• INCLUSION CRITERIA:

The following eligibility criteria apply to initial enrollment into the study:

A participant must meet all of the following criteria:

1. 18 to 60 years old
2. Available for clinical follow-up through Week 32
3. Able to provide proof of identity to the satisfaction of the study clinician completing the enrollment process
4. Complete an AoU prior to enrollment and verbalize understanding of all questions answered incorrectly
5. Able and willing to complete the informed consent process
6. Willing to donate blood for sample storage to be used for future research
7. In good general health without clinically significant medical history

8. Physical examination and laboratory results without clinically significant findings and a body mass index (BMI) less than 40 within the 28 days prior to enrollment

   LABORATORY CRITERIA WITHIN 28 DAYS PRIOR TO ENROLLMENT:

9. Hemoglobin greater than or equal to 11.5 g/dL for women; greater than or equal to 13.5 g/dL for men
10. White blood cells (WBC) = 3,300-12,000 cells/mm3
11. Differential either within institutional normal range or accompanied by site physician approval
12. Total lymphocyte count greater than or equal to 800 cells/mm3
13. Platelets = 125,000 - 400,000/mm3
14. Alanine aminotransferase (ALT) less than 1.25 upper limit of normal
15. Serum creatinine less than or equal to 1 x upper limits of normal (less than or equal to1.3 mg/dL for females; less than or equal to 1.4 mg/dL for males)
16. Normal urinalysis defined as negative glucose, negative or trace protein and no clinically significant blood in the urine
17. Negative FDA-approved HIV blood test. [Note: Results of HIV ELISA will be documented, but a negative HIV polymerase chain reaction (PCR) test result will be sufficient for eligibility screening of subjects with positive HIV ELISA that is due to prior participation in an HIV vaccine study]
18. Negative hepatitis B surface antigen (HBsAg)
19. Negative anti-HCV and negative hepatitis C virus (HCV) PCR
20. Partial thromboplastin time (PTT) within institutional normal range

21. Prothrombin time (PT) less than or equal to upper limit of normal

    FEMALE-SPECIFIC CRITERIA:

22. Negative Beta-HCG pregnancy test (urine or serum) for women presumed to be of reproductive potential

23. A female participant must meet one of the following criteria:

    • No reproductive potential because of menopause [one year without menses] or because of a hysterectomy, bilateral oophorectomy, or tubal ligation,

OR

• Participant agrees to be heterosexually inactive at least 21 days prior to enrollment and through Week 32 of the study,

OR

• Participant agrees to consistently practice contraception at least 21 days prior to enrollment and through

Week 32 of the study by one of the following methods:

1. condoms, male or female, with or without a spermicide
2. diaphragm or cervical cap with spermicide
3. intrauterine device
4. contraceptive pills or patch, Norplant, Depo-Provera or any other FDA-approved contraceptive method

5. male partner has previously undergone a vasectomy

   EXCLUSION CRITERIA:

   A SUBJECT WILL BE EXCLUDED IF ONE OR MORE OF THE FOLLOWING CONDITIONS APPLY:

   Women:

   1. Breast-feeding or planning to become pregnant during the first 32 weeks after enrollment

      SUBJECT HAS RECEIVED ANY OF THE FOLLOWING SUBSTANCES:

   2. Investigational Ebola vaccine in a prior clinical trial
   3. Immunosuppressive medications, cytotoxic medications, inhaled corticosteroids, or long-acting beta-agonists within the 12 weeks prior to enrollment. [With the exceptions that use of corticosteroid nasal spray for rhinitis, topical corticosteroids for an acute uncomplicated dermatitis, or short-acting beta-agonists in controlled asthmatics; or a short course (duration of 10 days or less, or a single injection) of corticosteroids for a non-chronic condition at least 2 weeks prior to enrollment in this study will not exclude study participation.]
   4. Blood products within 120 days prior to HIV screening
   5. Immunoglobulin within 60 days prior to HIV screening
   6. Live attenuated vaccines within 30 days prior to initial study vaccine administration
   7. Investigational research agents within 30 days prior to initial study vaccine administration
   8. Medically indicated subunit or killed vaccines, e.g. influenza, pneumococcal, or allergy treatment with antigen injections, within 14 days of study vaccine administration

   9. Current anti-tuberculosis prophylaxis or therapy

      SUBJECT HAS A HISTORY OF ANY OF THE FOLLOWING CLINICALLY SIGNIFICANT CONDITIONS:

   10. Serious adverse reactions to vaccines such as anaphylaxis, urticaria (hives), respiratory difficulty, angioedema, or abdominal pain
   11. Idiopathic urticaria within the past 2 years
   12. Autoimmune disease or immunodeficiency
   13. Asthma that is unstable or required emergent care, urgent care, hospitalization or intubation during the past 2 years or that requires the use of oral or parenteral corticosteroids
   14. Diabetes mellitus (type I or II), with the exception of gestational diabetes
   15. History of thyroidectomy or thyroid disease that required medication within the past 12 months
   16. A history of hereditary angioedema (HAE), acquired angioedema (AAE), or idiopathic forms of angioedema
   17. Hypertension that is not well controlled by medication or blood pressure that is more than 145/95 at enrollment
   18. Bleeding disorder diagnosed by a doctor (e.g. factor deficiency, coagulopathy, or platelet disorder requiring special precautions), significant bruising or bleeding difficulties with IM injections or blood draws, or routine use of anticoagulant medications
   19. Malignancy that is active or treated malignancy for which there is not reasonable assurance of sustained cure or malignancy that is likely to recur during the period of the study
   20. Seizure disorder other than: 1) febrile seizures under the age of 2 years, 2) seizures secondary to alcohol withdrawal more than 3 years ago, or 3) a singular seizure not requiring treatment within the last 3 years
   21. Asplenia, functional asplenia or any condition resulting in the absence or removal of the spleen
   22. Allergic reaction to aminoglycoside antibiotics
   23. Psychiatric condition that precludes compliance with the protocol; past or present psychoses; past or present bipolar disorder; disorder requiring lithium; or within 5 years prior to enrollment, a history of suicide plan or attempt
   24. Any medical, psychiatric, social condition, occupational reason or other responsibility that, in the judgment of the investigator, is a contraindication to protocol participation or impairs a subject's ability to give informed consent

   Eligibility for the Optional 4th Study Injection:

   Eligibility to receive a 4th injection requires the original consent (Appendix I) to be reviewed again with a study clinician, as well as the consent for the optional 4th injection (Appendix V) to be reviewed and signed.

   To be eligible the study subject must meet the following criteria:

   • Has completed the 3-injection schedule for the Group in which he/she was enrolled.
   • Has remained in follow-up through Study Week 32 without any serious adverse events.
   • Meets the original study eligibility criteria for hemoglobin, platelets. PT. PTT. Creatinine and ALT within the 28 days prior to the 4th injection.
   • Assessed as continuing to have a healthy vaccine study volunteer status by recent interim history and appropriate physical assessments.
   • If a woman of reproductive potential, is willing to continue with a pregnancy prevention method (from among those meeting the original study eligibility) through 12 weeks after the injection and is confirmed as not pregnant on the day of the 4th injection prior to administration of the injection.
   • Available for clinical follow-up through 12 weeks after the 4th injection.