Inclusion Criteria:

-Chronic severe CAD patients fulfilling all the following criteria are considered suitable for inclusion in the study.

1. At least 6 months since last episode of myocardial infarction or at least 3 months since initial anginal episode.

2. Patients fulfilling either of the following criteria based on the extent of CAD by coronary angiography and LVEF by echocardiography.

   • Patients with single vessel CAD and LVEF < 50%
   • Patients with multivessel CAD
3. Reversible myocardial ischemia as revealed by sestamibi SPECT stress myocardial scintigraphy.
4. Patients for whom angioplasty and bypass are not indicated because of anatomical or procedural reasons or frequent reocclusion/restenosis following traditional revascularization.
5. Age is between 20 and 80 (at time of consent).
6. Exercise tolerance time (ETT) duration ≥ 3 minutes and < 13 minutes on a modified Bruce protocol on 2 consecutive tests (> 24 hours but < 2 weeks apart), with the difference between the 2 exercise times within 25% of their mean (Patients should not be informed of exercise restrictions required for entry into the study).
7. Patients who can give informed consent themselves in writing.

Exclusion Criteria:

• Any one of the following exclusion criteria is sufficient to disqualify a patient from the study.

  1. Sustained ventricular tachycardia in a 24-hour ECG.
  2. Chronic atrial fibrillation.
  3. Less than 6 months since last episode of cerebral infarction.
  4. Less than 6 months since last coronary angioplasty or less than 3 months since last bypass surgery.
  5. Patients with unstable angina, with a treatment rating of 3 in the Braunwald system (refer to 5.4.), but a severity of III and a clinical rating of B or C.
  6. Presence of left ventricular thrombus by echocardiography
  7. Patients with a malignant tumor*.
  8. Patients with diabetic proliferating retinopathy** (new Fukuda classification BI to BV).
  9. Patients with chronic rheumatoid arthritis.
  10. Patients with a history of severe allergic reactions or side effects to G-CSF preparations or apheresis.
  11. Patients with hematological disease (leukemia, myeloproliferative disease, or myelodysplastic syndromes).
  12. Patients currently suffering from or having a history of interstitial pneumonitis.
  13. Patients for whom cranial MRA reveals cerebral aneurysm.
  14. Patients for whom abdominal CT or ultrasonography reveals splenomegaly.
  15. Patients with cirrhosis of the liver.
  16. Patients who cannot discontinue Warfarin.
  17. Leukocytes less than 4,000/µL or exceeding 10,000/µL.
  18. Platelets less than 100,000/µL.
  19. Hemoglobin less than 10 g/dL.
  20. AST (GOT) exceeding 100 IU/L or ALT (GPT) exceeding 100 IU/L.
  21. Patients for whom it is impossible to perform both cardiac MRI and left ventriculography (LVG) (see 9.2.4 for cardiac MRI details and 9.2.9 for LVG details).
  22. Patients with gate disturbance for reasons other than CAD (such as critical limb ischemia, sciatic neuralgia, or vasculitis), making exercise tolerance evaluation on a treadmill with stress ECG difficult.
  23. Pregnant or nursing patients, those who may be pregnant, or those who plan on becoming pregnant before the end of the study period.
  24. Any other reason that the Clinical Supervisors or Clinical Researchers may have for considering a case unsuitable for the study.