Technology Description:
ARS researchers have developed a quick and easy extraction method to improve detection of antibodies, antigens, bacteria, and viruses. The purpose of this technology is to obtain clear aqueous samples in a few steps that can be applied to a variety of diagnostic tests. For example, this technique affords early, low cost detection of poultry diseases. Antigens and antibodies are not diluted; therefore, this allows for more sensitive testing. The advantages of this technology over existing technologies are quick extraction time (less than 30 minutes); clear, clean, concentrated or undiluted extract; high yield of extracted antigen or antibody; ability to use extract in conventional tests and fluorescence polarization tests; no enrichment step, and inactivation of organisms such as Salmonella. Existing technologies use harsh chemicals for extraction, purification methods that require numerous steps, and require more than three days for detection of Salmonella from eggs when serotyping is used.
Egg producer, poultry breeder, diagnostic, and vaccine companies could use this technology. The animal health industry will also benefit from this information.
Reference:
Please refer to patent application S.N. 11/445,091 (Docket #0110.06), “Extraction and Concentration Method,” which was filed on May 31, 2006, and is a continuation of S.N. 10/225,419 (Docket #0221.01), which was filed on August 22, 2002. Foreign rights are not available.
Inventors:
Henry D. Stone (Collaborator)
Egg Safety and Quality Research
950 College Station Road
Athens, GA 30605-2796
Phone: (706) 546-3034
Fax: (706) 546-3035
hstone@seprl.usda.gov
Peter S. Holt
Egg Safety and Quality Research
950 College Station Road
Athens, GA 30605
Phone: (706) 546-3442
Fax: (706) 546-3035
pholt@seprl.usda.gov
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Cam R. Greene
Exotic and Emerging Avian Viral Diseases
934 College Station Road
Athens, GA 30605
Phone: (706) 546-3441
Fax: (706) 546-3161
cgreene@seprl.usda.gov
Richard K. Gast
(Same as first inventor)
Phone: (706) 546-3445
Fax: (706) 546-3035
rgast@seprl.usda.gov
Kuhno Seo
OPDFB, CFSAN, FDA
500 Paint Branch Parkway
Room 4E-015, HFS-302
College Park, MD 20740 |
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