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Microarray System for Contaminated Water Analysis
EPA Grant Number: X832541C001Subproject: this is subproject number 001 , established and managed by the Center Director under grant X832541
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Center for Environmental and Energy Research (CEER)
Center Director: Earl, David A.
Title: Microarray System for Contaminated Water Analysis
Investigators: DeRosa, Rebecca , Cardinale, Jean , Thatcher, Ryan
Institution: Alfred University
EPA Project Officer: Krishnan, Bala S.
Project Period: September 1, 2006 through August 31, 2007
RFA: Targeted Research Center (2006)
Research Category: Targeted Research
Description:
Objective:Our previous study, on a functionalized glass surface to covalently immobilize antibodies, was conducted to produce a more efficient means of water contamination analysis. The current methods used to detect organisms in water require up to 4 days to produce results that are indicative of only a small number of potential contaminates. Enzyme linked immunosorbent assays (ELISA) can use immunological methods to detect a wide range of biomolecules in less time than the current methods. However, traditional ELISAs produce a significant amount of solid waste and require many high-purity antibodies. The costs incurred and biologically- contaminated waste produced by ELISAs have led to the production of a smaller alternative called a microarray. Microarrays use immobilized biomolecules (proteins, lipids, antibodies, and carbohydrates) to detect conjugate biomolecules in solution. Multiple types of biomolecules can be printed onto a single solid glass or polymer substrate to detect multiple conjugate molecules in a single test. Our previous work focused on modification of a glass substrate to increase the surface area available for immobilization and the means by which antibodies were bound to the surface. Covalent immobilization of the antibodies instead of hydrophobic binding is one method that can optimize the binding concentration of antibodies onto the substrate. Our results indicated that surface modifications we made to glass substrates successfully immobilized antibodies. However, the immobilized antibodies denatured, and did not retain their functionality after the immobilization process. Stabilization of the antibodies structure is a potential solution to prevent the denaturing caused during immobilization.
Publications and Presentations:Publications have been submitted on this subproject: View all 3 publications for this subproject | View all 44 publications for this center
Journal Articles:Journal Articles have been submitted on this subproject: View all 1 journal articles for this subproject | View all 7 journal articles for this center
Supplemental Keywords:ELISA, lab on a chip, water contaminants, glass surface modification, protein stabilization, gluteraldehyde, trehalose,
Progress and Final Reports:
Final Report
Main Center Abstract and Reports:
X832541 Center for Environmental and Energy Research (CEER)
Subprojects under this Center:
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R828737C001 Environmental Impact of Fuel Cell Power Generation Systems
R828737C002 Regional Economic and Material Flows
R828737C003 Visualizing Growth and Sustainability of Water Resources
R828737C004 Vibratory Residual Stress Relief and Modifications in Metals to Conserve Resources and Prevent Pollution
R828737C005 Detecting and Quantifying the Evolution of Hazardous Air Pollutants Produced During High Temperature Manufacturing: A Focus on Batching of Nitrate Containing Glasses
R828737C006 Sulfate and Nitrate Dynamics in the Canacadea Watershed
R828737C007 Variations in Subsurface Denitrifying and Sulfate-Reducing Microbial Populations as a Result of Acid Precipitation
R828737C008 Recycling Glass-Reinforced Thermoset Polymer Composite Materials
R828737C009 Correlating Clay Mineralogy with Performance: Reducing Manufacturing Waste Through Improved Understanding
R830420C001 Accelerated Hydrogen Diffusion Through Glass Microspheres: An Enabling Technology for a Hydrogen Economy
R830420C002 Utilization of Paper Mill Waste in Ceramic Products
R830420C003 Development of Passive Humidity-Control Materials
R830420C004 Microarray System for Contaminated Water Analysis
R830420C005 Material and Environmental Sustainability in Ceramic Processing
R830420C006 Interaction of Sealing Glasses with Metallic Interconnects in Solid Oxide and Polymer Fuel Cells
R830420C007 Preparation of Ceramic Glaze Waste for Recycling using Froth Flotation
R830420C008 Elimination of Lead from Ceramic Glazes by Refractive Index Tailoring
R830420C010 Nanostructured C6B: A Novel Boron Rich Carbon for H2 Storage
X832541C001 Microarray System for Contaminated Water Analysis
X832541C003 The Fining Behavior of Selectively Batched Commercial Glasses
X832541C004 The Use of Fly Ash in the Production of SiAlON based Structural Ceramics
X832541C005 Separation and Purification of Hydrogen From Mixed Gas Streams Using Hollow Glass Microspheres
X832541C006 Magnesium Rich Coatings for Corrosion Control of Reactive Metal Alloys
X832541C008 Tunneled Titanate Photocatalysts for Environmental Remediation and Hydrogen Generation
X832541C009 Material and Environmental Sustainability in Ceramic Processing
X832541C010 Robust, Spectrally Selective Ceramic Coatings for Recycled Solar Power Tubes
X832541C011 Recycling of Silicon-Wafers Production Wastes to SiAlON Based Ceramics with Improved Mechanical Properties