Technology Description: Agricultural Research Service (ARS) scientists in Illinois developed a way to stabilize bacterial strains used in ethanol production for improved efficiency. The technology uses two genes that are responsible for producing alcohol: dehydrogenase and pyruvate—which are effective for producing high ethanol concentration. Current organisms designed to increase ethanol production require adding antibiotics to maintain genetic stability and high ethanol production. However, this method has drawbacks because of environmental and economical concerns. ARS’s invention allows bacteria engineered with the two genes to grow, while maintaining high ethanol production without using antibiotics.
The two enzymes can be obtained from a variety of sources and transformed into different mutant hosts and expressed using a variety of promoters. The recombinant bacteria are effective for fermenting a variety of sugars from biomass or agricultural wastes to ethanol using conventional methods. Mutant strains possessing the two genes can be transformed using conventional genetic recombination methods.
Manufacturing companies that produce ethanol and alternative fuels, along with companies involved in manufacturing microorganisms could use this technology.
This technology has been well tested in laboratory studies. ARS is seeking a cooperative partner or licensee to scale-up the technology for commercial use.
Reference: A copy of this patent, USPN 6,280,986 (Docket #0174.96), issued
August 28, 2001, can be obtained from the U.S. Patent and Trademark Office Website (www.uspto.gov). Foreign rights are not available.
Inventors:
Bruce S. Dien
Fermentation Biotechnology Research Peoria, IL 61604-3999
Phone: (309) 681-6270
Fax: (309) 681-6427 dienb@ncaur.usda.gov |
Robert B. Hespell (Same as first inventor)
Rodney J. Bothast (Same as first inventor)
Herbert A. Wyckoff (Same as first inventor) |
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