Uccini S, Sirianni MC, Vincenzi L, Stoppacciaro A, Capuano M, Masini C, Lesnoni La Parola I, Cerimele D, Ruco LP, Baroni CD; International Conference on AIDS.
Int Conf AIDS. 1996 Jul 7-12; 11: 97 (abstract no. We.B.3237).
Department of Experimental Medicine and Pathology, Rome, Italy. Fax: 011 39 6 494 0896.
Objective: To investigate the possibility that PB of KS patients contains circulating KS precursor cells. Methods: PBMC from 5 AIDS-KS and 11 classic KS patients, from 30 healthy volunteers, 5 HIV-1+ patients without KS, and from 15 patients affected by chronic dermatitis were cultured in 20% conditioned medium from PHA-stimulated PBL for 7-21 days. In positive cultures, adherent spindle cells were detached by trypsinization and were cytocentrifuged. Cytosmears were single and double immunostained with a panel of antibodies. The antigenic profile of PB-derived KS-like spindle cells was compared with that of KS-spindle cells present in 19 tissue biopsies of KS lesions. Results: Adherent spindle cells were obtained in PBMC cultures of 13/16 KS patients and in none of the controls. KS-like adherent cells co-expressed the endothelial antigens VE-cadherin/CD31 and the macrophage associated antigens Mannose Receptor/CD68/CD14. Their immunophenotype was similar to that detected in the spindle cells of the KS skin lesions. Moreover, we have demonstrated by double staining that PB-derived KS-like cells were CD45+/VE-cadherin+. Further evidence suggesting that KS spindle cells are related to the macrophage lineage was provided by the observation that greater than 95% of KS cells present in 19/19 KS tissue biopsies were intensely stained for Mannose Receptor which is a 175 kDa C-type lectin receptor selectively expressed by macrophage/dendritic cells. Conclusions: Our findings are consistent with the possibility that KS lesions derive from local accumulation and proliferation of bone marrow-derived circulating precursor cells, probably belonging to a particular subset of macrophages.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigens, CD
- Antigens, CD14
- Antigens, CD31
- Antigens, CD45
- Antigens, Differentiation, Myelomonocytic
- CD68 antigen, human
- Hematopoietic Stem Cells
- Humans
- Immunophenotyping
- Lectins
- Lectins, C-Type
- Macrophages
- Mannose-Binding Lectins
- Nitric Oxide Synthase
- Receptors, Cell Surface
- cerebellar soluble lectin
- immunology
- mannose receptor
Other ID:
UI: 102219488
From Meeting Abstracts