Sperm Detection and Separation
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Abstracts
- DNA (NCJRS)
- Forensics (NCJRS)
- Pub Med
- DNA Scientific Journals (NCSTL)
- Links to Forensic Journals (NCSTL)
Science.gov (search on "forensic dna)
Forensic Science Communications
Federal Bureau of Investigation
Description
The detection of sperm and separation of sperm cells from the victim's DNA is crucial to identifying and characterizing the perpetrator's DNA profile. Improved automation of sperm detection and separation will enable more rape kits to be processed in a timely manner to help solve sexual assault crimes more effectively. Traditionally sperm cells are identified visually in evidentiary samples through light microscopy. New techniques are under development to aid improved visualization of sperm heads and tails with fluorescent staining as well as sperm cell isolation through laser capture microdissection.
Research Projects
The table below presents awards funded both prior to and under the the President's DNA Initiative, which began in 2004.
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| Year | Title | Awardee | Amount | Award Number |
|---|---|---|---|---|
| 2008 | Holographic Optical Trapping in Forensic Research and Development: Application to Rape Kit Analysis | Arryx, Inc | $252,026 | 2008-DN-BX-K123 |
| 2008 | Detection and quantitation of spermatozoa | Paternity Testing Corporation | $361,651 | 2008-IJ-CX-K007 |
| 2008 | Microdevice for Sperm and Sperm DNA Isolation | Oak Ridge National Laboratory | $445,673 | 2008-IJ-R-134 |
| 2007 | Sperm Capture Using Aptamer-Based Technology | Denver, City and County of | $370,813 | 2007-DN-BX-K143 |
| 2006 | Laser Microdissection as a Technique to Isolate Sperm Cells and Improve the Analysis of Touch Evidence | Bode Technology Group, Inc. | $191,429 | 2006-DN-BX-K032 |
| 2005 | Development of a Procedure for Dielectrophoretic Separation of Sperm and Epithelial Cells for Application to Sexual Assault Case Evidence | California Department of Justice | $205,407 | 2005-DA-BX-K001 |
| 2004 | Development of an Automated System to Detect Spermatozoa on Laboratory Slides | Vermont Department of Public Safety | $214,629 | 2004-DN-BX-K003 |
| 2003 | Development of High-Throughput Method to Isolate Sperm DNA | Connecticut Department of Public Safety | $123,300 | 2003-IJ-CX-K103 |
Research Articles and Reports
Select a title to view an abstract from PubMed database (National Library of Medicine's) unless a different file type is indicated.
Sort the table by selecting a column head. Sort multiple columns by holding down "Shift" as you select heads.
| Title | Authors | Publication | Year | Awardee | Award Number |
|---|---|---|---|---|---|
| Laser Microdissection Separation of Pure Spermatozoa Populations from Mixed Cell Samples for Forensic DNA Analysis (pdf, 100 pages) | Christine T. Sanders; Emily K. Reisenbigler; Daniel A. Peterson | Final Grant Report | 2007 | Rosalind Franklin University of Medicine and Science | 2004-DN-BX-K215 |
| Development of a High-Throughput Method to Isolate Sperm DNA in Sexual Assault Cases (pdf, 31 pages) | Carll Ladd; Eric Carita; Elaine Pagliaro; Alex Garvin; Andrew Crumbie; Henry Lee | Final Grant Report | 2006 | Connecticut Department of Public Safety | 2003-IJ-CX-K103 |
| Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis | Sanders CT, Sanches N, Ballantyne J, Peterson DA | J Forensic Sci. 2006 Jul; 51(4):748-57 | 2006 | Rosalind Franklin University of Medicine and Science | 2004-DN-BX-K215 |
