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E.O. Lawrence Berkeley National Laboratory
ABSTRACT:
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Berkeley Lab researchers Manouchehr Saljoughian, Hiromi Morimoto,
Chit Than, and Philip Williams have synthesized a series of patented
tritioacetylating reagents. These reagents may be used in a new
and facile method for the tritioacetylation, to very high specific
activities, of peptides and other biologically important molecules,
such as Co-enzyme A. Acetyl Co-enyzme A is an important metabolic
intermediate, and due to its high potential for the transfer of
an S-acetyl group, serves as a substrate for a wide variety of enzyme
catalyzed reactions. Acetyl Co-enzyme A plays a central role in
fatty acid and steroid biosyntheses, as well as in the tricarboxylic
acid cycle. The best characterized of Berkeley Labs new tritioacetylating
compounds, N-tritio-acetoxyphthalimide has many advantages over
standard reagents such as tritiated acetic acid and acetic anhydride:
both these substances suffer from low tritium content, volatility,
and poor chemical selectivity and reactivity. Berkeley Labs
N-tritioacetoxyphthalimide readily tritiates amino and sulfhydryl
groups in proteins; is quite easy to prepare; is stable; and is
active in DMSO, methanol, dioxane, and water.
STATUS:
- U.S. Patent #6,160,128. Available for licensing.
FOR MORE INFORMATION PLEASE SEE:
REFERENCE NUMBER: IB-1250
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CONTACT:
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Technology
Transfer Department
E.O. Lawrence Berkeley National Laboratory
MS 90-1070
Berkeley, CA 94720
(510) 486-6467 FAX: (510) 486-6457
TTD@lbl.gov |
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