Genome Assembly

NCBI Contig Assembly and Annotation Process: The process used to assemble the contigs and annotate the sequence is complex and continuously being refined. The linked page gives a detailed description of the NCBI approach.

Data Preparation

• Input data includes both finished and draft genomic sequence data from GenBank
• Data is screened for contamination by bacterial and viral sequences
• Sequence is compared to other genomes, such as the mouse
• Repeats are masked

Contig Construction

• Clone layout stage
• Sequence building stage
• Melds of overlapping sequences formed and ordered
• Contigs are placed on a chromosome using sequence overlaps with mapped STS markers and paired BAC-end sequences.