Commercially Available STR Multiplex Kits
Kits from commercial sources are now
predominantly used by labs around the world for their ease of use
and high discriminatory power.
For further
information, see Butler, J.M. (2006) Genetics and genomics of core STR loci used
in human identity testing.
J. Forensic Sci. 51(2): 253-265.
[Explanation
of STR Multiplexes] [STR Fact Sheets]
STR Kits from Applied Biosystems
(Foster City, CA)
- AmpFlSTR®
Profiler Plus™ :
D3S1358, VWA, FGA,
Amelogenin,
D8S1179, D21S11, D18S51, D5S818,
D13S317, D7S820
- AmpFlSTR®
COfiler™ :
D3S1358,
D16S539, Amelogenin,
TH01, TPOX, CSF1PO, D7S820
- AmpFlSTR®
SGM Plus™:
D3S1358, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, D21S11, D18S51,
D19S433, TH01, FGA
- AmpFlSTR®
Identifiler™:
D8S1179,
D21S11, D7S820, CSF1PO, D3S1358,
TH01,
D13S317,
D16S539, D2S1338,
D19S433, VWA,
TPOX, D18S51,
Amelogenin, D5S818, FGA
- AmpFlSTR®
MiniFiler™:
D13S317, D7S820,
Amelogenin, D2S1338,
D21S11,
D16S539, D18S51, CSF1PO, FGA
- AmpFlSTR®
Yfiler™:
DYS456,
DYS389I,
DYS390,
DYS389II, DYS458, DYS19,
DYS385a/b,
DYS393, DYS391, DYS439,
DYS635,
DYS392, Y_GATA_H4,
DYS437, DYS438, DYS448
- AmpFlSTR®
Profiler™:
D3S1358, VWA, FGA,
Amelogenin,
TH01, TPOX, CSF1PO, D5S818,
D13S317, D7S820
- AmpFlSTR®
SEfiler™:
D3S1358, VWA,
D16S539, D2S1338,
Amelogenin,
D8S1179, SE33, D19S433, TH01, FGA,
D21S11, D18S51
- AmpFlSTR® Green I :
Amelogenin, TH01, TPOX, CSF1PO
- AmpFlSTR® Blue:
D3S1358, VWA, FGA
STR Kits from
Promega Corporation
(Madison, WI)
- PowerPlex® 16 :
D3S1358,
TH01, D21S11, D18S51,
Penta E, D5S818,
D13S317, D7S820,
D16S539, CSF1PO,
Penta D,
Amelogenin, VWA,
D8S1179, TPOX, FGA
- PowerPlex® Y:
DYS391,
DYS389I, DYS439,
DYS389II, DYS438,
DYS437, DYS19, DYS392,
DYS393, DYS390,
DYS385a/b
- PowerPlex® 1.2: almost same as
PowerPlex 1.1 but for ABI 310 instead of FMBIO
- PowerPlex® 1.1: for FMBIO users
- PowerPlex® 2.1: for FMBIO users
- PowerPlex® 16 BIO: same as PowerPlex 16
but for FMBIO users
- PowerPlex® ES:
D3S1358,
TH01, D21S11, D18S51, SE33,
Amelogenin, VWA,
D8S1179, FGA
- Silver Stain Systems:
- CTT: CSF1PO, TPOX, TH01
- CTTV : CSF1PO, TPOX, TH01, VWA
- FFV: F13A1, FESFPS, VWA
- FFFL : F13A1, FESFPS, F13B, LPL
- GammaSTRTM: D16S539, D7S820, D13S317, D5S818
STR Kits from
Biotype (Dresden, Germany)
-
Mentype® Quality Sensor
-
Mentype® Nonaplex I
-
Mentype® Nonaplex II
-
Mentype® Nonaplex Twin
-
Mentype® NonaplexQS
-
Mentype® NonaplexQS Twin
-
Mentype® Triplex DAA
-
Mentype® Triplex AFSQS
-
Mentype® Triplex TVD
-
Mentype® Argus Y-MHQS
-
Mentype® Argus X-UL: DXS8378,
DXS7132, HPRTB, and DXS7423
as well as Amelogenin
-
Mentype® Argus X-8:
- Humantype Chimera®: Amelogenin, D7S1517,
D3S1744, D12S391, D2S1360, D6S474, D8S1132, D5S2500, D18S51, D21S2055,
D10S2325, SE33, D4S2366
Explanation
of STR Multiplexes:
The desire to gain more information from a sample, coupled
with the need to limit consumption of a DNA sample where its
availability may be limited (such as evidence from a crime scene)
has led to the co-amplification and typing of multiple STR
systems. Multiplex PCR, which involves adding more
than one set of PCR primers to the reaction in order to target
multiple locations throughout the genome, is an ideal technique
for DNA typing because the probability of identical alleles in
two individuals decreases with an increase in the number of
polymorphic loci examined. The advent of fluorescent labeling
of the PCR primers permits the multiplexing of
STR loci which may have alleles that fall in the same size range by labeling the
overlapping loci with different colored fluorescent dyes that can then be
resolved spectrally.
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Last Updated:
06/28/2007