U.S. Food & Drug Administration
Center for
Food
Safety &
Applied
Nutrition
Bacteriological
Analytical
Manual Online
January 2001
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R42
McFarland Nephelometer
(Return to Table of Contents)
Make suspensions of barium sulfate as follows:
- Prepare 1.0% solution of CP (chemically pure) sulfuric acid.
- Prepare 1.0% solution of CP barium chloride.
- Prepare 10 standards as follows:
Add (ml 1% BaCl2 solution) |
to (ml 1% H2SO4 solution) |
1 |
99 |
2 |
98 |
3 |
97 |
4 |
96 |
5 |
95 |
6 |
94 |
7 |
93 |
8 |
92 |
9 |
91 |
10 |
90 |
- Seal about 3 ml of each standard suspension of barium sulfate precipitate in small test tube. Select test tubes carefully for uniformity of
wall thickness, diameter, and color. (These preparations are available commercially from several laboratory supply firms.)
Hypertext Source: Bacteriological Analytical
Manual, 8th Edition, Revision A, 1998.
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