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A Model for Genetic Diversity Aquatic Insects of the Mobile/Tensaw River Delta
EPA Grant Number: R827072C019Subproject: this is subproject number 019 , established and managed by the Center Director under grant R827072
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Alabama Center For Estuarine Studies (ACES)
Center Director: Shipp, Robert L.
Title: A Model for Genetic Diversity Aquatic Insects of the Mobile/Tensaw River Delta
Investigators: Brockhouse, Charles L. , McCreadie, John W.
Institution: University of South Alabama
EPA Project Officer: Perovich, Gina
Project Period: October 1, 2001 through December 31, 2003
RFA: Alabama Center For Estuarine Studies (ACES) (1999)
Research Category: Targeted Research
Description:
Objective:The objective of this research project is to identify a limited number of aquatic insects that can serve as model species for genetic diversity studies of the Delta region and similar aquatic habitats. Specific objectives are: (1) collection of samples of four populous, widespread, and highly vagile aquatic insect species; (2) development or adaptation of DNA extraction techniques for each candidate species; (3) identification of 10 or more polymorphic nuclear DNA loci within each candidate species; and (4) preliminary assessment of population structures within the Delta. The results of this genomic inventory, in addition to results generated from companion studies, will be used as a springboard to apply for larger grants (e.g., U.S. Environmental Protection Agency, National Science Foundation) for a complete species inventory and an extensive genomic documentation of Delta insects (companion studies: The Paradox of the Delta, John W. McCreadie and Peter H. Alder, ACES-funded project, May 2000; Total Insect Bio-inventory Project of the Mobile/Tensaw Delta, John W. McCreadie and Peter H. Alder, ACES-funded study, August 2001).
The ideal characteristics for a model species include: high genetic diversity at several loci, good vagility to eliminate the possibility of localized inbreeding through low dispersal levels, significant aquatic stages, significant population sizes to avoid natural/historical causes of inbreeding and to facilitate collecting, and widespread populations throughout the Delta region. Ideally, the species will represent different trophic levels for potential use in biomonitoring studies. The successful identification of model species in this project will lead to subsequent projects, including taxon-specific gene flow studies and biomonitoring of impacted regions.
Supplemental Keywords:genetic diversity, genomic inventory, water, water use, aquatic ecosystem, coastal ecosystem, coastal environments, environmental chemistry, chemistry, Alabama, AL, aquatic insects, aquatic habitats, DNA, genetic diversity, gene flow studies, biomonitoring, impacted regions, Ranatra nigra, water scorpion, Pelocoris femoratus, nocorid water beetle, Erythemis simplicicolis, dragon fly, polymerase chain reaction, PCR, Escherichia coli, MutS protein, DNA extraction techniques, gel electrophoresis, DNA cloning. , Ecosystem Protection/Environmental Exposure & Risk, Water, Geographic Area, Scientific Discipline, RFA, ECOSYSTEMS, Ecosystem/Assessment/Indicators, exploratory research environmental biology, Restoration, Chemical Engineering, Aquatic Ecosystem Restoration, estuarine research, Ecology, Aquatic Ecosystems & Estuarine Research, Terrestrial Ecosystems, Aquatic Ecosystem, Chemistry, Ecological Indicators, Environmental Chemistry, Aquatic Ecosystems, Monitoring/Modeling, State, water quality, Mobile Delta, genetic diversity, plant-animal interactions, aquatic plants, aquatic insects, adaptation, land use, Alabama (AL), anthropogenic impact, watersheds, coastal environments, coastal ecosystem, ecosystem, estuaries, water use, biodiversity
Progress and Final Reports:
2002 Progress Report
Main Center Abstract and Reports:
R827072 Alabama Center For Estuarine Studies (ACES)
Subprojects under this Center:
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827072C001 Fluorescent Whitening Agents As Facile Pollution Markers In Shellfishing Waters
R827072C002 Red Snapper Demographics on Artificial Reefs: The Effect of Nearest-Neighbor Dynamics
R827072C003 Stabilization of Eroding Shorelines in Estuarine Wave Eliminates with Constructed Fringe Wetlands Incorporating Offshore Breakwaters
R827072C004 Interaction Between Water Column Structure and Reproduction in Jellyfish Populations Of Mobile Bay (SGER)
R827072C005 Effects of Variation in River Discharge and Wind-Driven Resuspension on Higher Trophic Levels in the Mobile Bay Ecosystem
R827072C006 Results of Zooplankton Component
R827072C007 Benthic Study Component
R827072C008 A Preliminary Survey of Macroalgal and Aquatic Plant Distribution in the Mobile Tensaw Delta
R827072C009 Fisheries-induced changes in the structure and function of shallow water "nursery habitats": an experimental assessment
R827072C010 Effects Of Variation in River Discharge and Wind-Driven Resuspension on Lower Trophic Levels of the Mobile Bay Ecosystem
R827072C011 Evaluation of Alabama Estuaries as Developmental Habitat for Juvenile Sea Turtles
R827072C012 Effects of Salinity Stress on Natural and Anthropogenically-Derived Bacteria in Estuarine Environments
R827072C013 The Role of Land-Use/Land-Cover and Sub-estuarine Ecosystem Nitrogen Cycling in the Regulation of Nitrogen Delivery to a River Dominated Estuary; Mobile Bay, Alabama
R827072C014 Environmental Attitudes of Alabama Coastal Residents: Public Opinion Polls and Environmental Policy
R827072C015 Synthesis and Characterization of an Electrochemical Peptide Nucleic Acid Probe
R827072C016 Determinants of Small-Scale Variation in the Abundance of the Blue Crab Callinectes Sapidus
R827072C017 Effects of Estrogen Pollution on the Reproductive Fitness of the Gulf Pipefish, Syngnathus scovelli
R827072C019 A Model for Genetic Diversity Aquatic Insects of the Mobile/Tensaw River Delta
R827072C020 Evaluating Trophic Processes as Indicators of Anthropogenic Eutrophication in Coastal Ecosystems: An Exploratory Analysis
R827072C021 Effects of Anthropogenic Eutrophication on the Magnitude and Trophic Fate of Microphytobenthic Production in Estuaries
R827072C022 Characteristics of Ship Waves and Wind Waves in Mobile Bay
R827072C023 Methods Comparison Between Stripping Voltammetry and Plasma Emission Spectroscopy for Metals in Mobile Bay
R827072C024 Changes in Water Conditions and Sedimentation Rates Associated With Construction of the Mobile Bay Causeway
R827072C025 Cold-Induced Hibernation of Marine Vibrios in the Gulf of Mexico: A Study of Cell-Cell Communication and Dormancy in Vibrio vulnificus
R827072C026 Holocene Sedimentary History of Weeks Bay, AL: Human and Natural Impacts on Deposition in a Gulf Coast Estuary
R827072C027 Shelter Bottlenecks and Self-Regulation in Blue Crab Populations: Assessing the Roles of Nursery Habitats and Juvenile Interactions for Shelter Dependent Organisms
R827072C028 Predicting Seagrass Survival in Nutrient Enriched Waters: Toward a New View of an Existing Paradigm
R827072C029 DMSP and its Role as an Antioxidant in the Salt Marsh Macrophyte Spartina alterniflora
R827072C030 A Preliminary Survey of Aerial and Ground-Dwelling Insects of the Mobile/Tensaw Delta
R827072C031 Natural Biogeochemical Tags of Striped Mullet, Mugil cephalus, Estuarine Nursery Areas in the North Central Gulf of Mexico
R827072C032 Resolution of Sedimentation Rates in Impacted Coastal Environments Using 137Cs and 210Pb Markers: Dog River and Fowl River Embayments
R827072C033 Investigation of the Use of Pulse Amplitude Modulated (PAM) Fluorometry as an Indicator of Submerged Aquatic Vegetation Health in Mobile Bay
R827072C034 Influence of Invasive Plant Species in Determining Diversity of Aquatic Vegetation in the Mobile-Tensaw Delta
R827072C035 The Influence of Shallow Water Hydrodynamics on the Importance of Seagrass Detritus in Estuarine Food Webs
R827072C036 Food Web Interactions, Spatial Subsidies and the Flow of Energy Between the Mobile Bay Delta and Offshore Waters: A SGER Proposal to the Alabama Center for Estuarine Studies
R830651C001 Meteorological Modeling of Hurricanes and Coastal Interactions: A Stability Study For Vertical Pressure Levels
R830651C002 Characterization of Glycoprotein Cues Used by the Parasitic Rhizocephalan Barnacle Loxothylacus texanus To Identify Its Blue Crab Host, Callinectes sapidus
R830651C003 Survey of Diamondback Terrapin Populations in Alabama Estuaries
R830651C004 An Assessment of Environmental Contaminant Levels in Water and Dragonfly Larvae Tissues from the Mobile/Tensaw Delta