent periods, infectious) will grow exponentially at the same rate, leading to earlier detection via donor screening than would occur otherwise. We assume that the attack is detected once a single infected donation tests positive for infection with a bioterror agent, another assumption favorable to donor screening, which enables us to derive the probability distribution of the time required to detect a bioterror attack of a given magnitude. However, to demonstrate the extent to which we have “stacked the deck” in favor of blood donor screening, we relax the assumption of perfect test specificity for noncontagious agents. We assume fixed attack rates and disaster response and recovery periods, which together determine the fraction of time during which infected donations can occur. This assumption allows us to model the rate of false alarms per unit of time and compare this to the rate of true-positive alarms. Results
For initial attacks ranging from 100 to 1,000 infections, Figure 1 shows the probability distribution of the attack detection delay for a noncontagious agent that would result from using a blood-screening test able to detect infections an average of ω = 3 days after infection (an optimistic assumption, given that such tests do not exist at present), assuming that blood donations arrive at rate k = 0.05 per person per year, the average rate for blood donation in the United States (12). The results are not encouraging: for an attack that infects 100 persons, the chance of detecting the attack through blood donor screening within 25 days is 26%; even for a large attack that infects 1,000 persons, the median time to detect the attack is 8 days. Figure 2 (solid curve) shows the mean delay in attack detection as a function of the initial attack size for a noncontagious agent. For an initial attack that affects 1,000 persons, the mean time to detection is 10 days, while for an attack that affects 100 persons, the mean time to detection is 76 days. In most infected persons, symptoms would develop during this period, leading to earlier detection of an attack than blood donor screening would allow, even when potential delay from misdiagnosis or failure to recognize symptoms is accounted for (Table 1; compare to incubation times from infection through symptoms for Bacillus anthracis and Clostridium botulinum, two noncontagious agents). That we have deliberately made assumptions favorable to blood donor screening strengthens this finding, for the actual time required to detect an attack by means of donor screening would be longer than reported above. If we also assume that ω=3 days and k=0.05 per person per year, Figure 3 shows the distribution of delays in attack detection that would result from a contagious agent characterized by R0=3 and r-1=14 days (parameters suggestive of smallpox [3,11,15] and perhaps Ebola virus [11]). Because additional infections are transmitted to susceptible persons, the probability of detecting an attack within any given period is greater than for a noncontagious agent. Consequently, for a given initial attack size, the attack detection delay distribution is shorter for a contagious agent, as is clear from Figure 3. However, symptoms would develop in many infected persons, and such infections would be recognized before blood donor screening would uncover an attack. Under our best-case assumptions, an attack that initially infects 100 persons would still require 15 days on average before donor screening would detect the attack, while an initial attack infecting 1,000 persons would require 6 days until detection on average (Figure 2). Treating the range of incubation times from infection through symptoms (Table 1) as 99% probability intervals from agent-specific lognormal distributions, in the case of smallpox one would expect to see five symptomatic cases after 7 days, while more than half of those initially infected with Ebola virus would progress to symptoms within 1 week. The incubation times for plague and tularemia are much shorter (Table 1), but even after increasing r to compensate for this in our model many of those infected would exhibit symptoms before the bioterror event was detected through tests of the blood supply (results not shown). Again, considering that we have made assumptions that favor donor screening—that the test has an exponentially distributed window period that detects infection after 3 days on average, that donor screening detects the attack after the first donor tests positive, that there is no latent period from infection through infectiousness, and that a postattack epidemic grows exponentially—donor screening as a method of attack detection does not seem competitive with simple observation of symptomatic case-patients. Until now, we have assumed that screening occurs with perfect specificity, which eliminates false-positive results as a consequence. However, if false-positive test results can occur, they will occur frequently. Table 2 reports the false-alarm rates that would occur for tests of different specificities for a noncontagious agent, if one assumes that all 13.9 million annual blood donations are tested, that on average one bioterror attack takes place per year (a rate all would agree is unrealistically high), that on average 1 month is required to respond to and recover from an attack (so infected donations can occur for up to 1 month after an attack), and that each attack infects 1,000 persons. Even with 99.99% specificity, an average of 1,390 false-positive results would occur per year; at 99% specificity, the average would be 139,000 false-positive results per year. In addition to the resources wasted in investigating so many false alarms, a “crying wolf” mindset could diminish the attention paid to all screening test results, increasing the chance of missing a true-positive test result. That this latter possibility could well occur seems clear because with the attack rate and duration of response and recovery assumed above, one would expect only 3.7 donations with true-positive results each year (again, presuming an exponentially distributed window period with mean w= 3 days). Also, though lowering the attack rate below one per year to more realistic levels would have no effect on the false-positive rate, the number of donations with true-positive results would fall. Similarly, reducing the duration of the postattack response and recovery during which infected donations can still occur would have essentially no impact on the false-positive rate, while again lowering the number of donations with true-positive results. ConclusionWe have argued that even under assumptions deliberately favorable to blood donor screening, an attack was unlikely to be detected earlier through donor screening than from observing symptomatic case-patients. We have also shown that imperfect test specificity could overwhelm the blood collection system with false-positive results. In addition, the costs of screening apply to all blood donations tested: even if the cost of screening were as low as an incremental $10 per test, screening all blood donations in the United States to detect a bioterror attack would cost an additional $139 million per year at current donation rates. Total costs would be even higher when the resources that would be expended investigating false-positive results are considered. For all of these reasons, blood donors should not be screened for bioterror agents for the purpose of detecting a bioterror attack.
AppendixWe consider a single bioterror attack that infects a proportion p of the population at time 0. To model test sensitivity, we presume that a blood test administered to a person t days after becoming infected will test positive for infection with probability FW(t), where W refers to the window period of the test. In our examples we assume that W follows the exponential distribution with mean ω days, that is, FW(t)= 1 – e-t/ω, though the model allows assessment for any window period distribution. We set ω=3 days in our examples. The probability that a randomly selected member of the population would test positive t days after the attack is then given by where as explained following equation 8 below. In equation (2), R0 is the reproductive number specifying the number of secondary infections transmitted by an initially infected individual early in the outbreak, while r-1 is the mean duration of infectiousness (14). We set R0=3 and r-1=14 days in our contagious examples, parameters suggestive of smallpox (3,15), while results for attacks with noncontagious agents are obtained by setting R0=0. Note that π(t) is proportional to p, the fraction of the population initially infected in the attack. Due to the superposition of many individually arriving donors (13), we assume that in the aggregate, blood donations occur in accord with a Poisson process with rate l per unit of time. We set l =kN for some constant k, that is, the blood donation rate is proportional to the size of the population (k=0.05 to represent the average U.S. donation rate [12] in our examples). We further assume that donors are no more or less likely to have been infected than nondonors. The number of blood donations that would test positive within time τ of the attack then follows a Poisson distribution with mean Note that since π(t) is proportional to p while l is proportional to N, ρ(τ) is proportional to I(0)=Np, the initial attack size. Thus the ability to detect a bioterror attack by means of blood donor screening when blood donation occurs at a rate proportional to the population is directly related to the initial number of persons infected in the attack, independently of the size of the population. The probability that at least one blood donation would test positive and detect the attack within τ days is given by while the expected time required to detect such an attack equals because the expected value of a nonnegative random variable equals the integral of its survivor function, as is well-known. Since ρ(τ) is proportional to the initial attack size, the probability of detecting an attack within any fixed time interval increases with the initial attack size, while the expected time required to detect an attack decreases with the size of the attack. In the event of an attack at time 0 with a contagious agent, we approximate the progress of the resulting epidemic with the standard model where N is the population size, and is the disease transmission rate (14). Persons infected in this model immediately become infectious and remain so for r-1 time units on average; thus; no latent period occurs during which a person is infected but not infectious. Early in the epidemic we have N–1(t)≈N, which as usual leads to exponential growth in the number of infections as Note that the per-capita transmission of infection before the detection
of the attack in this model is given by The sensitivity of the attack detection delay to the parameters of this model can be determined directly from the mathematics above. To summarize, the time to detect an attack via blood donor screening will decrease if, ceteris paribus, any of the following parameters increase: the initial number of infections, I(0), the per capita blood donation rate (k), the reproductive number (R0), and the disease progression rate (r). Increasing the mean window period of the screening test (ω) would lengthen the time required to detect an attack. The screening test employed is perfectly specific in the analysis above, which obviates the problem of false alarms by assumption. We now relax the assumption of perfect specificity and instead assume that an uninfected donation will test negative with probability s, where s is the specificity of the test. With this new assumption, uninfected donations will test positive with probability 1−s, which leads to false-positive results. To compare false-positive and true-positive rates for noncontagious agents, we adopt an alternating renewal process model (13) of bioterror attack and recovery (a similar analysis could be conducted for contagious agents, but little insight can be gained from doing so). Under normal circumstances, we assume that attacks occur at a mean rate of a per unit time. Once an attack occurs, we assume that δ time units are required for response and recovery (clearly δ would depend upon the time required to detect an attack, which in turn could be influenced by donor screening, but this effect is minor and not essential for the main results reported). Infected donations can only occur during the response and recovery period, while to simplify the analysis, we presume that no further attacks ensue during the recovery period (indeed, multiple attacks could simply be modeled within this framework as one larger attack). Again for simplicity, we further assume that blood donations occur at the constant rate l= kN over time, and that any attack infects a fraction p of the population. With these assumptions, it follows immediately that the fraction of time occupied by response and recovery, which coincides with the fraction of time during which infectious donations can occur, is given by It follows that the false-alarm rate, FAR (i.e., the mean number of noninfected donations that falsely test positive), is equal to for all donations that test positive do so falsely under normal circumstances, while during the response and recovery period, a fraction (1−p) of donations will be noninfected, and of these (1−s) will falsely test positive. To obtain a simple formula for the true-positive donation rate, note first that the overall attack rate per unit time is given by because, by assumption, attacks do not occur during the response and recovery period. Since ρ(δ) ) infected donations will occur on average during the response and recovery period (where ρ(δ) ) is given by equation [3]), the overall true-positive donation rate (TPDR) is given by In the text, we report results for δ=1 month and References
|
|
||||||||||||
|
||||||||||||
![]() |
![]() |
|
![]() |
![]() |
EID Home | Top of Page | Ahead-of-Print | Past Issues | Suggested Citation | EID Search | Contact Us | Accessibility | Privacy Policy Notice | CDC Home | CDC Search | Health Topics A-Z |
![]() |
![]() |
![]() |
![]() |
This page posted July
14, 2003 |
![]() |
![]() |
![]() |
![]() |
Emerging
Infectious Diseases Journal |
![]() |
![]() |