Gregg A. Howe
Research Interests
Molecular and Biochemical Basis of Plant-Insect Interactions
Research in our laboratory is aimed at understanding how plants respond to insect herbivory and other forms of wound stress. We use both tomato (Solanum lycopersicum) and Arabidopsis (Arabidopsis thaliana) as experimental model systems for three related areas of investigation: (1) we are elucidating the mechanism of synthesis and action of the plant hormone jasmonate; (2) we are studying how jasmonate-regulated defensive compounds thwart insect attack; and (3) we are studying the development and metabolic function of glandular trichomes in tomato. These projects provide training in several areas of modern plant biology, including: analysis of protein-protein and receptor-hormone interactions; transcriptional networks; plant development; genetics of plant-insect interactions; protein biochemistry/proteomics; metabolism and metabolomics; and crop improvement for insect resistance.
Molecular Mechanism of Jasmonate Signaling
Herbivorous insects use diverse feeding strategies to obtain nutrients from their host plants. Rather than acting as passive victims in these interactions, plants cope with herbivory through the production of myriad specialized metabolites and proteins that exert toxic or anti-feedant affects on herbivores, or volatile substances that act indirectly by attracting predators of the herbivore. This highly dynamic form of immunity is initiated by the recognition of insect oral secretions and signals from injured plant cells. The plant hormone jasmonate (JA) plays a conserved and central role in this process by regulating genome-wide changes in gene expression.
A long-term objective of our research is to elucidate the molecular mechanism by which JA controls gene expression. A combination of genetic, cell biological, molecular, and biochemical analyses indicates that the core signal transduction chain linking JA synthesis to hormone-induced changes in gene expression consists of four components: a bioactive JA signal, the SCF-type E3 ubiquitin ligase SCFCOI1, JAsmonate ZIM-domain (JAZ) repressor proteins that are targeted by SCFCOI1 for degradation via the ubiquitin/26S proteasome pathway, and transcription factors (TFs) that promote the expression of JA-responsive genes (Fig. 1). Recent studies from our lab indicate that the F-box protein COI1 is a critical component of the JA receptor, and that jasmonoyl-isoleucine (JA-Ile), an amino acid-conjugated form of JA, is a natural ligand for this receptor system. A major unanswered question we seek to address is how the specificity of receptor-ligand and JAZ-TF interactions regulates the diversity of JA-mediated responses.
Figure 1. JA regulates numerous physiological processes in response to environmental and developmental cues. FACs, fatty acid-amino acid conjugates; GLVs, green leafy volatiles. Figure modified from Howe and Jander (2008) Annu Rev Plant Biol 59: 41-66.
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Recent Publications
Koo AJ, Cooke TF, Howe GA. 2011. Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine. Proc Natl Acad Sci U S A. 108:9298-9303. Link to article
Gonzales-Vigil E, Bianchetti CM, Phillips GN Jr, Howe GA. 2011. Adaptive evolution of threonine deaminase in plant defense against insect herbivores. Proc Natl Acad Sci U S A.Apr 5;108(14):5897-902. Link to article
Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR, Kobayashi Y, Hsu FF, Sharon M, Browse J, He SY, Rizo J, Howe GA, Zheng N. 2010. Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature.468:400-405. Link to article
Howe GA. 2010. Ubiquitin ligase-coupled receptors extend their reach to jasmonate. Plant Physiol. 154(2):471-4. Link to article
Kang JH, Liu G, Shi F, Jones AD, Beaudry RM, Howe GA. 2010. The tomato odorless-2 mutant is defective in trichome-based production of diverse specialized metabolites and broad-spectrum resistance to insect herbivores. Plant Physiol. 154(1):262-72. Link to article
HS Chung, TF Cooke, CL Depew, LC Patel, N Ogawa, Y Kobayashi, GA Howe. 2010. Alternative splicing expands the repertoire of dominant JAZ repressors of jasmonate signaling. Plant Journal. 63 613-622. Link to publication
Koo AJ, Gao X, Jones AD, Howe GA. 2009. A rapid wound signal activates the systemic synthesis of bioactive jasmonates in Arabidopsis. Plant J. Sep;59(6):974-86. Link to publication
Koo AJ, Howe GA. 2009. The wound hormone jasmonate. Phytochemistry. Sep;70(13-14):1571-80. Link to publication
Chung HS, Niu Y, Browse J, Howe GA. 2009. Top hits in contemporary JAZ: an update on jasmonate signaling. Phytochemistry. Sep;70(13-14):1547-59. Link to publication
Chung HS, Howe GA. 2009. A Critical Role for the TIFY Motif in Repression of Jasmonate Signaling by a Stabilized Splice Variant of the JASMONATE ZIM-Domain Protein JAZ10 in Arabidopsis. Plant Cell. Jan;21(1):131-45. Link to publication
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