Quinn TC, Kline R, Livingston R, Carella A, Moss M, Hutton N; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 78 (abstract no. WS-B35-4).
NIAID, Bethesda, MD.
OBJECTIVE: To reliably diagnose perinatally acquired HIV infection using an algorithm that incorporates two serologic assays for use in developing and developed countries. METHODS: 604 sera were sequentially obtained from 278 infants born to HIV-infected mothers and tested for HIV IgA antibodies and p24 Ag following acid dissociation. Infants were classified according to age and infection status after 15 mos. of age. Sensitivity and specificity of each test was determined separately and combined. RESULTS: 250 sera were from 78 HIV-infected children and 250 were from 130 uninfected children. 104 samples were from children of unknown HIV status. TABULAR DATA, SEE ABSTRACT VOLUME. The sensitivity of the IgA assay ranged from 20% at 3 mos. to 98.5% at > 12 mos. The p24 assay had a sensitivity of 100% at 3 mos. to 79.6% at > 12 mos. With assays combined, sensitivity and specificity at all time intervals was 100%. CONCLUSION: From these data an algorithm was developed that could reliably detect HIV infection in children based on screening all sera for p24 Ag followed by IgA testing of only the p24 Ag negative patients. Using these tests in tandem should provide both sensitivity and specificity independent of the age or antiviral use which might affect p24 Ag levels.
Publication Types:
Keywords:
- AIDS Serodiagnosis
- Acquired Immunodeficiency Syndrome
- Antigens
- Biological Assay
- Child
- Communicable Diseases
- Early Diagnosis
- Enzyme-Linked Immunosorbent Assay
- HIV Antibodies
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Immunologic Techniques
- Infant
- Laboratory Techniques and Procedures
- Sensitivity and Specificity
- immunology
- organization & administration
Other ID:
UI: 102205729
From Meeting Abstracts